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Non- negative matrix factorization:a novel method to determine reference spectra of fluorescent dyesion a cellular environment

100%

Neher E.

Acta Neurobiologiae Experimentalis

2009

tom 69

nr 3

All spectrally resolved fl uorescence-based methods depend critically on the availability of accurate reference spectra. Very often spectra in a cellular environment do not agree with those measured in a cuvette. Also, for a given measurement instrument-specifi c corrections have to be applied. We recently developed a method to extract reference spetra from standard fl uorescence image stacks, such as those provided by a Zeiss Meta microscope. This method also allows one to separate spectral components of several simultaneously present dyes on a pixel by pixel basis. It should be ideal to complement lux-FRET measurements, providing reference spectra in the presence of autofl uorescence.

Technika stabilizacji potencjalu skrawka blony komorkowej

51%

Neher E., Sakmann B.

Świat Nauki

1992

nr 05

30-38

Ograniczanie wyników

1 Acta Neurobiologiae Experimentalis

1 Świat Nauki

2 Neher E.

1 Sakmann B.

1 2009

1 1992

Wyniki wyszukiwania

Non- negative matrix factorization:a novel method to determine reference spectra of fluorescent dyesion a cellular environment

Technika stabilizacji potencjalu skrawka blony komorkowej