NGF (nerve growth factor) is involved not only in growth and survival of neurons but also promotes their age-dependent morphological changes (repair and remodeling) in normal life and during stress. This study aimed to investigate an infl uence of ages, on the changes of NGF immunoreactive (-ir) cells in the: amygdala, hippocampus and hypothalamus caused by acute (one-time for 15 min) or repeated (21 days for 15 min daily) exposition to open fi eld (OF) test. Each group of age consisted of experimental and control (non-stressed) Wistar male rats. To detected NGF-ir cells single immunofl uorescence staining was applied. Each control groups revealed many of NGF-ir neurons in the studied structures. Following OF acute stimulation, the number of NGF-ir cells in all the studied structures was higher in the three months old rats than that of control ones; the level of NGF-ir cells in the one year old rats was higher only in paraventricular nucleus of hypothalamus and in central nucleus of amygdala. In two years old rats no changes was observed in comparison with control animals. After OF repeated exposition, the level of NGF-ir cells was similar to that observed under acute one. These data demonstrated that the aging affected the level of NGF-ir neurons caused by acute and repeated OF stimulation in the structures of limbic system. Stress duration did not infl uence the level of NGF-ir neurons.
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