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1

Zróżnicowanie genotypowe i analiza stabilności plonowania i parametrów technologicznych nasion fasoli karłowej (Phaseolus vulgaris L.)

100%
Boros L., Wawer A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 555
Oceniono efekty genotypowe oraz analizowano stabilność plonowania i parametry technologiczne nasion krajowych odmian fasoli karłowej uprawianej przez sześć sezonów wegetacyjnych w Radzikowie. Stwierdzono istotność różnic genotypowych dla sezonów wegetacyjnych oraz istotność interakcji genotypowo-sezonowych dla wszystkich badanych cech. Analiza komponentów wariancji wykazała, że dla masy 100 nasion oraz zawartości łuski, zawartości popiołu i czasu gotowania - komponent genotypowy znacząco przewyższał komponent genotypowo-sezonowy. Absorpcja wody, elektroprzewodnictwo roztworów to parametry o wysokim udziale obydwu komponentów wariancji. Najwyższy udział komponentów wariancji, innych niż genotypowy, stwierdzono dla plonu nasion z poletka oraz zawartości białka. Analiza stabilności według procedury Francis & Kannenberg pozwoliła na pogrupowanie i wyodrębnienie genotypów łączących pożądany potencjał plonowania z korzystnymi, przynajmniej dwiema, cechami kulinarnej jakości oraz niską zmiennością w latach. Wytypowane odmiany mogą być wykorzystane w pracach hodowlanych w celu poprawy określonych parametrów w procesie hodowlanym.
2

Zróżnicowanie odmianowe parametrów fizyko-chemicznych i czasu gotowania nasion grochu siewnego (Pisum sativum L.)

100%
Boros L., Wawer A.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2011
|
nr 260-261
3

Garden pea varietal susceptibility to Mycosphaerella pinodes and its effect on yield components of single plants

100%
Boros L., Wawer A.
Vegetable Crops Research Bulletin
|
2009
|
tom 70
4

Ocena podatności odmian grochu siewnego na porażenie grzybem Mycosphaerella pinodes w testach w warunkach kontrolowanych i polowych z kontrolowaną infekcją

100%
Boros L., Wawer A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2007
|
tom 522
W pracy dokonano oceny genotypowego zróżnicowania poziomu odporności odmian i linii kolekcyjnych grochu siewnego na porażenie grzybem Mycosphaerella pinodes oraz wskazano potencjalne źrodła podwyższonej odporności dla praktycznej hodowli. Badaniami objęto 28 genotypów grochu (16 odmian z Krajowego Rejestru, 4 linii IHAR, 3 odmiany zagraniczne i 5 linii z Pullman o znanej odporności) w celu oceny zakresu zmienności nasilenia zgorzeli siewek oraz podatności/odporności liści i łodyg siewek w warunkach kontrolowanych na porażenie grzybem M. pinodes. Formy te poddano również ocenie w doświadczeniu polowym z kontrolowaną infekcją. Stwierdzono istotność genotypowego zróżnicowania podatności na porażenie M. pinodes w warunkach kontrolowanych i polowych z kontrolowaną infekcją. Wyodrębniono grupę genotypów grochu o niższej podatności na porażenie M. pinodes. Przeanalizowano zależności występujące pomiędzy wynikami ocen w różnych testach. Uwzględnienie wyników oceny podatności będzie pomocne hodowcom w wyborze komponentów do krzyżowań.
5

Poziom i stabilność plonowania odmian soi o różnej wczesności w zróżnicowanych warunkach agro-klimatycznych

81%
Boros L., Wawer A., Borucka K.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2019
|
nr 285
6

Wplyw zageszczenia roslin w lanie na cechy uzytkowe trzech wasolistnych genotypow grochu siewnego [Pisum sativum L.]

