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In a greenhouse experiment using sand columns, sorghum and pearl millet were grown and spiked with metal solutions of Cu, Cd, and Cr in two concentrations. The chelating agent EDTA was applied to one-month-old plants and metal mobilization was observed through uptake by the plants or leaching through the columns during a period of one month. Growth was much better in pearl millet than sorghum under metal stress. Metal uptake was significantly higher in sorghum and was in the order root>shoot>leaves in both the plants. Metals were differentially mobilized in the order of Cu>Cr>Cd as shown by plant uptake and leaching through the columns. The root to shoot translocation of Cd was significantly improved after EDTA application, and to some extent for Cu but not for Cr. The leaching of metals was the maximum in the second week of application and was almost negligible by the fourth week conforming to the amount of EDTA detected in the leachate. Leaching of Cr differed in the maximum amount at the time of chelant application, while Cu and Cd showed maximum leaching after 7 days. As compared to sorghum, pearl millet was better at controlling leaching because of its extensive root system.
Wild vegetables can contribute to people’s food security and health. In northwest Pakistan, almost 40% of the households are classified as food insecure, due to increasing population and natural and man-made catastrophes. There is an urgent need to get an overview of still existing practices of wild vegetable use and to incorporate such knowledge into agricultural policies. The present study, therefore, aims to collect and analyze information on the most widely and commonly used wild vegetables in northwest Pakistan. Semistructured interviews were performed with 126 informants (26–78-year old) from all 24 districts of the Khyber Pakhtunkhwa Province. Information on culinary and medicinal use, cooking methods, and growing and harvest season were collected. A rating scale was used to get the opinion on quality, abundance, and use frequency of wild vegetables. Information on trading was gathered on local and regional markets. Transect walks were done with key informants for specimen collection. A total of 25 wild vegetable species (21 genera, 13 families) were documented. Most of them are herbs (22 spp.). One third of the species needs processing or detoxification prior to cooking. Taste and food quality as perceived by the local people are the main driver for use frequency and commercialization. Length of availability varies from 2 to 7 months with Rumex dentatus and Vicia faba also available during the winter season. Overall, 21 spp. are also used medicinally, mainly for gastrointestinal diseases. Leaves are the preferred plant part for both vegetables and medicines. Nine species are sold in markets, Bauhinia variegata and Caralluma tuberculata throughout the whole province. Local communities in northwest Pakistan have a broad knowledge of local plant use, especially on wild vegetables and their adequate processing. The present study suggests that the most commonly used wild vegetables should be promoted to reinforce food safety and most of them may also be commercialized. Conservation priority has to be given to the commercially harvested Caralluma tuberculata, which is presently overexploited.
An in-vitro study was conducted to estimate the pesticidal residues in five types of fruits; melon, guava, orange, peach, and mango. Organochlorine, organophosphate, carbamates, and pyrethroids, along with other insecticidal residues, were detected. The collection of samples was carried out from farmland located in the indigenous soil of Faisalabad and Toba Tek Singh. The residual pesticides were extracted by single-phase extraction and then purified by the gel permeation chromatographic technique. The extracted residues were isolated and quantified by HPTLC method. The detected residues were in the range of 0.1-0.3 mg kg⁻¹. The study demonstrated a high risk of ongoing contamination posed by uncontrolled applications of pesticides, which are needed to be overcome on the lines of sustainable management measures.
This work illustrates the proximate analysis on three types of coal samples obtained from Khushab mines in Pakistan. Analysis of the coal matrix was carried out to determine volatile matter, ash contents, moisture, fixed carbon contents, calorific values, and sulfur contents as per standards of American Society for Testing and Materials (ASTM). The results of proximate analysis show 30.46% fixed carbon content, 28.08% ash content, 6.96% total moisture content, and 34.5% volatile matter within sample A, whereas 40.56% volatile matter, 8.03% moisture content, 8.32% ash and 30.46% fixed carbon content was found in sample B. Sample C contained 15.44% volatile matter, 9.16% moisture content, 40.64% ash content, and 37.5% fixed carbon content, respectively. Sample B has higher calorific value and sulphur contents in comparison to A and C. These coal samples are of good quality from an industrial prospective, but they need to be desulphurized prior to industrial use for the sake of the environment.
This work focuses on mapping landscape connectivity by making use of a subdivision of a Harary graph through super edge antimagic total labeling. This study employs a Harary graph by inserting h vertices in each edge, where h = 2n, n ≥ 1 using the super (a, 2) edge antimagic total labeling and labeling the vertices and edges by taking the difference of arithmetic progression as 2 i.e. d = 2. We divided this paper into two parts. In first part, when the order of the subdivided harary graphs p varies then the distance t will remain the same, while in the other part, when the order p varies then distance t will also vary.
