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Salt usually stresses plants in two ways, osmotic stress and ion toxicity. Plant responds to salinity in two distinct phases through time. It is known that silicon (Si) could alleviate salt stress by decreasing the Na⁺ accumulated in the leaf. In order to determine the function of Si in the two-phase growth response (osmotic and ion toxicity) to salinity, we selected the wheat cultivar ‘‘Changwu 134’’ out of 10 wheat cultivars, and confirmed that it responds to salinity in two distinct phases through time. The fresh weight, leaf area, and leaf Na⁺ concentration were measured during 31 days of 120 mM NaCl supplemented with 1 mM Si treatment. The results revealed that the growth of plants under salinity conditions both with and without Si application were in accordance with the two-phase growth model. Si alleviated the salt stress in the both two-phase growth, but the alleviative effects were more pronounced in the osmotic stress phase than ion toxicity phase. These results clearly showed that Si can enhance plant salt tolerance by alleviating the salt-induced osmotic stress.
Immobilized sulfate-reducing bacteria (SRB) in polyvinyl alcohol (PVA)-sodium alginate matrix were applied as biosorbents to remove cadmium (Cd) from aqueous solutions. Multiple characterization techniques including scanning electron microscope (SEM)-energy dispersive spectrometer (EDS), and Fourier transform infrared (FT-IR) spectra indicate that immobilized beads provided a suitable microenvironment for SRB. Performance tests show that Cd removal was highly affected by pH value and temperature, with optimum temperature at 35ºC and pH value of 8.0. A pseudo second-order model was applied to describe the adsorption kinetic. FT-IR and x-ray photoelectron spectroscopy (XPS) analyses imply that biosorption, sulfide, and hydroxide precipitation are the main mechanisms for removing Cd. The immobilized SRB beads have great potential for remediating Cd-containing wastewater.
Accumulation of reactive oxygen species (ROS) causes oxidative stress under adverse environmental conditions, such as salinity. Ethylene decreases accumulation of ROS induced by salinity, but the mechanism is still unclear. To examine the interactions between salinity and ROS accumulation and the possible role of ethylene metabolism in regulation, we used mutant ein2-5 in Arabidopsis with loss of function in EIN2. The mutant is compared to the wild-type Col-0, completely insensitivity to ethylene at the morphological, physiological and molecular levels. The oxidative responses of the wild type and mutant to salinity were compared. Loss-of-function of EIN2 enhanced sensitivity to salinity, implying that EIN2 is required for plant response to salinity. Furthermore, salinity resulted in accumulation of large amounts of ROS in ein2-5 seedlings when compared with Col-0, suggesting that the loss-of-function of EIN2 exaggerates oxidative stress induced by salinity. Activities of the antioxidant enzymes SOD, POD and CAT decreased significantly in ein2-5 under salinity when compared with Col-0 plants. The expression profiles of the genes Fe-SOD, PODs and CAT1, which code for ROS scavenging enzymes were severely decreased in ein2-5 under salinity compared with Col-0, suggesting that EIN2 was involved in regulating expression of these genes. Taken together, our results demonstrate that loss-of-function of EIN2 increased oxidative stress induced by salinity and that EIN2 is involved in modulating ROS accumulation, at least in part, by decreasing activities of ROS-scavenging enzymes.
