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1

Wartosc diagnostyczna antygenu Brucella ovis do OWD

100%
Iwaniak W.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 07
922-925
2

Zapalenie najadrzy u trykow wywolane przez Brucella ovis

63%
Iwaniak W., Pilaszek J.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 03
181-184
3

Bruceloza swin

51%
Szulowski K., Pilaszek J., Iwaniak W.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 04
283-286
4

Zwalczanie brucelozy bydla w Polsce i Unii Europejskiej

51%
Pilaszek J., Szulowski K., Iwaniak W.
Magazyn Weterynaryjny
|
2003
|
tom 12
|
nr 07-08
39-42
5

Comparison of PCR-based AMOS, Bruce-ladder, and MLVA assays for typing of Brucella species

51%
Weiner M., Iwaniak W., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
The aim of the study was the comparison between three PCR assays: AMOS, Bruce-ladder, and MLVA for typing Brucella reference strains representing different species. The B. abortus bv1, 2, 3, 4, 5, 6, and 9, B. melitensis bv1, 2, and 3, B. suis bv1, 2, 3, 4, and 5, B. ovis, B. canis, and B. neotomae strains were used. It was found that AMOS PCR could correctly identify only B. abortus bv1, 2, and 4, B. suis bv1, three biovars of B. melitensis (bv1, bv2, and bv3), and B. ovis. The remaining reference strains did not generated predicted amplicons or generated unspecific bands. In contrast to AMOS PCR, Bruce-ladder PCR was able to detect DNA from B. canis, B. neotomae, B. abortus bv3, 5, 6, and 9, and B. suis bv2, 3, 4, and 5. MLVA technique correctly recognised the species among B. aborus, B. melitensis, B. ovis, and B. neotomae using VNTR6, VNTR8, VNTR11, VNTR12, VNTR42, VNTR43, VNTR 45, and VNTR55 except the B. abortus bv5, which generated the same VNTR profile as B. abortus bv9. Moreover, MLVA did not differentiate B. canis from B. suis bv4. Only Bruce-ladder correctly identified the species of all tested Brucella strains and could be a useful tool for the rapid identification of species of Brucella strains.
6

Sytuacja epidemiologiczna brucelozy zwierzat w Polsce

51%
Pilaszek J., Szulowski K., Iwaniak W.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 06
363-366
The article presents the results of serological surveys of brucellosis conducted in 1998 on cattle, pigs, sheep, goats, hares, wild boars and dogs. No positive sero-reagents among the pig population were ascertained. The rate of positive results in cattle was established as 0.00098. When B. abortus antigen was used, all serum samples from sheep and goats reacted negatively. When B. ovis antigen was used, 0.91% of sheep sera were positive. The presence of anti-Brucella antibodies was demonstrated in hares, wild boars and dogs.
7

Verification of Sera from Cattle and Pigs for Brucellosis with Fluorescence Polarisation Assay

51%
Weiner M., Iwaniak W., Szulowski K., Szymajda M.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 2
Fluorescence polarisation assay (FPA) was evaluated as a potential tool improving specificity of serological diagnosis of brucellosis in cattle and pigs. The evaluation was performed by comparing the results of FPA with the results of rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test, and indirect ELISA when problematic sera, regarded as false positive, were tested. Four hundred and seventy-five sera, including 201 porcine and 274 bovine samples, reacting positively in at least one classical serological assay were used. Only six bovine sera were FPA positive (two positive in SAT and RB I and four positive in SAT only). Different situation was observed when porcine sera were examined. Out of 201 sera, 109 were also positive in FPA The studies confirmed that in cattle FPA enables to reduce highly the number of false positive reactions for brucellosis. On the other hand, in pigs, the method is a little more specific in comparison to other methods applied.
8

Occurrence of Yersinia enterocolitica O:9 in feces from cows seropositive for brucellosis

51%
Szulowski K., Weiner M., Iwaniak W.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 01
The diagnosis of brucellosis is mainly based on serological tests. All animals classified as serologically positive are obligatorily slaughtered and subjected to bacteriological examination. B. abortus has not been reported in Poland since the eradication of bovine brucellosis in 1980. On the other hand, B. suis biovar 2 is sporadically isolated from cattle. In accordance with the instructions of the Chief Veterinary Officer, samples of feces from animals slaughtered following positive serological results for brucellosis have been examined for the presence of Yersinia enterocolitica O:9 since 2011. Because of the similarity of its O-polysaccharide component of S-LPS with that of Brucella, this microorganism is considered to be a major contributory factor of false positive serological reactions (FPSR). The paper presents the results of the bacteriological and molecular examination of feces from 26 cows seropositive for brucellosis and 30 healthy cows, negative for brucellosis, for the presence of Y. enterocolitica O:9. Y. enterocolitica O:9 was found in 7 of the positive cows, whereas all samples from cows negative for brucellosis were free from this bacteria. These results indicate that Y. enterocolitica O:9 may be responsible for some of the positive results for brucellosis in cattle.
9

