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The objective of the study was the phenotypic characteristics of thymocytes and T-lymphocytes and the evaluation of T cell function in the course of experimental trichinellosis in mice. It was found that the cortical part of the thymus was significantly diminished and the expression of Thy, Ly1 and Ly2 antigens were decreased during the intestinal phase of the parasitic invasion. Simultaneously, the increase of Thy⁺ bearing cells was observed in peripheral lymphatic tissue (spleen and lymph nodes) during this and the next early muscular phase of the invasion of T. spiralis. The expression of L3T4 and Ly2 antigens was also higher than or at least the same as in the control animals. The results of the evaluation of T lymphocytes reactivity show that the parasitic invasion results in changes in cellular immunity as was demonstrated by GvH reactions. These changes are dependent on both the intensity of the parasitic invasion and the phase of infection. The possible mechanisms of the modulation (stimulation or inhibition) of host immunity were discussed.
Activity of spleen lymphocytes derived from T. spiralis-infected C3H/w mice in the local GvH reaction was assessed using the popliteal lymph node GvH assay. It was found that splenocytes obtained on day 10 of the infection were substantially more active in the reaction than the spleen cells collected from uninfected donors. This effect correlated inversely with the infectious dose of the parasite, i.e. cells obtained from mice infected with 500 larvae per mouse were less efficient stimulators of the GvH reaction than splenocytes isolated from mice infected with 200 larvae per mouse. On day 30 of the infection activity of spleen T cells in the GvH reaction was suppressed in comparison to the control splenocytes but on day 60 post infection this activity returned to the baseline level. The above variations in the activity of splenic T lymphocytes in the local GvH reaction were readily quantitated by comparison of the three appropriate parameters assessed in the popliteal lymph nodes of both the infected and control animals and expressed as the mass, cellularity, and proliferation coefficients, respectively.
The activity of splenocytes obtained from C3H/w mice during various phases of infection with T. spiralis was assessed using the GvH reaction in irradiated B6C3F₁ recipients of spleen cells. It was found that splenocytes obtained from mice during the intestinal phase of infection (i.e. on day 10 of infection) stimulated the GvH reaction whereas splenocytes obtained during later periods of infection (i.e. on days 20 or 30 of infection) inhibited the GvH reaction. The inhibitory effect persisted until day 60 of infection. Decreased function of T cells in the GvH reaction, as observed during the early muscle phase of trichinellosis (i.e. 30 days post injection of the larvae) was less pronounced in hosts infected with T. spiralis than in those infected with T. pseudospiralis. In addition, significant impairment of the GvH reaction was observed following the mixed transplant of splenocytes obtained from both uninfected C3H/w mice as well as from B6C3F₁ mice infected with T. pseudospiralis. The results indicate that the activity of T lymphocytes in mice infected with T. spiralis changes significantly during different phases of infection.
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Wplyw metali ciezkich na uklad pasozyt-zywiciel

63%
Now in the time especially intensive development of industry the question of environment pollution became very important. According to review of the problem heavy metals are one of the most hazardous constituents of xenobiotics. Their toxic effects, among other, are manifested directly on the elements of the immune system as well on the modification of the immune response. The influence of heavy metals on the host-parasite system is rather poorly recognized and here we are discused about it, according to literature and own experience. Special attention is paying to their impact on the immune response of infected and treated with lead, cadmium or mercury host and on the parasite. The definitive host eg. fishes and their some parasite can be used as potential accumulation indicators of aquatic environment of heavy metal pollution.
Lymphocyte-induced angiogenesis (LIA), a vascular response was observed when allogenic immunocompetent lymphocytes of C3H/w mice infected with Trichinella spiralis were inoculated intradermally into irradiated BALB/c. It was shown that intestinal phase of T. spiralis infection stimulated mainly CD5 T cells dependent angiogenesis. The enhancement of angiogenesis did not occur in the early and late muscular phases of the infection. It is suggested, that changes in the intensity of angiogenesis may play a role in spreading of new-born larvae in the host.
The aim of this study was to estimate the development of P. aeruginosa infection in mice infected with T. spiralis or T. pseudospiralis. The investigations were carried out on the following strains of mice: C57B1, C3H and B6C3F1-. 20 days post parasitic infection P. aeruginosa were administrated by inhalation or by intraperitoneal injection. After 4 hrs development of infection in mice was evaluated (the number of bacteria in lung or in the liver tissue were counted by plating). It was found that the bacterial infection developed with the highest rate in C3H mice followed by C57B1/6, in comparison with control mice. In C3H mice infected with T. pseudospiralis, the number of bacteria was higher in lung and liver tissue, in comparison with those infected with T. spiralis. In B6C3F1 hybrid the infection rate was significantly lower after intraperitoneal administration in T. spiralis infected mice. In our experiments, the development of P. aeruginosa infection in mice with trichinellosis was dependent on the strain of mice and the routes of bacteria administration.
W pracy przeprowadzono ocenę aktywności ATP-azowej i fosfatazowej błony śluzowej jelita cienkiego myszy, które zatruwano dożołądkowo azotynem sodowym przed oraz w trakcie rozwoju włośnicy.
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