Background. 6-Gingerol is one of the most pharmacologically active and abundant components in ginger, which has a wide array of biochemical and pharmacologic activities. In recent years, the application of microwave-assisted extraction (MAE) for obtaining bioactive compounds from plant materials has shown tremendous research interest and potential. In this study, an efficient microwave-assisted extraction (MAE) techniąue was developed to extract 6-gingerol from ginger. The extraction efficiency of MAE was also compared with conventional extraction techniques. Material and methods. Fresh gingers (Zingiber officinale Rosc.) were harvested at commercial maturity (originally from Shandong, laiwu, China). In single-factor experiments for the recovery of 6-gingerol, proper ranges of ratio of liquid to solid, ethanol proportion, microwave power, extraction time were determined. Based on the values obtained in single-factor experiments, a Box-Behnken design (BBD) was applied to determine the best combination of extraction variables on the yield of 6-gingerol. Results. The optimum extraction conditions were as follows: microwave power 528 W, ratio of liquid to solid 26 mL g1, extraction time 31 s and ethanol proportion 78%. Furthermore, more 6-gingerol and total polyphenols contents were extracted by MAE than conventional methods including Maceration (MAC), Stirring Extraction (SE), Heat reflux extraction (HRE), Ultrasound-assisted extraction (UAE), as well as the antioxidant capacity. Conclusion. Microwave-assisted extraction showed obvious advantages in terms of high extraction efficiency and antioxidant activity of extract within shortest extraction time. Scanning electron microscopy (SEM) images of ginger powder materials after different extractions were obtained to provide visual evidence of the disruption effect. To our best knowledge, this is the first report about usage of MAE of 6-gingerol extraction from ginger, which could be referenced for the extraction of other active compounds from herbal plants.
The Arabidopsis Ethylene-Insensitive3 (EIN3) has received attention recently and has been shown to be involved in the regulation of multitude of responses ranging from biotic stress defense and development to hormone interaction. To better understand the roles of EIN3 in plants response to salinity stress during germination and postgermination development, seeds of two EIN3 deficient mutant and a EIN3 overexpression mutant of Arabidopsis were analyzed under salinity and compared with Col-0 as control. The results showed that the ein3-1eil1-1 double mutant (lacking EIN3 and EIN3-Like1) and ein3-1 (lacking EIN3) were hypersensitive to high salinity ([150 mM NaCl), while EIN3 overexpression mutant (EIN3ox) displayed enhanced tolerance, indicating that EIN3 plays important roles during seed germination under salinity. In addition, we also found that the two EIN3 deficient mutant seedlings accumulate high levels of hydrogen peroxide (H2O2), which was thought to be an inhibitor of germination under salinity before, suggesting that EIN3 may function as a negative regulator of reactive oxygen species metabolism in germinating seeds under salinity. Taken together, our studies provide insights that EIN3 promotes seed germination under salinity, at least in part, through modulating concentration of H2O2 in germinating seeds.
The current study evaluated appetite-related gene expression of neuropeptide Y (NPY), orexin, type 2 orexin receptor (OrexinR2), proopiomelanocortin (POMC), melanocortin-4 receptor (MC4R), AMP-activated protein kinase α2 subunit (AMPKα2) and long-form leptin receptor (LeptinRb) in hypothalamus in response to copper (Cu) supplementation. One hundred crossbred pigs were assigned to four groups of 25 pigs, each comprising five replicates of 5 animals. Groups were then randomly assigned to treatments consisted of 1. control (10 mg/kg CuSO4), 2. 100 mg/kg CuSO4, 3. 175 mg/kg CuSO4, 4. 250 mg/kg CuSO4. On d 21 of the experiment 5 pigs from each group were slaughtered and the hypothalami were collected for determination of appetite-regulating genes mRNA expression level. The results showed that average daily feed intake and average daily gain were higher (P<0.05) in 250 and 175, 250 mg/kg Cu supplemented groups, respectively, than in the 10 mg/kg group. Feed:gain ratio was lower in pigs fed the diets with 250 mg/kg Cu (P<0.05) than in the 10 mg/kg group. Furthermore, the abundances of NPY mRNA in hypothalamus were higher in 250 mg/kg Cu supplemented groups (P<0.05), whereas the abundances of POMC and LeptinRb mRNA were significantly reduced (P<0.05) in 170, 250 mg/kg and 100, 175, 250 mg/kg Cu supplemented groups, respectively. No difference was found for AMPKα2, MC4R, orexin, OrexinR2 mRNA expression (P>0.05). These data suggest that down-regulation of LeptinRb mRNA expression might contribute to the stimulation of feeding of high dietary Cu supplementation via regulation of NPY and POMC mRNA expression.
