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Twenty Chios newborn ewe lambs were used. Ten lambs were fed ad libitum colostrum and dam milk (group 1). The other ten lambs were separated from their dams immediately after birth and were not allowed to suckle normally, but were fed commercial cow's milk with a feeding bottle (group 2). When they were one year old, M. haemolytica serotype Al (10⁹ germs) was inoculated intratracheally to each ewe. Serum vitamin E and malondialdehyde (MDA) concentrations were determined in all the sheep before inoculation, and on days 1, 10, and 22 after inoculation, in addition to day 7 after antibiotic treatment. Serum vitamin E concentrations significantly decreased on day 1 after bacterial inoculation in group 1, rose on day 10 and continued to rise on day 22 after inoculation. The concentrations of the compounds did not significantly differ from the values observed on day 22 after antibiotic administration (on day 7 after treatment). Vitamin E concentration significantly decreased on day 1 after inoculation in group 2, and significantly rose on day 10 and then remained constant. Significant differences were observed between groups 1 and 2 before inoculation (P≤0.05). While serum vitamin E concentration of group 1 was higher than group 2 before inoculation, the concentrations in group 2 were significantly higher than in group 1 on days 10 and 22 after inoculation. Serum MDA concentrations gradually significantly increased in groups 1 and 2 after bacterial inoculation (P≤0.05), and then decreased to the pre-inoculation values after antibiotic treatment. No significant difference was observed between group 1 and group 2 on any sampling day. In conclusion, M. haemolytica infection induced lipid peroxidation associated with a rapid consumption of the antioxidant vitamin E (day 1), following by mobilisation of liver vitamin stores, probably more intense in ewes fed artificially after birth. The similar serum MDA concentrations in the two groups suggests that, a resistance against an infection can develop in sheep fed with artificially cow's milk within one year, but the effects of repeated infections on such animals still remains to be explored.
The objective of the study was to investigate the effect of an energy restriction on serum cortisol and its faecal metabolite (11,17-dioxoandrostan) in Chios ewes in late gestation. Twenty pregnant and ten non-pregnant out of 38 randomly selected ewes were detected with ultrasound and were separated into three groups (n=10) on day 105 after mating: normal energy fed pregnant group (NE), restricted energy fed pregnant group (RE), and non-pregnant group (NP). Blood samples were taken on days 117, 130, and 144 of pregnancy and faecal samples were taken twice a week starting on day 106 of pregnancy from both pregnant and non-pregnant ewes. After extraction of serum and faecal samples, concentrations of cortisol and 11,17-dioxoandrostan (11,17- DOA) were measured. A significant decrease in RE was determined only on day 144 of pregnancy (P≤0.05). Between the two pregnant groups (NE and RE), a significant difference was found only on day 144; at this period, RE had lower serum concentrations. RE had significantly lower 11,17-DOA concentrations in late pregnancy than NE. In pregnant ewes, the levels decreased in a late gestational period. The present data shows that in ewes fed 14.47% crude protein and 8.82 MJ /kg of metabolic energy in late pregnancy, pregnancy toxaemia does not occur. We suggest that the determination of Cortisol metabolites in faeces is more objective and safe than that of serum Cortisol concentrations.
Ghrelin is a peptide hormone which plays important role in maintaining growth hormone release and energy homeostasis in vertebrates. Spirulina platensis (SP) has antioxidant and hypolipidemic effects due to its ingredients. In this study we aimed to investigate the effects of SP on the testicular structure and relation between ghrelin and testosterone in the testis of rats fed a high fat diet (HFD). Sixty four young adult male rats were used and divided to 8 equal groups. Experimental groups received addition of 10% cholesterol (CHL), 43% hydrogenated vegetable oil (HVO) and 3% SP alone or in combination to basal diet while the control group received only basal diet. Serum ghrelin and testosterone levels were measured with ELISA. Receptors for ghrelin and androgen were detected with immunohistochemistry. For histomorphometric investigation, tubulus seminiferus, intertubular area, tubulus seminiferus lumen, Leydig cell nucleus, Sertoli cell nucleus, germ cell nucleus, spermatocyte nucleus and elongated spermatid volume densities were determined stereologically. Serum ghrelin level was increased especially in HVO and CHL combination group compared to the control while serum ghrelin levels were close to control levels in SP-received groups. Ghrelin receptor level was increased in tubulus seminiferus with HVO+CHL administration but this effect was, however, limited in HVO+CHL and SP challenged groups. HVO+CHL administration caused a significant decrease in Leydig cell nucleus volume density, as well as in all SP-received groups, compared to the control. Significantly increased spermatocyte nucleus volume density in cholesterol-receiving groups was decreased to control level with SP alone and its combinations.
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