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1

Ontogeneza i struktura neuronowa jader kompleksu suteczkowatego swinki morskiej [Cavia apera porcellus L.]

100%
Robak A.
Rozprawy i Monografie. Uniwersytet Warmińsko-Mazurski w Olsztynie
|
2004
|
tom 92
1-124
2

A cytoarchitectonic and Golgi study of the paramamillary nuclei in guinea pig

100%
Robak A.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
3

The morphological types of neurones of the medial and lateral mamillary nuclei in a newborn guinea pig: Nissl, Kluver-Barrera and Golgi studies

100%
Robak A.
Folia Morphologica
|
2002
|
tom 61
|
nr 1
21-30
The studies were carried out on the hypothalamus of 5 newborn (P0 stage) guinea pigs. The sections were impregnated according to three modifications of the Golgi technique or stained according to the Nissl and Klüver-Barrera methods. On the basis of the shape and size of perikarya, dendroarchitecture, pattern of axon as well as the inner structure of neurones, in the medial (Mm) and lateral (Ml) mamillary nuclei four morphological types of nerve cells were distinguished: cap-like with two subtypes (33% of the cell population), fusiform (35%), triangular (12%) and rounded unidendritic (21%) neurones. The majority of them possessed spines on their dendrites. The spiny cells, both cap-like and fusiform ones, were observed preponderantly, in the medial mamillary nucleus, whereas in the lateral mamillary nucleus there were mainly seen the triangular and fusiform neurones, either spiny or aspiny cells. The spineless rounded unidendritic cells were dispersed throughout the mamillary region, but they were twice as numerous in Mm as in Ml, where they were the least numerous.
4

Calcium-binding proteins immunoreactivity during ontogenesis of the limbic structures in the guinea pig

100%
Robak A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2014
|
tom 56
|
nr 1
5

Postnatal development of nuclei mamillare in guinea pig [Cavia porcellus L.]

100%
Robak A.
Folia Morphologica
|
1996
|
tom 55
|
nr 4
6

The neuronal structure of the mamillary nuclei in guinea pig: Nissl, Kluver-Barrera and Golgi studies

100%
Robak A.
Folia Morphologica
|
2000
|
tom 59
|
nr 2
The neurons of the mamillary body of adult guinea pigs were classified into four types: Type 1 — unidendritic cells with rounded perikarya (7–16 µm) and one thick primary dendrite, mostly dividing into tortuous secondary branches; Type 2 — bipolar cells: curly or simple ones with fusiform perikarya (13–22 µm); the curly-bipolar neurons possess 2 primary dendrites which may divide, even into tertiary dendrites, but each of them runs in screw-like or bending patterns; the simple-bipolar neurons have slender dendrites following a more straight route; Type 3 -multipolar cells with cap-like perikarya (10–20 µm) and 2–3 dendritic trunks originating from the base of the perikaryon and running in a wavy pattern; sometimes their dendrites possess spiny-like protrusions; Type 4 — multipolar cells with triangular or quadrangular perikarya (13–28 µm) and 3–4 dendritic trunks, poorly ramified, having a rather rectilinear course. In all types of neurons, dendritic spines are absent or rare. The majority of these neurons have a short impregnated axon originating from the perikaryon or primary dendrite.
7

The neuronal structure of the posterior part of the hypothalamus in prenatal guinea pigs

100%
Robak A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2000
|
tom 42
|
nr 1
8

Calcium-binding proteins in the hippocampal formation of the guinea pig

63%
Kolenkiewicz M., Robak A.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Calcium ions (Ca2 ) are known as a second messenger in neurons. However, the concentration of Ca2 should be maintained at an optimal level in view of their high toxicity. Calcium-binding proteins (CaBPs) bind Ca2 with high affi nity. The amount of the CaBPs is age-related and varies during brain development. The aim of this study was to analyze the distribution of CaBPs, parvalbumin (PV) and calbindin D-28k (CB), in the hippocampal formation (the dentate gyrus – DG and hippocampus proper – HP). The studies were carried out on the brains of newborn (P0) and 80-day-old (P80) guinea pigs. Labelling immunofl uorescence was performed on 10 μm-thick frozen sections. PV- and CB-immunoreactive (PV-IR, CB-IR) structures were identifi ed with antibodies against PV (1:2000, code P3088) and CB (1:2000, code C9848). Both studied parts are composed of three cellular layers. In the dentate gyrus, PV-IR cells (elongated and oval-shaped) were labelled in the hilus. In the middle (granular) layer only PV-IR dots surrounding negative cells were observed, whereas in the third (molecular) layer no immunoreactive structures were seen. In the hippocampus proper PV-IR neurons (triangular, piriform and oval-shaped) were found in each sector (CA1–CA3) of the pyramidal layer, the same as in the oriens and molecular layers. CB-IR neurons (oval-shaped, densely arranged) were observed mainly in the granular and pyramidal layers. The distribution of the studied calcium-binding proteins was similar in P0 and P80.
9

