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The aim of the study was to evaluate the effect of two different methods of partial prostatectomy, to evaluate the influence of 96° ethanol injection on hyperplastic prostate and to compare the efficacy of ethanol injection with the efficacy of surgical treatment of the prostate. The study was performed in the years 20012003 in the surgical department of Dr. L. Kriauèeliûnas Small Animal Clinic. The surgical treatment (prostatectomy) was performed on 8 dogs using 2 methods: fillet and subcapsular. We compared the effectiveness and postoperative hystological evaluation of bioptats one and two weeks after surgery. We have performed ethanol injection on 5 dogs and evaluated the histological feature of prostatic tissues after injection to measure the effect of ethanol. The fillet method is technically simpler, shorter and the tissues of the prostate heal more quickly. Ethanol injection induces multi-focular coagulative necrosis, followed by fibrosis and atrophy of the prostate. This way of treatment is simpler and easier because there are no sections through prostate tissues. To avoid a section of the abdominal wall, a transurethral catheter can be used for the ethanol injection.
The aim of this study was to investigate the effect of electromagnetic fields on BALB/c strain mice on their health, body weight, behavior characteristics, hematological parameters and histopathological findings in the brain. The mice of the experimental groups were exposed to electromagnetic waves by using Nokia 230 and Samsung 19300 Galaxy S III mobile phones situated at 2 cm from the cages. In the present study, it can be concluded that the exposure of mice to mobile phone radiation had an effect on the structure of the brain, behavior and body weight. The waves of mobile phones increased activity characteristics and changed some behavioral categories of mice and also decreased their body weight. Histopathological examination revealed mild edema of neutrophils and degeneration of some neurons and glial cells in the brains of experimental mice. The results of the present study showed that a using mobile phone had an influence on in vivo systems.
The purpose of this study was to broaden the basic knowledge of corneal morphological structures in pigs, cows and rabbits and to find morphological and histological differences in the constituent corneal structures. After slaughter, corneal samples of 24 animals, including cows, pigs and rabbits, were investigated. Forty-eight eyeballs (n = 48) of 8 pigs, 8 cows and 8 rabbits were used for this study. Central corneal thickness (CCT) values in cows were higher than those in pigs (P < 0.001) and rabbits. In rabbits, CCT, as a percentage of the eye axis length, was 55.03% greater than in cows and 40.55% greater than in pigs (P < 0.001). The thickness of substantia propria (stroma) depended on the species and accounted for more than 80% of the thickness of all layers of the cornea. The thickness of the anterior epithelium amounted to 15.81% of the thickness of all layers of the cornea in cows, 11.79% in pigs, and 8.48% in rabbits (P < 0.001). The data analysis showed that the caudal limiting membrane + endothelium was the thinnest layer of the cornea, with thickness ranging from 0.88% to 2.40% of the total thickness of all layers of the cornea in the three species under study (P < 0.001). A statistically significant negative correlation was determined between the anterior epithelium and substantia propria in corneas of cows, pigs and rabbits (P < 0.001). This paper describes the morphometric parameters of the cornea in pigs, cows and rabbits. These parameters will be of use to scientists using animal models in vision science research.
The aim of this study was to investigate the influence of long-term building construction noise from refurbishment, which including vibration, on some physiological parameters and histopathological changes of organs of Wistar rats. Twenty 12 month old female rats were divided into two groups: rats group I (n = 10) were exposed to long-term construction noise and rats group II (n = 10) were kept under normal noise level. Study results revealed that long-term construction noise from building refurbishment has an influence on body weight, haematological and some serum biochemical parameters affects caecal microbiota, and causes histopathological changes in the organs of adult female Wistar rats. It was noticed that rats in group I exihibited significantly higher mean values for total protein, albumin and lower values for glucose, AST, ALT, blood urea nitrogen, haematological and caecal microbiota parameters than rats in group II. The most common pathologies were determined in the kidney, liver and lungs. Other observed pathologies were lymphadenopathy, catarrhal inflammation of the intestines, spleen hyperplasia and mammary gland adenofibroma. Single cases were subcutaneous fibroma in the thoracic region, abortus with uterine inflammation and thymus hyperplasia with formation of cysts were found.
Many researchers have pointed out that the intake of omega-3 and -6 fatty acids is beneficial to animal health and reproductive capacity, but the effect of fatty acids on the histomorphology of ram testes is still unknown. The aim of this study was to investigate the effect of linseed oil containing polyunsaturated fatty acids on the morphology and histomorphometric characteristics of ram testis and epididymis. Testes of 12 Lithuanian local coarse wool rams were investigated histomorphologically and histomorphometrically in the present work. The rams were divided into two groups, an experimental group and a control group following, the principle of analogues – the same origin, age, weight, and condition score. The experimental group (n = 6) received 2% linseed oil sediments while the control group (n = 6) received 1.2% sunflower oil for 9 months. The diameter of testicular tubules and the height of the germinal epithelium, the spermatogenic index and the number of Leydig cells were higher in the experimental group than those in the control group. In addition, the number of degenerated tubules was not significantly lower in the group that received 2% linseed oil sediments. Still, the height of the epididymal epithelium was lower than that in the control group.
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