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To assess physical activity using the Global Physical Activity Questionnaire (GPAQ) among healthy, overweight and obese Indian health professionals.The Global Physical Activity Questionnaire (GPAQ) was used to assess physical activity among 324 dental health care professionals (third-year students, final-year students, interns, and faculty). Metabolic Equivalents (MET) were used to express the intensity of physical activities, and are also used for the analysis of GPAQ data. The analysis of Variance (ANOVA) was used to compare the mean physical activity scores among dental health care professionals. The Chi Square test was used to compare categorical risk indicators and obesity (BMI). Kendall's test was used to compute the correlation between physical activity categorical indicator (CI), obesity and sedentary behaviour. The logistic regression analysis was performed to determine the importance of the factors associated with obesity. Odds ratio was calculated for all variables with 95% confidence intervals.Total physical activity measured in mean MET minutes per week was 625.6, 786.3, 296.5, and 296.5 for third-year students, final-year students, interns, and faculty respectively (p ≤ 0.05). Total energy expenditure of 0 MET minutes per week was calculated as 32.2%, 10.3%, 17.9% and 44.9% of third-year students, final-year students, interns, and faculty, respectively. Of the 211 health care professionals in high risk group 28.9% were in the third year, 19.9% in the final year, 20.4% were interns and 30.8% were faculty members. Obesity was calculated in 22.4% third-year students, 16.3% final-year students, 20.4% interns and 40.8% of faculty members. Overweight problems were seen in 19.7%, 24.7%, 24.7% and 30.8% of third-year students, final-year students, interns, and faculty members respectively (p ≤ 0.001).A significant correlation was seen between physical activity categorical indicator and BMI. A significant negative correlation was noted between physical activity categorical indicator and sedentary behaviour. A significant correlation was also noted between BMI and sedentary behavior. Physical activity is a positive health behavior with so much potential to improve public health and so few risks that it deserves to be central to any future public health strategy.
Allelopathic effects of Cyperus rotundus on banana ‘Grande Naine’ were studied in vitro as well as ex vitro. To study the allelopathic effects in vitro, the Cyperus extract was added to the multiplication medium during preparation before adjusting the pH. Of the four concentrations, 0.2 and 0.6% decreased the shoot multiplication and shoot length but 1 and 2% extract completely inhibited the shoot multiplication but induced rooting in 50 and 28% shoots, respectively. For the ex vitro studies, the Cyperus extract was added to the hydroponic medium during the hardening of in vitro raised banana plantlets. The extract of 1 and 2% concentrations decreased the shoot and root length, number of leaves and new roots, fresh weight, total chlorophyll and protein. The fluorescence intensity ratio was successively increased resulting in decreased photosynthesis.
Asynchronous pod-shatter results in significant yield losses in canola. SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) regulate pod shatter in Brassicaceae. This study describes the potential of an artificial RNA (amiRNA)-based approach for redundant silencing of SHP1/SHP2 homologs in Brassicas for introducing shatter resistance. After surveying the natural variation in Brassica SHP homologs, amiR-bnashp1 and amiR-bnashp2 were designed and processed from miR319a precursor in N. benthamiana. Analysis of thermodynamic interaction between amiRNAs and SHP1/SHP2 homologs predicted high binding energy for amiR-bnashp2 [total free energy of binding (TFEB): -23.54 kcal/mol] and not for amiR-bnashp1 (TFEB: 6.01 kcal/mol). As predicted, agroco-infiltration assay validated amiR-bnashp2-mediated cleavage of SHP1 and SHP2 homologs from B. napus, B. juncea and A. thaliana, while amiR-bnashp1 failed to direct cleavage of these homologs. In conclusion, for trait manipulation in polyploid genomes deployment of amiRNAs is suggested to silence redundant genes. Furthermore, an a priori knowledge on transcriptome potential of genome concerned is a prerequisite for predicting efficiency and specificity of target selection by amiRNA(s).
Study on the sterilizing and lethal effects of Cobalt-60 gamma radiation on the larval and adult stages of rust red flour beetle Tribolium castaneum (Herbst.) was done at 0, 10, 30, 50, 70, 100, 150, 300 and 500 gray (Gy). Irradiation severely affected pupal period, pupal formation and survivability of larvae and adults. The developmental period of the pest was found to increase along with the increase in the radiation doses. The irradiation at 70 Gy was found to cause complete sterility in the insect.
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