Vegetables are considered a rich source of such antioxidants as phenolics, carotenoids, flavonoids, and vitamins, which are frequently added to stop the process of oxidation in processed food and biological systems. This work aimed to investigate the antioxidant potential of vegetable waste (garlic, onion, and cauliflower). The extracts of vegetable waste were prepared by two solvents – 80% methanol and 80% ethanol – and were appraised for their antioxidant potential. Total phenolic contents (TPC) of these vegetable waste extracts were in the range of 2.23-16.12 mg Gallic acid equivalents/gram (GAE/g) of dry weight (DW), while total flavonoids were in the order of 0.24-2.13 mg catechin equivalent/gram (CE/g) of DW. Maximum inhibition capacity and maximum scavenging activity was displayed by ethanolic extract of onion waste. The onion waste extract compared to others also showed high reducing power (1.27) as it had higher intensity of color. This analysis declares onion waste with maximum value of TPC, total flavonoids content (TFC), 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), and the percentage of age inhibition.
In this study we screened Viburnum grandiflorum for bioactive secondary metabolites and biological activity. Secondary metabolites were detected by phytochemical tests, and biological activity was confirmed through antimicrobial and anti-oxidant assays. Phytochemical screening (alkaloidal, tannins, terpenoids, flavonoids, anthraquinones, and glycosides) was performed with methanol, and aqueous and ethyl acetate extracts. Antibacterial activity against four bacterial strains — staphylococcus auries, Escherichia Coli, Bacillus subtillus, and salmonella typhi – were measured. Methanolic extract showed maximum inhibitory activity with diameter of zone of inhibition (11.66 mm), followed by n-hexane extract (9.33 mm) and then ethyl acetate extract. Four different fungi (Penicillium chrysogenum, Aspergillus flavus, Rhodotorula mucilaginosa, and Stachybotrys chartarum) were also tested against plant stem extract using different solvents. Dimethyl sulfoxide extract showed a maximum zone of inhibition at 20 mg/ml. Anti-oxidant activity of stem extract of Viburnum grandiflorum was evaluated by 1, 1-diphenyl- 2-picryl-hydrazyl (DPPH). Then we measured absorbance, and percentage activity at each concentration was found for three solvent extracts to get Ic50 values. These data support Viburnum grandiflorum as having enough potential to be used safely as an antimicrobial drug.
This study is intended to scrutinize the consequence of extraction conditions of Lawsonia Inermis (henna) leaves on the color strength values of dyed fabric. Extraction pH, M:L ratio, and time were optimized using central composite design (CCD) under response surface methodology (RSM). We investigated how the maximum colour strength values were obtained at pH 6.64 in about 78 minutes extraction with an M:L ratio of 1:44. ANOVA results showed that all the extraction parameters significantly affect the colour strength values of dyed samples. 99% variation in the response was indicated in the 2nd-order regression equation for K/S. The fastness properties with respect to light, washing, and rubbing were quite satisfactory of dyed samples under optimized extraction conditions.
This study focuses on evaluating total phenolic contents (TPC) in Taraxacum officinale (L.), a member of the family Asteraceae (compositae). The TPC were estimated by Folin-Ciocalteu’s reagent and gallic acid was taken as standard. The amount of phenolics was communicated as gallic acid equivalent (GAE). The TPC varied from 41.47 mg/g to 691.6 mg/g in the Taraxacum officinale (L.) extracts. The maximum phenolic contents were found in hydro-alcoholic extract (691.6 mg/g GAE) in comparison with aqueous extract. These extracts have a significant role as antibacterial and antimicrobial agents.
An enormous amount of waste material (sludge) generated from paper and pulp industries is either dumped or incinerated. However, this pulp is an enriched source of macronutrients and can be employed on agricultural land to improve its fertility. The current study was therefore performed to analyze phosphorus (P) and calcium (Ca) by flame photometer, nitrogen (N) by Kjeldahl method, and magnesium (Mg) by back titration from sludge. The application of sludge significantly increases macronutrients in the plants lady finger (Abelmoschus esculentus) and garden mint (Mentha sachalinensis).We applied primary (initial husk treatment) and secondary (after primary treatment) sludge to observe the growth of Abelmoschus esculentus and Mentha sachalinensis. However, primary sludge showed the highest concentration of nutrients, i.e., 96.5% P, 99.6% N, 86% Ca, and 0.008% Mg. The application of paper and pulp waste to the soil indicates that these bio-solids are good for soil fertility, biomass production, and plant growth; however, they are slightly less efficient than synthetic fertilizers. However, the synthetic fertilizers are probable candidates for holding heavy metals, so these bio-solids can be considered a better source for soil fertility.
This study looks at using partially purified peroxidase extracted from peels of sweet lime (Citrus limetta) for decolorizing textile industry effluent. The ideal pH and thermal conditions of the enzyme were 7 and 35ºC. The Km and Vmax for guaiacol were 0.66 mM and 6666 μmol/mL/min, respectively. We found that sweet lime peroxidase was very effective in decolorizing textile industry effluent. Almost complete decolorization (>99 %) of effluent was attained at a pH of 5.0, temperature of 55ºC, H₂O₂ concentration of 2 mM, and enzyme dose of 40 U/mL within 60 minutes of incubation. The effluent was also analysed in terms of physicochemical parameters before and after treatment with sweet lime peroxidase. The reduction in toxicity after the enzymatic treatment was evidenced by chemical oxygen demand (COD) and total suspended solids (TSS) values.
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