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Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

100%

Nowak-Stepniowska A., Wiktorska K., Malecki M., Romiszewska A., Padzik-Graczyk A.

Acta Biochimica Polonica

2011

tom 58

nr 4

Porphyrin photosensitizers tend to localize in mitochondria. The depolarization of mitochondrial membrane is one of the early stages of apoptosis and Laser Scanning Fluorescence Microscopy allows to determine changes in transmembrane mitochondrial potential under influence of PDT depending on the kind of photosensitizer (PP(Arg)2, Hp(Arg)2), the energy dose (5, 10, 30 and 50 J/cm2) and time periods (24 and 48 hours after irradiation) in the LNCaP (lymphonodal metastasis of prostate carcinoma, the androgen dependent cell line). Cyototoxicity induced by PP(Arg)2- and Hp(Arg)2-based PDT depending on energy dose and time after irradiation in prostate carcinoma is determined with MTT. Generally, it was shown that lower energy doses induce greater changes in transmembrane mitochondrial potential. Hp(Arg)2-based PDT was more effective causing greater mitochondrial membrane depolarization and cell viability decrease in comparison to PP(Arg)2-mediated PDT (in the case of maximal nontoxic photosensitizer doses used).

Cytotoxity of PP(Arg)2 - and PP(Ala)2(Arg)2 - based photodynamics therapy and early stage of apoptosis induction in human breast cancers in vitro

86%

Nowak-Stepniowska A., Wiktorska K., Malecki M., Milczarek M., Lubelska K., Padzik-Graczyk A.

Acta Biochimica Polonica

2012

tom 59

nr 4

Mitochondria are cell energetic centers where ATP is produced. They also play a very important role in the PDT as intracellular sites of photosensitizer localization. Photosensitizers gathering in mitochondria (like porphyrin derivatives used in this work) are more effective in tumor cell destruction. Moreover, it was assumed that di-amino acid substituents attached to porphyrin ring will strengthen the effectivity of interaction with membrane receptors of examined cells. MTT assay was performed to investigate the influence of PP(Arg)2 and PP(Ala)2(Arg)2-based PDT on breast cancer cell viability for 24 h, 48 h and 120 h after cell irradiation. Then the influence of PP(Ala)2(Arg)2- and PP(Arg)2-mediated PDT on early mitochondrial apoptosis induction via measurements of the transmembrane mitochondrial potential changes was examined. Results showed that lower energy doses and maximal nontoxic photosensitizer doses of PP(Ala)2(Arg)2 and PP(Arg)2 applied in PDT can imply apoptotic cell death. It was confirmed that modification of the protoporphyrin IX by attaching two alanine substituents raised the efficiency of photodynamic therapy.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Malecki M.

2 Nowak-Stepniowska A.

2 Padzik-Graczyk A.

2 Wiktorska K.

1 Lubelska K.

1 Milczarek M.

1 Romiszewska A.

1 2012

1 2011

Wyniki wyszukiwania

Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

Cytotoxity of PP(Arg)2 - and PP(Ala)2(Arg)2 - based photodynamics therapy and early stage of apoptosis induction in human breast cancers in vitro