Genotypic markers of Yersinia enterocolitica O:9 isolated from cows positive in serological examination for bovine brucellosis

51%
Weiner M., Szulowski K., Iwaniak W., Zlotnicka J.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The aim of the study was to perform a molecular investigations for the presence of pathogenicity genotypie markers of Y. enterocolitica 0:9 isolated from cattle, in which initially positive serological reactions for brucellosis were observed. Almost all isolates were αil-, ystA- and myfA-positive (n=19). On the other hand, one isolate, which harboured plasmid encoding gene yadA was αil-, ystA- and myfA-negative. The plasmid encoding yadA marker was present in half of the isolates tested. None of the examined isolates was ystß-positive. The results of the investigations revealed that the Y. enterocolitica O:9 isolates, related to false positive serological results for brucellosis, may be also potentially pathogenic for humans, due to the presence of chromosomal and plasmid- encoded molecular markers.
10

Bruceloza psow

51%
Iwaniak W., Pilaszek J., Szulowski K.
Medycyna Weterynaryjna
|
1999
|
tom 55
|
nr 11
718-722
11

Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitica O:9

51%
Weiner M., Zlotnicka J., Iwaniak W., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
The aim of the study was to develop a multiplex PCR, which allows an identification of the universal 16S rRNA Brucella sp. marker and amplification of the perosamine synthetase (per) gene, specific for cross-reacting Yersinia enterocolitica 0:9 only. The PCR analysis of the DNA extracted from all Brucella reference strains used in the investigations revealed the presence of the 16S rRNA gene, generating the amplicon of 905 bp size. Parallely, the examination of the DNA from Y. enterocolitica belonging to 0:9 serotype showed the presence of predicted amplicon of 312 bp typical to 0:9 serotype only. The sensitivity of the developed assay was determined with lymph tissue samples inoculated with B. abortus bvl and Y. enterocolitica 0:9 strains. The PCR analysis of the DNA extracted from lymph tissue revealed the presence of the predicted gene, when the 10⁶-10² CFU/g of the bacteria was added to the lymph tissue. The mPCR developed with direct extraction of DNA from lymph tissue can be a useful tool for the differentiation of infections caused by Brucella and cross-reacting Y enterocolitica 0:9.
12

Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay

51%
Weiner M., Iwaniak W., Zlotnicka J., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The aim of this study was the application of fluorescence polarisation assay (FPA) for the examination of bovine sera and comparison of the results of the assay with the results of Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), and ELISA. Six hundred thirty-five sera from cattle, including 300 sera from healthy animals, 32 sera from animals regarded as serologically positive for brucellosis and culled, and 303 sera originated from confirmatory investigations were used. All sera originating from healthy animals, negative in ELISA, RBT, SAT, and CFT were also negative in FPA. Among 303 sera from confirmatory investigations, 269 were positive in both RBT and SAT, 21 were positive in SAT and remaining 13 were RBT-positive only. Only two sera, one positive in both tests (RBT, SAT) and one SAT-positive, were also positive in FPA. Among 32 sera originated from animals regarded as serologically positive, which reacted in RBT, SAT, and CFT, 14 gave positive results in ELISA, whereas 18 were negative. Among these ELISA-positive sera, 13 were also positive in FPA. All samples positive in SAT, RBT, and CFT, and negative in ELISA, were also negative in FPA.
13

Leptospira spp. jako potencjalny patogen czlowieka

51%
Wasinski B., Iwaniak W., Pejsak Z.
Postępy Mikrobiologii. Suplement
|
2006
|
tom 45
|
nr 1
55-60
14

Value of fluorescence polarisation assay in comparison to traditional techniques in diagnosis of porcine brucellosis

51%
Weiner M., Iwaniak W., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The aim of this study was the evaluation of fluorescence polarisation assay (FPA) in the diagnosis of porcine brucellosis in comparison with Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), 2-mercaptoethanol test, and ELISA. Eight hundred seventeen sera from pigs, including 612 sera from healthy animals, seven sera from Brucella suis bv2 culture positive animals, and 198 sera classified as false positive, originated from confirmatory investigations, were used. All sera from healthy animals, negative in RBT, SAT, CFT, and ELISA were also negative in FPA. All sera positive in serological examination, originated from Brucella infected animals, were also positive in FPA. Among sera classified as false positive almost half of the samples tested (49.49%) reacted positively in FPA. The examinations confirmed the usefulness of FPA in diagnosis of porcine brucellosis, but the method, like the other tests, does not allow resolving the problem of discrimination cross-reacting from specific antibodies.
15

