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Анализировали величину и причины потерь поросят от первородящих и многородящих свиноматок пород крупной белой польской и дюрок до 70-дневного возраста. Исследовательский материал составляли данные касающиеся 1147 павших поросят в период 1985 и 1986 гг. в Гибриднзационном центре Павловице. Число мертворожденных поросят было выше в пометах свиноматок породы дюрок. В период откармливания до 21-го дня жизни наблюдались более высокие потери поросят в пометах породы дюрок. Потери до 21-го дня жизни были выше в пометах первородящих свиноматок, вызванные нарушениями питательного тракта.
In the present paper we focused on the potential role of human lactoferrin for the intracellular replication rate of T. gondii BK tachyzoites following our earlier observation that Toxoplasma gondii was able to bind human lactoferrin but not serum transferrin. The study was performed in vitro on human CaCo-2 epithelial cells and mouse L929 fibroblasts. We found that the multiplication of the parasite was inhibited by lactoferrin in both cell lines used. However, the direct cytotoxic effect on the parasite and the host cells was not observed. The intracellular growth of T. gondii was not affected when tachyzoites or host cells were only pre-coated with human lactoferrin. The results suggest that lactoferrin does not influence parasites penetration into host cells but could trigger unknown antiparasitic mechanisms in the infected cells.
Macrophage-mediated early nonspecific immunological response is an important part of the immunity against intracellular parasite Toxoplasma gondii. The immunological functions of macrophages are closely connected with iron metabolism and acquiring of iron mainly from transferrin by the receptor-mediated endocytosis. The level of specific transferrin receptors can be modulated by different soluble exogenous and endogenous factors and also by microbial pathogens. The goal of our study was to determine the influence of T. gondii infection and toxoplasma lysate antigen (TLA) on the expression level of transferrin receptors (TfRs) on mouse macrophages and fibroblasts which can serve as host cells for the parasite replication. The level of TfRs was measured using CELISA assay. Strong down-regulation of the receptors level, started about 18 hours after infection of macrophages with a high number of freshly harvested tachyzoites T. gondii. Stimulation of the mouse cells with TLA antigen did not cause any changes in TfRs expression. In our studies we did not observe any differences in the TfRs level on mouse fibroblasts even after incubation with high concentrations of TLA antigen or inoculation with a high number of tachyzoites.
The parasitic protozoan Toxoplasma gondii is capable of altering intermediate host natural defensive behavior, which is believed to facilitate the parasite’s transmission in the environment. Despite extensive research on the subject, the exact mechanism behind the host manipulation remains obscure. However, key neurotransmitter levels are listed among possible contributing factors. Thus, the study was aimed at evaluating the monoaminergic activity in specified brain regions of T. gondii infected mice of both genders. The obtained results show that parasite invasion influences all tested monoamine systems and the observed changes depend on gender and time after infection. The parasiteinduced neurotransmission alterations were mostly pronounced during acute toxoplasmosis and they involved a decrease in noradrenergic system activity in females and its slight increase in some brain areas of males as well as a rise in serotonin and dopamine systems activity in males. These findings may contribute to a better understanding of T. gondii involvement in the host behavior control and in the occurrence of certain CNS disorders in humans. The study was supported by the Polish Ministry of Science and Higher Education (grant N N302 636340)
The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii infection since the protein was recognized by both mouse and human sera, 100% and 94.3%, respectively, rendering the full-length rMAG1 a prospective alternative for the polyvalent native antigen (TLA).
The aim of this study was to test the potential diagnostic usefulness of recombinant Toxoplasma gondii rhoptry antigens, ROP2 and ROP4, with respect to toxoplasmosis detection and infection phase distinction in laboratory mouce by determining specific serum IgM and IgG antibodies with the use of indirect ELISA technique. The mice antibody response to ROP antigens was significantly higher in the IgM than in the IgG class with the peak on the turn of acute and latent infection, whereas the response to recombinant SAG1 antigen, used as control, revealed preferential synthesis of IgG antibodies with the highest absorbance values measured during latent toxoplasmosis.
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