The neuronal structure of the mamillary region in postnatal stage (P20) of guinea pig

63%
Robak A., Szteyn S.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
10

The topography and cytoarchitectonics of the nuclei of supraoptic and preoptic areas in insectivores

63%
Robak A., Szteyn S.
Folia Morphologica
|
1989
|
tom 48
|
nr 1-4
11

The neuronal structure of the red nucleus in guinea pig: Klüver-Barrera and Golgi studies

63%
Robak A., Szteyn S.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
12

Calcium-binding proteins immunoreactivity in the preoptic area of the guinea pig (Cavia porcellus) during prenatal development

51%
Hermanowicz B., Bogus-Nowakowska K., Zakowski W., Robak A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2014
|
tom 56
|
nr 1
13

Distribution of cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) in the pterygopalatine ganglion of the domestic duck (Anas platyrhynchos f. domestica)

51%
Radzimirska M., Bogus-Nowakowska K., Kuder T., Robak A.
Folia Histochemica et Cytobiologica
|
2016
|
tom 54
|
nr 1
14

Changes of the chemical composition of bottom sediments in a dystrophic Lake Smolak twenty years after the end of a liming and mineral fertilisation experiment

51%
Hutorowicz J., Robak A., Hutorowicz A.
Archiwum Rybactwa Polskiego
|
1998
|
tom 06
|
nr 2
209-218
15

The neuronal structure of the dorsal lateral geniculate nucleus in the guinea pig: Golgi and Kluver-Barrera studies

51%
Szteyn S., Bogus-Nowakowska K., Robak A., Najdzion J.
Folia Morphologica
|
2001
|
tom 60
|
nr 2
79-83
On the basis of Golgi and Klüver-Barrera preparations we have distinguished four types of neurons in the dorsal lateral geniculate nucleus of the guinea pig: 1. Fusiform neurons with 1–3 thick dendritic trunks arising from each pole of the soma. The dendritic trunks branch twice dichotomically. The branches sometimes show varicosities. 2. Pear-shaped cells. From one pole of the perikaryon one or two thick dendritic trunks arise, from the opposite pole an axon emerges. The ends of the dendritic branches divide in a tuft-like manner (a characteristic feature of the interneurons). 3. Rounded neurons with 4–7 dendritic trunks without cones. The dendritic trunks branch once or twice dichotomically and give finally 2–3 thin ramifications which show a varicose course and knob-like protuberances. 4. Triangular cells with 3 thick, conically arising dendritic trunks. They bifurcate dichotomically. The surface of the dendritic trunks and of their branches is smooth.
16

The size of neurons in the nuclei of the mammalian amygdala:the comparative morphometric study

51%
Rowniak M., Robak A., Szteyn S.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
17

The neuronal structure of the striatum in the common shrew (Sorex araneus):Golgi and Nissl studies

51%
Wasilewska B., Najdzion J., Robak A., Sztejn S.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
18

The neuronal structure of the ventromedial and infundibular nuclei in the guinea pig: Nissl and Golgi study

51%
Bogus-Nowakowska K., Szteyn S., Robak A.
Folia Morphologica
|
2001
|
tom 60
|
nr 4
309-315
The studies were carried out on the mesencephalons of adult guinea pigs. On the basis of the Golgi technique, as well as the Nissl and Klüver-Barrera methods, four types of neurons were distinguished in the ventromedial nucleus (VMH) and infundibular nucleus (Ni): 1. Rounded neurons (perikarya 12–18 µm) with 3–4 dendritic trunks, which divide once, twice or not at all. The dendritic branches possess varicosities and knob-like spines. These neurons predominate in VMH. 2. Fusiform neurons (perikarya 15–28 µm) with 2 dendritic trunks, which arise from the opposite poles of the cell body. Bead-like protuberances and knob-like processes are observed on the dendrites. These neurons are the most numerous in Ni. 3. Triangular neurons (perikarya 15–22 µm) possess three thick, conical dendrites, which bifurcate dichotomically. Bead-like appendages and knoblike processes were seen on the dendritic surface. 4. Multipolar neurons (perikarya 18–22 µm) with 4–5 dendritic trunks, which are poorly ramified. The dendritic branches are smooth, but varicosities can be observed on their surface. In all types of neurons an axon was observed to arise either from the dendritic trunk or from the soma.
19