Identification of Brucella DNA in lymph tissue from deer (Cervus elaphus) and wild boars (Sus scrofa) by the use of BCSP31 PCR and AMOS-PCR

51%
Weiner M., Iwaniak W., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 4
At first, a genus-specific PCR assay (BCSP31) to identify all known Brucella, generating an amplicon of 223 bp, was used. Then, positive samples were analysed with AMOS-PCR, which enables us to identify selected biovars of B. abortus, B. melitensis, B. ovis, and B. suis. The BCSP31 PCR analysis revealed that 27 (11.5%) of 235 samples originating from wild boars were positive and they all were negative in AMOS-PCR. Twenty-five were identified as B. suis biovar 2 through bacteriological examination. On the other hand, none of the 183 samples from deer was positive in PCR or bacteriological examination. The obtained results can provide a better knowledge of the potential reservoirs of infection and also should lead to great progress in molecular epidemiology.
16
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The porcine brucellosis - evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions

51%
Weiner M., Szulowski K., Iwaniak W.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 1
Forty four pigs with typical characteristics for false positive serological reactions (FPSR) were examined for the presence of Yersinia enterocolitica O:9. The positive reactions were observed in rose bengal test (RBT, N=23 sera), serum agglutination test (SAT, N=16), complement fixation test (CFT, N=9), indirect ELISA (i-ELISA, N=11) in first, and in RBT (N=14), SAT (N=8), CFT (N=7) and i-ELISA (N=18) in second examination, respectively. In bacteriological examination Y. enterocolitica was confirmed in 12 cases. Six of these isolates were identified with PCR as Y. enterocolitica O:9.
17

Isolation of Yersinia enterocolitica O:9 from cows positive in serological examination of bovine brucellosis

51%
Weiner M., Szulowski K., Iwaniak W.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The paper presents the results of bacteriological and molecular investigations on the presence of Y. enterocolitica O:9 in the head, mammary and genital lymph nodes, spleen, liver, and uterus samples originating from 58 cows slaughtered due to the positive results of serological examinations for brucellosis. All samples were cultured for Brucella and Y. enterocolitica and examined in multiplex PCR assay (mPCR), in order to identify the universal 16S rRNA Brucella sp. marker and amplify the perosamine synthetase (per) gene, specific for Y. enterocolitica O:9 only. Out of 58 examined animals, in 23 cases the presence of Y. enterocolitica was demonstrated. Typical Yersinia-suspected colonies were seen after 24-48 h incubation on Cefsulodin-Irgasan- Novobiocin (CIN) Yersinia selective solid medium. The mPCR analysis confirmed the presence of predicted amplicon of 312 bp, typical for Y. enterocolitica O:9 in 20 out of 58 lymph node samples tested and similarly, as in bacteriological examination, other samples were negative. The presence of the 16S rRNA gene of Brucella, generating the amplicon of 905 bp, was not observed in any of the samples tested.
18

Aktualne dane na temat diagnostyki zakazen owiec i koz paleczkami Brucella

51%
Iwaniak W., Szulowski K., Pilaszek J., Truszczynski M.
Medycyna Weterynaryjna
|
1997
|
tom 53
|
nr 07
377-379
19
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Characteristics of Brucella strains isolated from animals in Poland

51%
Szulowski K., Iwaniak W., Weiner M., Zlotnicka J.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 4
A total of 42 Brucella strains were isolated from animals in Poland in years 2003-2012. Most of them (N=37) originated from wild animals, 3 from cattle, 1 from pig and 1 from sheep. The strains were characterised using both bacteriological and molecular (Bruce-ladder and MLVA) methods. The examinations revealed that all strains from wild boars, hares, cattle and pigs (N=41) had the same phenotypic characteristics and were classified as B. suis biovar 2. The remaining strain, isolated from sheep, was classified as B. ovis. The molecular examination showed that all B. suis biovar 2 strains, except one, had the same molecular profile as reference strain B. suis bv2 Thomsen. Different from the others strain originated from boars imported to Poland and its VNTR profile was typical for Iberian strains.
20
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The value of Fluorescence Polarisation Assay in verification of problematic sera from pigs for brucellosis

51%
Weiner M., Szulowski K., Iwaniak W.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 4
The aim of the study was an evaluation of fluorescence polarisation assay (FPA) as a potential tool improving specificity of serological diagnosis of brucellosis in pigs. The evaluation was done by comparing the results of FPA with the results of rose bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and ELISA when false positive sera were tested. One hundred ninety porcine samples, reacting positively in at least one classical serological assay were used. We observed that among 198 sera, 104 were also positive in FPA. The studies confirmed that porcine FPA adds little as far as specificity in comparison to other methods is concerned.
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