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Soil erosion tends to occur with rainfall runoff, thus leading to grave soil and water loss. An increase of water content in soil caused by rain makes the loss of matrix suction and the decrease of shear strength obvious, and will promote soil erosion. The soil-water characteristic curve (SWCC) can be used to describe the relationship between the water content and the matrix suction in unsaturated soil. For this paper we studied the SWCCs of the granite residual soils in a collapsing erosion area in Jiangxi Province, China. A GEO-Experts pressure plate extractor was used to measure SWCCs for soils with different dry density, grain size, drying and wetting cycles, and lime content. The initial dry density has a significant impact on SWCC. With increasing dry density, the suction was decreased for the same water content. The larger the grain size, the greater the suction value for the same volumetric water content. Under the same suction, the volumetric water content decreases as the lime percentage increases and water stability improves. SWCCs of the drying and wetting cycle demonstrate the hysteresis phenomenon. The area of the hysteresis loop decreased with the increase of the dry density and drying and wetting cycle number. It also became small when the soils were mixed with lime. In this paper, the Van Genuchten model, the Fredlund and Xing model, and the Gardner model were used to fit the experimental data of SWCCs. The presented fitting parameters show that the residual sum of squares is less than 0.002. All the experimental data fit well to three models for SWCC. The results indicated that the simulated value of the Gardner model does provide best agreement with the measured value. These results will provide an important basis for the further study of the soil collapsing erosion process and soil cover design.
Impounding by the Three Gorges Project (TGP) changed the hydrological process and affected the ecosystem in the middle and lower reaches of the Yangtze River. Poyang Lake is closely linked with the Yangtze River. This paper presents a coupled numerical model designed for simulating the tempo-spatial variation of water quantity and quality in the lake’s water system that features complex river-lake interactions. According to the calculated results of a typical year (2000) under two scenario schemes, we evaluated TGP’s effects on the ecological environment of the lake. The results show that due to TGP impoundment, the hydraulic gradient at the lake outlet has increased and that the outflow fluctuates dynamically and regularly with the increase and decrease of the TGP released discharge. The lake water level falls in advance and the average reduction magnitude of the water level decreases progressively from north to south, showing a spatially uneven effect, which is 0.95 m, 0.70 m, 0.74 m, 0.35 m, and 0.80 m at Xingzi, Tangyin, Duchang, Kangshan, and Wucheng stations, respectively. The ammonia nitrogen output from the lake to the Yangtze River increases and the total ammonia nitrogen amount within the lake region decreases. The average ammonia nitrogen concentration within the lake region tends to increase slightly and the influence magnitude on the concentration is reduced from north to south. The wetland vegetation area fluctuates dynamically with the decline of the water level, and the increase of the wetland vegetation area ranges from 0.64 to 215.48 km2. As a result of the falling water levels, the bottomlands in the lake region are exposed relatively earlier and the continuous exposure days increase, which intensifies the problem that migrant birds do not have enough food. Meanwhile the number of days when the bottomland and wetland are submerged decrease, which will further accelerate the degradation succession of wetland vegetation.
In recent years, biochar, a new environmentally functional material, has received widespread attention as a soil amendment for its special structures and characteristics, such as improving soil texture and increasing crop yield. However, controversies still exist for the effects of biochar addition to soil nitrogen(N) cycles. This study focuses on the influences of 350ºC peanut biochar application on N mineralization in abandoned orchard soil during a 46-day incubation. The treatments contained control (CK), 1% biochar (BC), and 3% biochar (BC). Results showed that 350ºC peanut biochar increased soil pH and EC, but decreased soil urease activities significantly. Biochar decreased soil net-mineralized N significantly, and a higher biochar addition content resulted in lower soil mineralized N concentration. NO3–-N content accounted for more than 94% of soil inorganic N for all three treatments during the entire incubation time, while the presence of biochar did not change this characteristic. Conclusions indicated that the addition of 350ºC peanut biochar significantly inhibited orchard soil N mineralization in a short time by altering soil physical and chemical properties.