81%
Sawicki J., Boros L., Wawer A.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2000
|
nr 214
253-261
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Morphological, phenological and agronomical characterisation of variability among common bean (Phaseolus vulgaris L.) local populations from The National Centre for Plant Genetic Resources: Polish Genebank

81%
Boros L., Wawer A., Borucka K.
Journal of Horticultural Research
|
2014
|
tom 22
|
nr 2
8

Warta - nowa odmiana fasoli karlowej; wartosc rolnicza oraz parametry technologiczne nasion

81%
Boros L., Szyrmer J., Wawer A.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
2001
|
tom 76.1
25-33
9

Response of pea (Pisum sativum L.) cultivars and lines to seed infection by Ascochyta blight fungi

81%
Marcinkowska J., Boros L., Wawer A.
Plant Breeding and Seed Science
|
2009
|
tom 59
10

Agronomic performance and technological value of dry bean genotypes [Ph.vulgaris L.]

80%
Boros L., Waszkiewicz-Robak B., Szyrmer J., Wawer A.
Vegetable Crops Research Bulletin
|
2001
|
tom 54
|
nr 1
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cerebral administration of alpha-synuclein monomers modulates inflammatory reaction in nigro-striatal system

71%
Szneider-Pacholek A., Joniec-Maciejak I., Wawer A., Ciesielska A., Mirowska-Guzel D.
Journal of Pre-Clinical and Clinical Research
|
2019
|
tom 13
|
nr 1
12

Neuroprotective properties of cystamine in MPTP-induced murine model of Parkinson’s disease

71%
Wawer A., Joniec-Maciejak I., Sznejder-Pacholek A., Wojnar E., Mirowska-Guzel D.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: Parkinson’s disease (PD) is a common neurodegenerative movement disorder. It is characterized by a progressive degeneration of dopaminergic substantia nigra neurons projecting to the striatum, as well as, by an accumulation of intraneuronal Lewy bodies containing misfolded a‑synuclein. Neurodegeneration is coincident with a decrease in dopamine, the dopamine transporter, and the dopamine metabolites levels in PD brain. Current therapeutic approaches for the treatment of PD are only symptomatic and do not block neuronal loss. Recently, an enormous amount of work has been conducted to identify molecules that could be used as neuroprotective agents. One of them is cystamine – the inhibitor of transglutaminases activity. Transglutaminases are involved in the formation of a-synuclein aggregates, therefore blocking of its activity may prevent the progression of PD. AIM(S): The aim of the present study was to examine the effects of cystamine on neurodegenerative processes in the murine model of PD. METHOD(S): Male 1‑year‑old C57Bl/10 Tar mice were used in this study. Cystamine was injected intraperitoneally for 14 days, beginning 13 days prior to 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine (MPTP) intoxication. Changes in the mRNA level of inflammatory factors in striata were examined using Real‑Time PCR. Neurotransmitter levels in striata were evaluated by high‑performance liquid chromatography (HPLC). RESULTS: Our study demonstrated that chronic administration of cystamine before MPTP intoxication improved striatal levels of dopamine and its metabolites, as compared to MPTP‑treated groups. We also observed an inhibition of inflammatory reaction induced by MPTP (lower expression of microglia marker and proinflammatory interleukin). CONCLUSIONS: Cystamine exerts anti-inflammatory effects and preserves nigrostriatal function after MPTP intoxication in PD. However, further research must be conducted to provide more evidence of a protective role of cystamine in PD.
13

Cerebral administration of alpha synuclein modulates inflammatory reaction in the nigro striatal system: A comparison of males and females