Nansi Lake, a typical shallow and macrophyte-dominated lake in south Shandong Province, China, has a total surface area of 1,266 km2 and is the largest and most important freshwater reservoir in northern China for the eastern route of the South-North Water Diversion (SNWD) project, in which the water in Yangtze River will be transported more than 1,100 km from Yangzhou to Tianjin and Beijing. A water-level fluctuating zone (WLFZ) near Nansi Lake will be formed when the SNWD project begins to transport water flowing through the lake. Phosphorus fractions and adsorption-release characterization for soil samples from four typical lands (reed, wood, maize, and soybean) were conducted to investigate soil phosphorus stability. In addition, a soil submerged experiment was performed to simulate phosphorus release under submerged conditions. Phosphorus adsorption-desorption equilibrium concentrations (CEPC) of four lands were 0.006, 0.089, 0 .110, and 0.287 mg L-1, respectively, which means that maize, soybean, and woodland had higher potentials than reed land for phosphorus releasing to the overlaying water. Submerged experiment results showed that the quantities of p hosphorus released from reed, wood, maize, and soybean soils were -0.14, 0.06, 0.12, and 0.97 mg kg-1, respectively. Soils in the reed land adsorbed phosphorus from overlying water, but the other soils released phosphorus into the water. Thus, in order to decrease the phosphorus releasing quantity from the wood, soybean, and maize land under the conditions of submerged lands at water diversion time, the land uses of wood, soybean, and maize should be switched to land for planting reed.
As scattered elements, thallium (Tl) contamination poses a significant threat to human health due its high toxicity. Sulfate-reducing bacteria (SRB) embedded in PVA-sodium alginate matrix was utilized as a novel bio-remover to remove Tl from aqueous solution. The effect of pH, temperature, and initial Tl concentration on removal capacity of immobilized beads were studied and discussed in this work. The optimum bio-removal conditions were at pH value of 6, temperature of 35ºC, and initial Tl concentration of 50 mg.L⁻¹. The pseudo second-order model for Tl adsorption was applicable to all the removal data over the entire time range and intralayer diffusion was not the only rate-determining step. The bio-removal data conformed well to Langmuir isotherm model. Fourier transform infrared spectroscopy showed that sulfate reduction played an important role in Tl removal. The groups of carboxylate radical might be involved in sulfate reduction reaction.
Although the Qinghai-Tibetan Plateau (QTP) has experienced striking warming during the past century, information on how soil carbon (C) and nitrogen (N) pools of the alpine regions on the QTP respond to long-term warming is scarce. The aims of this study were to assess the response of soil organic C (SOC), total N (TN), labile C and N – including microbial biomass C (MBC) and N (MBN), inorganic N (Ninorg), dissolved organic C (DOC), and N (DON) – to 15-year experimental warming in an alpine region (Kobresia meadow and Potentilla scrubland), on the northeastern QTP using open-top chambers (OTCs). Fifteen-year experimental warming had no effect on SOC and TN concentrations and storage at 0-30 cm soil depth, either in Kobresia meadow or Potentilla scrubland habitat, which might be related to the low temperature increase and the unchanged water content. Long-term warming obviously affected soil labile C and N and their contributions to SOC and TN, especially in the meadow habitat, but the values were low, thus the variation of the labile C and N was not enough to influence total C and N storage. The C and N pools were shown to be controlled by different controlling factors, and scrubland was more stable than the meadow ecosystem confronting the change of environment.
