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The effect of interval vs. continuous exercise on plasma leptin and ghrelin concentration in young Standardbred horses was studied. The experiment was conducted on 27 trotters, in the age between 2 and 3 years. They were divided into two groups according to the type of exercise. Blood samples were collected through jugular venipuncture in the following experimental conditions: at rest, immediately after exercise and 30 minutes after the end of the effort. Plasma leptin and ghrelin concentrations were determined using RIA tests. The continuous exercise induced an increase in plasma leptin concentration whereas the interval type of exercise did not influence the level of this hormone (3.47 ± 0.78 vs. 4.07 ± 0.94 and 2.31 ± 0.15 vs. 2.36 ± 0.21 ng/mL, respectively). The plasma ghrelin concentration measured after the continuous exercise, significantly increased (720 ± 27.4 vs. 814 ± 13.8; p ≤ 0.05) whereas concentration of this hormone assessed after the interval exercise, significantly dropped (982 ± 56.5 vs. 842 ± 35.6 pg/mL; p ≤ 0.05). The changes in plasma ghrelin concentration measured after the end of the effort correlated inversely with blood lactic acid concentration. In conclusion, the obtained results showed that medium-intensive type of exercise, such as trot, interval or continuous, slightly affected plasma leptin level but significantly affected plasma ghrelin concentration in young Standardbred trotters.
The aim of this study was to determine the effect of dietary supplementation with α-ketoglutarate (AKG) sodium salt on growth rate in relation to bone collagen formation during the first 70 d of postnatal life in piglets. The results show that dietary AKG supplementation increased body weight of the experimental piglets in comparison to the controls, especially between 21st and 56th d of life (P≤0.01). Moreover, the area of collagen trabeculae slightly increased in experimental age sub-groups and reached the highest differences between 14th (P≤0.01) and 70th d of piglets life (P≤0.001). In contrast, the highest values for the number of collagen trabeculae were observed in piglets at 3rd d of age, regardless of treatment group. The positive effect of AKG supplementation on the number of collagen trabeculae was found between 3 and 35th d of life, with statistical confirmation at days 14, 35, and 56 (P≤0.01). The data-lines of the bone strain showed similar course during the whole experimental period, except 56th d of life, when the experimental piglets reached statistically significant, higher values in comparison to the controls (P≤0.05). Similarly, the blood plasma osteocalcin reached the highest concentration in experimental sub-groups from 21st d oflife in comparison to the controls, with statistical significance at the age of 56 (P≤0.05). These data indicate that dietary AKG supplementation effectively stimulated collagen synthesis in young growing piglets, both before and after weaning.
The aim of this study was to compare the effects of types of training: interval versus endurance on heart rates (HR) and the levels of chosen blood parameters in 28 Standard bred trotters, 2-3-years-old, trained for racing. The horses were divided into groups according to type of work. Three jugular venous blood samples were taken from each horse: at rest, just after the end of the training session and after 30 min rest. Hemoglobin (Hb) and lactic acid (LA) blood concentrations were determined as well as the plasma levels of glucose (Glc), glycerol, uric acid (UA), total plasma proteins and the activity of lactate dehydrogenase (LDH). Speed and HR during the training sessions were recorded by Polar speed and heart-rate telemeters. The interval training induced the increase in blood LA and Hb levels and plasma UA concentration directly proportional to the speed of trotting. A significant rise in the plasma level of glycerol determined immediately after exercise and 30 min later were observed during the intensity interval training session. These changes correlated positively with blood LA level and plasma Glc and correlated negatively with total plasma proteins concentration. The determination of plasma glycerol after exercise in trotters can be a good biochemical marker for assessing the process of interval training.
Insulin-like growth factor I (IGF-I) is a polipeptyde hormone produced mainly by the liver in response to the endocrine growth hormone stimulus, but it is also secreted by multiple tissues for autocrine or paracrine purposes. IGF-I represents one of the most important growth regulators, playing a central role in fetal and neonatal growth. However, the role of IGF-I in the reproductive physiology of horses is still little known. Therefore, the aims of this work were 1) to evaluate the IGF-I serum concentration in mares during the first 4 days after parturition and in their newborn foals during the first 4 days of life, and 2) to determine whether the IGF-I concentration may be influenced by the type of parturition. Two groups of subjects were examined: 7 healthy mares and their foals born by spontaneous parturition (group F), and 10 healthy mares and their foals born by non-spontaneous parturition, requiring medical assistance (group P). From each animal, the first blood samples were collected within 30 min of birth, and then, daily, during 4 days after parturition. The samples were collected once a day at 8.00 a.m. IGF-I was analyzed by the radioimmunoassay method with the IGF-RIA-CT kit (BioSource, Belgium). The results revealed that the mares from the group P had a statistically significantly higher concentration of IGF-I compared with the mares from the group F (90.9 ± 7.02 vs. 40.9 ± 5.94 ng/ml, respectively, p ≤ 0.01). Similarly, the statistically higher values of this factor were found in the foals from the group P than in those from the group F (130 ± 8.59 vs. 83.1 ± 6.56 ng/ml, respectively, p ≤ 0.01). Furthermore, a positive correlation was found between the concentration of IGF-I in the blood serum of mares and their foals on the first day of the study (r = 0.50, p ≤ 0.05). In summary, disturbances in the course of parturition did not have a negative impact on the IGF-I level in either mares or their newborn foals.
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