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1

Zroznicowanie efektywnosci androgenezy u wybranych genotypow papryki rocznej [Capsicum annuum L.]

100%
Nowaczyk P., Kisiala A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2007
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tom 517
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nr 2
861-866
The study dealt with the diversity of androgenesis potential among selected pepper (Capsicum annuum L.) genotypes, which are commonly cultivated in Poland. Total of 4430 anthers of 8 morphologically and physiologically different breeding lines, varieties, and hybrid pepper genotypes were cultured according to Dumas de Vaulx et al. (1981), with slight modifications concerning culture media composition and the length of anthers incubation period on CP medium. In the course of experiment the effectiveness of androgenic embryos and mature plants production was analysed. As a result of this study total number of 127 androgenic embryos and 108 mature plants were obtained. Additionally, a large diversity among all the analysed pepper genotypes was observed. Considerably more responsive ones turned out to be the hybrid genotypes, as compared with their parental forms. The best results were obtained for (ATZ1xCDT)F₁ hybrid (9.8%), while the lowest androgenesis potential presented TG breeding line (0.5%). As the large diversity in androgenesis effectiveness among local pepper genotypes was confirmed, it is worth to concern the necessity of determination of this characteristic for the pepper germplasm stored in pepper gene banks.
2

Analiza morfologiczna i cytometryczna kalusa otrzymanego z kultur pylników wybranych genotypów papryki rocznej (Capsicum annuum L.)

100%
Nowaczyk P., Kisiala A.
Prace Wydziału Nauk Przyrodniczych. Seria B. Prace Komisji Nauk Rolniczych i Biologicznych
|
2004
|
tom 39[52]
3

Effect of selected factors on the effectiveness of Capsicum annuum L. anther culture

100%
Nowaczyk P., Kisiala A.
Journal of Applied Genetics
|
2006
|
tom 47
|
nr 2
The primary aim of the study was to establish the effectiveness of induced androgenesis in in vitro anther culture of two pepper (Capsicum annuum L.) breeding lines - ATZ1 and PO, and a hybrid between these two lines (ATZ1 X PO)F₁. Anther culture was maintained according to the method developed by Dumas de Vaulx et al. (1981) with some modifications. The experiment revealed that the effectiveness of androgenesis ranged from 4% for the ATZ1 line to 1.5% for the (ATZ1 x PO)F₁ and strongly depended on the developmental stage of flower buds, as well as the conditions for anther culture maintenance. The development of androgenic embryos was successfully induced only in anthers which originated from the flower buds with petals equal or slightly longer than sepals and there was a clear relationship between the length of the period of anther induction on CP medium and the level of kinetin in R₁ regeneration medium.
4

Analiza izoenzymatyczna roślin otrzymanych w kulturach pylników mieszańcowych genotypów papryki (Capsicum spp.)

81%
Olszewska D., Kisiala A., Nowaczyk P.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2009
|
tom 539
|
nr 2
Analiza białek izoenzymatycznych jest metodą, która na podstawie stopnia wyrównania genetycznego roślin pozwala na potwierdzenie mikrosporowego pochodzenia androgenicznych regenerantów. W przeprowadzonym eksperymencie oceniono cztery gatunki papryki C. annuum L., C. frutescens L., C. chinense JACQ. i C. baccatum L. var. pendulum oraz rośliny należące do siedmiu populacji androgenicznych, otrzymanych w kulturach pylników dwóch międzygatunkowych mieszańców pokolenia F1: C. frutescens x C. chinense oraz C. annuum x C. baccatum. Dla wszystkich badanych roślin porównano wzory prążkowe następujących izoenzymów: izomerazy glukozofosforanowej, mutazy glukozofosforanowej, dehydrogenazy izocytrynianowej oraz dehydrogenazy jabłczanowej. Izoenzymy PGM oraz IDH różnicowały cztery oceniane gatunki z rodzaju Capsicum. Na podstawie analizy wzorów prążkowych izoenzymu IDH stwierdzono również zróżnicowanie genetyczne pomiędzy androgenicznymi populacjami mieszańca C. frutescens x C. chinense. Jednocześnie, wyniki przeprowadzonych analiz wykazały wyrównanie genetyczne wszystkich badanych populacji pod względem analizowanych izoenzymów.
5