Pre-operative treatment with recombinant human erythropoietin may improve aortic stenosis patients' condition, including anemia and/or cardiac dysfunction, for subjecting to aortic valve replacement. In this study, we tested this hypothesis in a mouse model of aortic stenosis. Adult male mice were subjected to either aortic stenosis created by aortic ligature or sham operation. Aortic stenosis for 4 weeks caused cardiac hypertrophy, pulmonary congestion and left ventricular dysfunction. It was associated with increased levels of tumor necrosis factor-a in serum and myocardium, and reduced levels of interleukin-10 in myocardium but not in serum. Mytocyte apoptosis rate, level of cleaved caspase 3, activity of nuclear factor-B and expression of p38-MAPK pathway were also elevated. Erythropoietin treatment increased hematocrit but did not prevent the development of cardiac hypertrophy. It, however, reduced the apoptosis, prevented the increases in tumor necrosis factor-, nuclear factor-B activation and phosphorylation of p38, and attenuated the increases in lung weight, the decreases in LVEF and LVFS, and the increases in LVDd and LVDs. In conclusion recombinant human erythropoietin has cardioprotective effects in maladaptive cardiac hypertrophy by inhibiting nuclear factor-B activation, phosphorylation of p38-MAPK pathway, and production of tumor necrosis factor-, together leading to a reduced apoptosis.
We investigated the biosorption characteristics of thallium (I) ions using dead biomass of Pseudomonas fluorescens strains as biosorbents. The biosorbents were characterized by Fourier transform infrared spectroscopy (FT-IR) and a scanning electron microscope (SEM). The effects of different environmental factors such as initial Tl concentration, initial solution pH, biomass dosage, and contact time were evaluated. The maximum adsorption capacity was found to be 93.76 mg/g at an optimum initial pH of 5.0, a contact time of 60 min, a biomass of 0.5 g/L, and an initial Tl concentration of 50 μg/mL. The biosorption process can be well defined by the Langmuir isotherm (R²= 0.9967). The biosorption kinetics were better described by the pseudo second-order model (R²= 0.9950) than the pseudo first-order one. The analysis of (FT-IR) indicates that the main functional groups responsible for adsorption of Tl (I) were hydroxyl, carboxyl, and amino groups. SEM analysis verifies an obvious surface morphology change of adsorbed biomass. The results presented in this study show that the Pseudomonas fluorescens could be an effective, low-cost, and environmentally friendly biosorbent for removing Tl (I) from aqueous solution.
Hylocereus undatus flower is commonly used as food or for medicinal purposes in south China. To study its antioxidant activity and mechanism we used antioxidant and chemical assays to compare two commercial samples from different locations (Shenjing, Qixing). The difference in antioxidant levels corresponded with differences in chemical content (including total phenolics, total flavonoids, kaempferol and quercetin) between Shenjing and Qixing. The antioxidant ability of H. undatus flower seems attributable to total phenolics (mainly total flavonoids). Kaempferol is one of the main bioactive components. H. undatus flower exerts its antioxidant effects through metal chelation and radical scavenging via hydrogen atom (H•) and electron (e) donation.
Histamine-containing enterochromaffin-like (ECL) cells are numerous in the gastric mucosa. They operate under the control of gastrin. ECL-cell tumors (gastric carcinoids) may arise as a consequence of sustained hypergastrinemia. For reasons unknown, such tumors have a female preponderance both in laboratory animals and humans. The present study consisted of four experiments exploring the possibility that gender-related factors might affect rat ECL cells. 1) A gender difference in terms of serum gastrin concentration and oxyntic mucosal histidine decarboxylase (HDC) activity appeared in Sprague-Dawley but not Wistar rats. Ultrastructural appearance of the ECL cells did not differ between genders. 2) During the different phases of the estrous cycle, the serum gastrin concentration, HDC activity and histamine concentration did not change. 3) During pregnancy, the serum gastrin concentration was suppressed, while it was increased during lactation. The HDC activity and the histamine concentration of the oxyntic mucosa were correlated with the levels of circulating gastrin. 4) Twelve-month treatment with estrogen-like agents, dieldrin and/or toxaphene (alone or in combination) was without any effect on the ECL cells neither in male nor in female rats. In conclusion, the ECL cells are under the control of gastrin, but probably not hormones that involve in the estrous cycle and pregnancy and lactation in rats. Possible gender-related factors behind the female preponderance of ECL-cell tumors remain unknown.
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