71%
Sznejder-Pacholek A., Joniec-Maciejak I., Wawer A., Wojnar E., Mirowska-Guzel D.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: The risk of developing Parkinson’s disease (PD) is twice as high among men as among women. Estrogens appear to be a protective factor. The progression of PD is characterized by inflammation, especially the activation of the microglia. The data suggests that increased levels of α‑synuclein (ASN) can induce microglia activation. Activated microglial cells release pro‑ and anti‑inflammatory cytokines. AIM(S): The aim of this study was to investigate the role of increased ASN monomers concentration as a major pathogenic factor causing microglia response and changes in the expression of mRNA of inflammatory cytokines (i.e., interleukin 1α (IL‑ α), IL‑10, IL‑12 and tumor necrosis factor α (TNFα)) in the striatum (ST). We also examined the levels of ionized calcium binding adaptor molecule 1 (Iba‑1). We evaluated the differences between the genders after injection of ASN. METHOD(S): Male and female C57Bl/10 Tar 9-month- ‑old mice were used in this study. Human recombinant ASN was bilaterally administered into ST (single treatment – 4 μg/structure, 8 μg per brain) and mice were decapitated after 4‑or 12‑weeks post injection. Changes in the level of inflammatory factors in ST were evaluated using Real‑Time PCR. RESULTS: We observed increased levels of a microglia marker, Iba1 protein, in all experimental groups after 4 weeks of injection of ASN into the ST, with the highest differences in the FOVA group (female after ovariectomy). IL‑10 mRNA levels were significantly elevated in the FOVA group at 4 weeks after ASN administration intothe ST as compared to the ovariectomy control group. We noticed differences in levels of IL‑1α, IL‑12, and TNFα mRNA between the genders after injection of ASN. CONCLUSIONS: Our results support the hypothesis of pro‑inflammatory actions of ASN monomers. Injection of ASN into the ST induces microglia activation. Estrogens appear to be an important factor in the inflammatory reaction in the murine model of Parkinson’s disease after cerebral administration of ASN.
14

The effect of alpha-synuclein on initiation of inflammatory reaction in the murine brain

71%
Joniec-Maciejak I., Wawer A., Sznejder-Pachołek A., Wojnar E., Mirowska-Guzel D.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Parkinson’s disease (PD), one of the most common neurological disorder, is characterized by the loss of dopaminergic neurons in substantia nigra and striatum (ST). The typical reaction of central nervous system (CNS) on neurodegenerative processes is microglia activation and the inflammatory reaction. The data suggests that increased level of α‑synuclein (ASN), a small protein which is the major component of Lewy bodies, can induce microglia activation. Activated microglial cells release proinflammatory and potentially cytotoxic substances like cytokines. Till now, little is known about in vivo effects of exogenous ASN monomers on initiation of neuroinflammation and neurodegeneration. AIM(S): The aim of the present study was to examine the effect of increased ASN monomers concentration on microglia response and expression of pro‑ and anti‑inflammatory cytokines (interleukin 1α (IL‑1α), IL‑10, IL‑12) in the ST. METHOD(S): Male and female C57Bl/10 Tar mice 9 month-old were used in this study. Human recombinant ASN was bilaterally administered into ST (single treatment – 4 μg / structure, 8 μg per brain) and mice were decapitated after 4 or 12 weeks post injection. The changes in the level of inflammatory factors in ST were evaluated using Real-Time PCR and enzyme-linked immunosorbent assay (ELISA). RESULTS: We observed increased level of a microglia marker – ionized calcium-binding adapter molecule 1 (IBA1) protein after ASN injection into ST. We noticed also some differences in the level of one of the most important pro inflammatory cytokines – IL‑1α. CONCLUSIONS: Our study showed that monomers of ASN are strongly involved in the inflammatory reaction in the murine CNS. Further studies are required to reveal the detailed mechanism of the influence of ASN on neuroinflammation in course of Parkinson’s disease. FINANCIAL SUPPORT: This study was supported by Grant No 1M9/PM 2/16 (Medical University of Warsaw). Research subject was implemented with CePT infrastructure financed by the European Union – The Europan Regional Development Fund within the operational programme “Innovative economy for 2007–2013.
15

The influence of alpha-synuclein on neurodegenerative processes in the murine brain

