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The objective of this study was to estimate a herd-level seroprevalence of bovine herpesvirus type 1 (BHV-1) in herds with clinical symptoms of the respiratory tract. Eighty-three herds with suspected BHV-1 infection were selected and divided into two categories with respect to their size: small (n=27) and large herds (n=56). Samples were collected from calves, heifers and cows older than 24 months. Seroprevalence was determined using the gB ELISA test. The herd level seroprevalence was estimated as 53% (44/83) in the tested herds, 11.1% (3/27) in the small herds and 73.2% (41/56) in the large herds. Our study suggests that the current biosecurity measures still warrant improvement.
The spectrum of Chlamydia detected in swine is very wide and includes species belonging to the family Chlamydiaceae. Systematic research indicates a different participation of individual species in disease syndromes. Disease cases are most frequently ascribed to infections with Ch. trachomatis, Ch. suis, Ch. psittaci, Ch. abortus, Ch. pecorum, Ch. pneumoniae. The problem of infections related to the occurrence of Chlamydia is widespread and associated with a variety of disease symptoms resulting in significant economic losses. At present the diagnostics is carried out by the National Reference Laboratory PIW-PIB in Pu³awy, and the percentage of positive seroreagents in the years 2008, 2009 and 2010 amounted to 0.4%, 0.47% and 1.65%, respectively.
Infection with bovine viral diarrhea virus is widespread in cattle populations all over the world and thus results in significant economic and breeding losses in herds of various sizes. The studies were conducted on 270 dairy cattle herds that have never been vaccinated against BVDV. Altogether 3732 samples were tested. Antibodies to BVDV were detected in 53.9% of herds. The correlation between herd size and seroprevalence was statistically significant and equal to 0.319 (p < 0.001). Analysis of clinical signs of BVD commonly observed in dairy cattle made it possible to conclude that a sudden drop of milk yield (OR = 2.037), diarrhea in calves (OR = 1.422), emaciation in young animals (OR=1.774) and emaciation in adults (OR = 1.715) were observed much more frequently in infected than uninfected herds. No increase was noted in the occurrence of other clinical signs regarded as typical for infected herds, such as spontaneous abortions and fetopathies in cows or respiratory and alimentary tract disorders.
The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis.
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