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1

CamCon - projekt ograniczenia występowania bakterii Campylobacter w stadach brojlerów jako element zwalczania kampylobakteriozy

100%
Kania I., Kusyk P., Wieczorek K., Osek J.
Polskie Drobiarstwo
|
2012
|
tom 19
|
nr 01
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics

88%
Stojecki K., Sroka J., Karamon J., Kusyk P., Cencek T.
Polish Journal of Veterinary Sciences
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2014
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tom 17
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nr 1
Giardia intestinalis is a widespread parasitic protozoa which has great significance as a public health threat. Molecular diagnostics of stool sample can be unreliable because of the presence of inhibitors of enzymatic reactions. The aim of this study was to determine the effectiveness of selected pre-treatment methods of fecal samples for further PCR-based diagnostics of G. intestinalis, and the effect of each component of pre-treatment solutions on PCR reactions. Seven stool concentration techniques were compared. The results showed that the most efficient concentration method for stool sample preparation for detection of G. intestinalis by PCR is centrifugal flotation with Percoll (with saturated NaNO₃ as the flotation solution). This method is relatively inexpensive, less labor-intensive, and suitable for epidemiological monitoring and clinical investigations.
3

Gastrointestinal helminths of raccoons (Procyon lotor) in western Poland (Lubuskie province) - with particular regard to Baylisascaris procyonis

88%
Karamon J., Kochanowski M., Cencek T., Bartoszewicz M., Kusyk P.
Bulletin of the Veterinary Institute in Puławy
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2014
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tom 58
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nr 4
The aim of the study was to estimate the prevalence of gastrointestinal parasites in raccoons with particular regard to zoonotic parasites. Fifty-five raccoons, hunted or found dead on roads, were examined. The small and large intestines were collected from all raccoons and, additionally, the stomach was collected from 43 animals. The samples were examined with the use of sedimentation and counting technique. The intestines and stomach were examined separately. Samples of raccoon faeces were collected from their environment localised in Słubice district, Lubuskie province (Poland). The samples were collected once a month in 2012. In total, 154 faecal samples were obtained and examined with the use of McMaster flotation technique. The following parasites were detected in the intestinal and stomach contents: tapeworms Mesocestoides sp. (67.3%), Echinostomatidae flukes (34.5%), and nematodes Capillaria sp. (25.5%). Moreover, Acanthocephala were found in the intestines of three raccoons. The highest intensity of infection were observed in case of Mesocestoides sp. Mesocestoides sp. and Echinostomatidae were found statistically more often in the intestines than in the stomach. In the case of these two parasites, there was positive correlation between the intensity of infection in the intestines and the presence of the same parasites in the stomach. Moreover, significantly higher prevalence and intensity of Mesocestoides sp. in males than in females were also observed. Faecal samples contained Baylisascaris procyonis eggs (mean 60 epg). These eggs were found in three samples collected in November and December. Furthermore, in some faecal samples eggs of flukes, Capillaria sp., Mesocystoides sp., and coccidian oocy sts were found. It is one of rare reports concerning Baylisascaris procyonis in Poland confirming the presence of this dangerous parasite in Polish raccoon population.
4
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Study on prevalence of Toxoplasma gondii infection in free-living animals in Poland

64%
Sroka J., Karamon J., Bilska-Zajac E., Stojecki K., Rozycki M., Kusyk P., Wojcik-Fatla A., Zajac V., Cencek T.
Annals of Parasitology
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2016
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tom 62
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nr Suppl.
5

Potential role of beavers (Castor fiber) in contamination of water in the Masurian Lake District (north-eastern Poland) with protozoan parasites Cryptosporidium spp. and Giardia duodenalis

51%
Sroka J., Gizejewski Z., Wojcik-Fatla A., Stojecki K., Bilska-Zajac E., Dutkiewicz J., Cencek T., Karamon J., Zajac V., Kusyk P., Dabrowska J., Kochanowski M.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 2
The purpose of this study was to assess the possible influence of beavers on the contamination of lake water with zoonotic parasites Giardia duodenalis and Cryptosporidium spp., with respect to the risk to human health. A total of 79 water samples were taken around the habitats of beavers from 14 localities situated in the recreational Masurian Lake District (north-eastern Poland). Water was sampled in the spring and autumn seasons, at different distances from beavers' lodges (0-2, 10, 30, and 50 m). The samples were examined for the presence of (oo)cysts of zoonotic protozoa Giardia duodenalis and Cryptosporidium spp. by direct fluorescence assay (DFA) and by nested and real time PCR. By DFA, the presence of Giardia cysts was found in 36 samples (45.6%) and the presence of Cryptosporidium oocysts in 26 samples (32.9%). Numbers of Giardia cysts, Cryptosporidium oocysts, and summarised (oo)cysts of both parasites showed a significant variation depending on locality. The numbers of Giardia cysts significantly decreased with the distance from beavers' lodges while the numbers of Cryptosporidium oocy sts did not show such dependence. The amount of Giardia cysts in samples collected in spring was approximately 3 tiir.es higher than in autumn. Conversely , a larger number of Cryptosporidium oocy sts were detected in samples collected in autum than in spring. By PCR, Giardia DNA was found in 38 samples (48.1%) whereas DNA of Cryptosporidium was found in on samples (8.9%). Eleven Giardia isolates were subjected to phylogenetic analy sis by restriction fragment length poly morphism PCR or sequencing which evidenced their belonging to zoonotic assemblages: A (3 isolates) and B (8 isolates). In conclusion, water in the vicinity of beavers' lodges in the tested region was markedly contaminated with (oo)cysts of Giardia duodenalis and Cryptosporidium spp., which confirms the potential role of beavers as a reservoir of these parasites and indicates a need for implementation of appropriate preventive measures to protect tourists' health.
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