71%
Wojnar E., Wawer A., Joniec-Maciejak I., Sznejder-Pachołek A., Mirowska-Guzel D.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Parkinson’s disease (PD) is one of the most common neurodegenerative disorders. It is characterized by a progressive loss of dopaminergic neurons accompanied by a decreased concentration of dopamine (DA) and its metabolites in the striatum (ST). Experimental and clinical data indicate that α‑synuclein (ASN) plays an important role in many processes observed in the brains of patients with PD, such as disorder homeostasis of dopamine (DA) or initiate of oxidative stress. Changes in ASN levels due to its aggregation, overexpression or decreased expression may disrupt DA homeostasis and contribute to the neurodegeneration process observed in PD. AIM(S): The aim of the present study was to investigate the influence of cerebral injection of ASN on neurotransmitters level in ST. We also examine the expression of tyrosine hydroxylase gene (TH, the rate-limiting enzyme of catecholamine biosynthesis) and tissue transglutaminase 2 gene (TG2; an enzyme involved in aggregation of ASN). METHOD(S): Male and female C57Bl/10 Tar mice 9 month-old were used in this study. Human recombinant ASN was bilaterally administered into ST (4 μg/structure, 8 μg per brain) and mice were decapitated after 4 or 12 weeks post injection. Concentration of striatal neurotransmitters were measured by high performance liquid chromatography (HPLC). The gene expressions were examined by Real Time PCR. RESULTS: Intracerebral administration of ASN monomers led to changes in concentrations of striatal neurotransmitters but do not affect the expression of TH gene. The ASN administered intracerebrally into ST increases striatal expression of TG2 gene, which can lead to enhanced ASN aggregation. CONCLUSIONS: The biochemical changes observed after ASN administration may initiate further neurodegenerative processes and probably represent a very early stage in development of PD. Further research must be conducted to better understand the crucial role of ASN in the neurodegenerative process in PD. FINANCIAL SUPPORT: This study was supported by Grant No. 1M9/PM 2/16 (Medical University of Warsaw). Research subject was implemented with CePT infrastructure financed by the European Union – The Europan Regional Development Fund within the operational programme “Innovative economy for 2007–2013.
16

Jak to się robi na Wyspach

60%
Wawer A.
Koń Polski
|
2009
|
tom 44
|
nr 07
17

The influence of IL-10 on the inflammatory reaction changes in the mice after 1-methyl-4-phenyl-1,2,3,6-tertahydropyridine (MPTP) treatment

51%
Sznejder A., Joniec-Maciejak I., Ciesielska A., Schwenkgrub J., Wawer A., Bankiewicz K., Czlonkowska A., Czlonkowski A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Parkinson’s disease (PD) is one of most frequent neurological disorder characterized by the loss of dopaminergic neurons in substantia nigra and striatum. The typical reaction of central neural system (CNS) on neurodegenerative processes is microglia activation and the inflammatory reaction. Microglia activation stimulates astrocytes response, playing important role in neuroimmune reaction. Microglia cells secrete two types of mediators of the inflammatory process: anti- and pro- inflammatory. In the first stage of Parkinson’s disease, pro-inflammatory cytokines have important meaning. We investigated the effect of an adenoassociated viral vector (AAV2) containing the complementary DNA (cDNA) for human interleukine 10 (hIL-10). The aim of the present study was to examine the evaluation of inflammatory reaction changes following increased concentration of hIL-10 in the murine model of PD induced by MPTP. Male C57BL mice 12 month-old were used in this study. AAV2 vector was bilateraly administered into striatum at 7, 21, 28 days prior to MPTP intoxication. We observed changes in the morphology of microglia cells, infiltration of lymphocytes T (population of CD3+, CD4+ and CD8+) and some differences in the level of one of the most important pro inflammatory cytokines – IL-1α. Our study showed that IL-10 is strongly involved in the inflammatory reaction in the murine model of Parkinson’s disease induced by MPTP. After MPTP intoxication we observed the increase of activated microglia cells, infiltration of lymphocytes T and higher level of IL-1α mRNA. AAV2-hIL-10-treated mice displayed a significant decrease in the activated microglia cells, elevated expression of IL-10 receptors observed on glia cells, strong infiltration of lymphocytes T (mainly CD4+ and CD3+, less CD8+) and minor expression of IL-1α mRNA. Further research must be conducted to provide more evidence of protective role of IL-10 in Parkinson disease.
18