Studies have shown that the stress tolerance of cereal plants to osmotic or salinity stresses can be improved to varying degrees by the overexpression of an introduced betaine aldehyde dehydrogenase (BADH) gene. In the present study, the HvBADH1 gene from Hordeum vulgare L. var. nudum Hook. f., encoding a cytosolic BADH, was transferred into Triticum aestivum via traditional Agrobacterium tumefaciens-mediated transformation. Molecular methods, such as PCR, Southern blot analysis, and real-time quantitative RT-PCR were used to identify the successful integration and expression of the HvBADH1 transgene in genetically transformed wheat lines. To detect the efficacy of the HvBADH1 transgene in the transformants, some pivotal physiological indicators that reflected abiotic stress tolerance were measured in individual transgenic plant lines. These indicators included intracellular K⁺ and Na⁺ contents or K⁺/Na⁺ ratio, relative conductivity, and malondialdehyde and glycine betaine (GB) concentrations in cells. The results revealed that all the tested transgenic lines could significantly increase the recruitments of K⁺ in their cytosol than the wild-type seedlings. Similarly, 11.59- to 21.82-fold greater accumulation of GB, 2.11–2.56 times higher calli relative growth rates, and 26.2–29.1% seedling survival rates were found in transgenic lines under 150 mM NaCl stressed conditions. Our results demonstrated that by overexpressing the HvBADH1 transgene in genetically transformed wheat, the overall salt tolerance of the target plants was significantly increased, and the damaging effects of high salinity were significantly reduced.
The objective of this research was to investigate the catalytic role of nitrogen-doped carbon nanotubes (NCNTs) in a bioreactor that contained Acidithiobacillus ferrooxidans (A. ferrooxidans) and waste printed circuit boards (WPCB). Factors that could refl ect the results, such as pH, oxidation-reduction potential (ORP), Fe²⁺, and leaching rate of copper were detected and calculated in the bioleaching process. The results demonstrated that a certain amount of NCNTs had good compatibility with A. ferrooxidans, and it had the best consistency when added to 0.04g NCNTs in 50 ml solution. NCNTs could promote nearly 10% of copper leaching rate from WPCB by A. ferrooxidans compared with the blank group. When the NCNTs were treated by acid solution, its ability to promote the leaching rate of copper had a certain degree of decline (but not obvious), and it was about 2% lower than the group in which the NCNTs were not treated with acid. These phenomena could expand the application of NCNTs, and these could provide a basis for the next industrial application of A. ferrooxidans.
The effect of introduction of the Zephyranthes grandiflora agglutinin gene (zga) to tobacco on its anti-pest ability for peach-potato aphids was investigated. PCR analysis confirmed that the zga gene was integrated into the plant genome. The results from semi-quantitative RT-PCR and real-time PCR assays revealed that the zga gene was expressed at various levels in the transgenic plants. A bioassay with aphids indicated that transgenic plants conferred enhanced resistance to aphids. Compared with the controls, the average number of aphids fed with transgenic plants during a 20-day assay evidently decreased by 70.4% in leaf disc bioassay and 77.9% in whole plant bioassay. The average number of nymph was significantly reduced by 36.4% on zga-expressing plants in leaf disc bioassay and 35.6% in whole plant bioassay. The report indicated that the introduction of zga gene to tobacco plants is a useful method to improve its anti-pest ability for aphids.
Thallium (Tl) is a typical trace metal of extreme high toxicity. As a concomitant element, Tl is widely found in various sulfide minerals and K-containing rock minerals. The outburst of Tl pollution in drinking water sources of the northern branch of the Pearl River in China as reported in 2010 has greatly aroused public concerns about Tl pollution in China. Apart from typical sources of Tl pollution such as Pb and Zn smelting and the mining and utilization of Tl-containing pyrite ores, the steel-making industry was discovered a new significant source that contributed to this Tl pollution incidence. Thallium contents in raw materials, fly ash and wastewater collected from a typical steel-making enterprise were determined by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that Tl contents (0.02-1.03 mg/kg) are generally low in the raw materials, while fly ash samples have generally enriched Tl levels (1.31-6.45 mg/kg). Wastewater obtained from the dedusting process of the sintering furnace also exhibited excessive Tl levels (574-2130 μg/L). All these results suggested a possible release and gasification of Tl compounds from the raw materials under high temperatures (>800ºC) during the sintering processes, which were then accumulated in the flue gas and fly ash and washed into the wastewater by wet dedusting. Lime precipitation method is not effective for removing Tl from wastewater, since Tl mostly is present as dissolved Tl⁺ in the water. The study initiated a preliminary design of a fast and effective treatment method for Tl removal from Tl-containing industrial wastewater by using a deep oxidation system.
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