Expression of calretinin and calbindin in the cingulate cortex of the guinea pig

51%
Hermanowicz-Sobieraj B., Bogus-Nowakowska K., Rowniak M., Robak A.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: The cingulate cortex (CC), a part of the limbic cortex, is one of the major components of the Papez circuit. Mammalian cerebral cortex contains excitatory pyramidal neurons (70–80%), which use glutamate as neurotransmiter, and interneurons (20–30%), mostly inhibitory, using GABA as principal neurotransmiter. The maintenance a balance between these neurotransmitters is essential for proper functioning of neurons. GABAergic neurons deficit is often related to neurodegenerative disorders. Markers for GABAergic neurons are calcium-binding proteins: calretinin (CR) and calbindin (CB), which may act as calcium sensors as well as both fast and slow calcium buffers. AIM(S): The aim of the study was to describe the distribution of CR and CB and compare expression of both CaBPs at transcriptional and final product levels in the cingulate cortex of the adult guinea pig. METHOD(S): Genes expression of CR and CB was measured on mRNA by quantitative real-time PCR (qPCR) analysis. Total RNA was isolated using Total RNA Mini and then was reverse transcribed to cDNA using Maxima cDNA Synthesis Kit. qPCR was conducted using SYBR® Green JumpStartTMTaqReadyMixTM. To visualize CR and CB immunoreactivity frozen sections were undergone for routine single immunofluorescence labelling, using solution of antibodies raised against CR and CB. RESULTS: The immunohistochemical study indicates the presence of CR and CB in the whole CC. The number of CB-positive perikarya was lower than the CR ones. CR-positive perikarya in comparison to CB-positive, were more numerous in the superficial than in the deep layers of the CC. The qPCR analysis showed that the mRNA expession for CR was higher than for CB. CONCLUSIONS: The CB and CR mRNA expression level revealed by qPCR correlate with their protein abundance level revealed by immunohistochemistry. Calretinin expression was higher than calbindin at both levels. FINANCIAL SUPPORT: Co‑financing the scientific studies for young scientists or PhD students at the Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn (ID:12.620.026-300).
20

Developmental changes of calbindin immunoreactivity in the preoptic area of the male guinea pig (Cavia porcellus)

51%
Bogus-Nowakowska K., Hermanowicz-Sobieraj B., Rowniak M., Robak A.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Calbindin (CB) is one of the members of the EF-hand family of calcium-binding proteins which are involved in controlling intracellular calcium ion homeostasis. It may act as Ca2+ “fast” buffers and also as Ca2+ sensors. Intracellular calcium ions play an important role in immature and mature neurons. During early stages of development, calcium ions are involved e.g. in neuronal differentiation and plasticity, migration of neurons, or extension of neuronal processes. The preoptic area (POA) is a key structure which takes part in many autonomic functions (for example thermoregulation, thirst or hunger) as well as in reproduction and maternal behaviour, especially for pup retrieval as well as the onset of parental behaviour in females and males. AIM(S): The aim of the study was to examine the distribution of CB expression during the development of the preoptic area in the guinea pig by means of immunohistochemistry. METHOD(S): Brains from fetal stages (E50, E60), newborns (P0) and postnatal stages (P10, P20, P40, P100) were fixed in 4% paraformaldehyde in phosphate buffer and then cryoprotected. Frozen sections were processed for two immunohistochemical methods: an immunoenzymatic and immunofluorescence. RESULTS: Calbindin was highly expressed in the preoptic area of the male guinea pig, especially in the periventricular region. CB- immunoreactive (-ir) perikarya, fibers and punctate structures were observed at each examined stages. CB-ir perikarya were the most numerous at E50 and the least numerous at P100. The CB-ir neurons had oval, rounded or polygonal perikarya and some of them had processes of various length which emerged from perikarya. CB-ir fibers differed according to lengths. CONCLUSIONS: The highest expression of CB in the preoptic area at E50 coincides well with major developmental events (i.e. eyes opening) which in the guinea pig occur just before E50 stage.
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