The effect of human interleukin-10 on the nitric oxide synthases expression in MPTP- based model of Parkinson's disease

51%
Schwenkgrub J., Joniec-Maciejak I., Ciesielska A., Sznejder A., Wawer A., Bankiewicz K., Czlonkowska A., Czlonkowski A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Parkinson’s disease (PD) is a progressive degenerative disorder, which etiology and pathogenesis remains unknown. Post mortem analysis of PD brain and studies on neurotoxic animal models of PD have provided evidence to support the involvement of oxidative stress and neuroinflammatory processes in the pathogenesis of PD. The high level of nitric oxide (NO) is produced by iNOS during the neuroinflammatory process caused by MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) treatment. Under pathological condition NO can easily react with superoxide to form peroxynitrite (ONOOˉ), which is a strong oxidant. In the present study was examined the influence of the increased concentration of IL-10 (an anti-inflammatory cytokine) on the NOS expression in mouse model of PD induced by MPTP. One year-old male C57Bl mice were used in this study. An adeno-associated viral vector expressing the gene for human interleukin-10 (hIL-10) was used to transduce striatal cells 4 weeks prior to MPTP intoxication. Mice were sacrificed at the different time intervals: 1, 7 and 21 days after MPTP injection. Immunohistochemical and western blot analyses provide evidence for the protective properties of AAV2-hIL-10 in the MPTP-induced model of PD. There were reduction in the dopaminergic neuron quantity in SNpc and tyrosine hydroxylase protein in the striatum after MPTP injections, whereas in the group additionally treated with AAV2-hIL10 neuroprotection was observed. Treatment with AAV2-hIL-10 suppressed the MPTP-induced increase in iNOS and 3-nitrotyrosine (3-NT) expression in the midbrain.
19

The influence of AAV-mediated gene transfer of human interleukin 10 on the neurodegenerative process in the murine model of parkinson's disease

51%
Wawer A., Joniec-Maciejak I., Schwenkgrub J., Sznejder A., Ciesielska A., Bankiewicz K., Czlonkowska A., Czlonkowski A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Parkinson’s disease (PD) is one of the most common neurodegenerative disorders. It is characterized by a progressive loss of dopaminergic neurons, which occurs mainly in the substantia nigra (SN), resulting in the loss of nerve terminals, accompanied by a decreased concentration of dopamine (DA) and its metabolites in the striatum. Concurrently with neurodegeneration, a chronic inflammation occurs in the affected regions of the brain. Mechanisms and etiology of the neurodegeneration are still unknown. One potential strategy for therapy of PD is to reduce the neurodegeneration by inhibiting the inflammatory reaction. Nowadays, there are high hopes for the gene therapy based treatment. This involves using a noninfectious virus administered directly into the brain. In this study a transfer of AAV vector containing the complementary DNA for human interleukin 10 (IL-10) into the nigrostriatal pathway was used. IL-10 is one of the major antiinflammatory cytokines. The aim of the present study was to examine the expression of human IL-10, administered into striatum by viral vector AAV2-hIL-10 and investigate the influence of this cytokine on neurodegenerative process in the murine model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). One year old male C57Bl mice were used in this study. The expression of human IL-10 was measured by enzyme-linked immunosorbent assay (ELISA). To evaluate the influence of the human IL-10 on neurodegeneration concentrations of striatal DA, 3,4-dihydroxyphenyl acetic acid and homovanillic acid were measured by high performance liquid chromatography (HPLC). The findings demonstrate human IL-10 secretion in the mouse brain after striatal infusion of AAV2-hIL-10. This study suggests that human IL-10 delivered by an AAV2 vector preserves nigrostriatal function after MPTP intoxication (lower decrease in DA concentration). IL-10 can play a vital role in inhibition of inflammatory reaction or surviving cells stimulation.
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