Study of individual plant responsiveness in anther cultures of selected pepper (Capsicum spp.) genotypes

81%
Niklas-Nowak A., Olszewska D., Kisiala A., Nowaczyk P.
Folia Horticulturae
|
2012
|
tom 24
|
nr 2
6
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Content available

Biometrical characteristics of R2 generation of anther-derived pepper (Capsicum spp.) plants

81%
Kisiala A., Olszewska D., Niklas-Nowak A., Nowaczyk P.
Acta Agrobotanica
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2011
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tom 64
|
nr 3
Eight anther-derived DH lines of pepper hybrids: two red-fruited (AP14, AP15) and two yellow lines (AP25 and AP32) of C. annuum (ATZ1 × PO)F2, two lines of (C. frutescens × C. annuum)F1 (FA1, FA2), and two of (C. frutescens × C. chinense)F1 (FCH2, FCH3), were studied regarding important morphological plant and fruit characters. C. annuum breeding line ‘ATZ1’ was used as a standard. The following traits were evaluated: total fruit yield, fruit weight, weight of placenta with seeds, technological matter, pericarp thickness, extract content, dry matter content, weight and number of seeds per fruit. The level of homogeneity within the DH lines was analysed with a one-way analysis of variance, additionally the values of coefficient of variation (CV) were determined for the tested plant characters. The highest phenotypic uniformity was noted for the red-fruited lines obtained from anthers of (ATZ1 × PO)F2 and for the DH lines of (C. frutescens × C. annuum) F1, while the least uniform were FCH2 and FCH3 lines of (C. frutescens × C. chinense)F1. The DH lines AP14 and AP15, as well as FA1 and FA2 were phenotype homogeneous in respect of weight of fruit, technological matter, pericarp thickness and extract content. For these parameters, also the values of CV were the lowest. Statistically significant interline polymorphism between the androgenic lines of the same origin was detected among the lines of C. annuum (ATZ1 × PO)F2.
7

In vitro anther culture of Capsicum frutescens L. red- and yellow-fruited forms

81%
Nowaczyk P., Kisiala A., Olszewska D.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2005
|
tom 47
|
nr 1
8

The assessment of doubled haploid lines obtained in pepper (Capsicum annuum L.) anther culture

81%
Olszewska D., Kisiala A., Nowaczyk P.
Folia Horticulturae
|
2011
|
tom 23
|
nr 2
9

In vitro androgenic response of some pepper (Capsicum) genotypes to different levels of kinetin

81%
Kisiala A., Niklas-Nowak A., Orlinska M., Nowaczyk P.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 1
10

Induced androgenesis of Capsicum frutescens L.

80%
Nowaczyk P., Kisiala A., Olszewska D.
Acta Physiologiae Plantarum
|
2006
|
tom 28
|
nr 1
The aim of the research was to make a preliminary determination of the effectiveness of the in duc tion of haploids in Capsicum frutescens L. In order to induce androgenesis red and yellow fruit forms of species were used, each bred by the re searchers on their own. The experiment was per formed in October. An ther cultures were conducted according to a modified me - thod developed by Dumas et al. (1981) for C. annuum L. The anthers were laid on CP medium con taining 0.01 mg·dm⁻³ 2.4-D and 0.01 mg·dm⁻³ kinetin, with the addition of 0.5 g·dm⁻³ of activated carbon and 5 mg×dm⁻³ of silvernitrate, solidified with 8 g·dm⁻³ of agar. The cul tures were in cu bated in the dark at 35 deg C for 8 days. Next they were trans ferred to 25 deg C under a 12-hour photoperiod. After 14 days of induction, anthers were trans ferred to R₁ medium supplemented with 0.1 mg·dm⁻³ kinetin. Obtained embryos were subsequently transplanted onto V₃ hormone-free me dium and well growing plants were planted in green houses. The efficiency of androgenesis for both C. frutescens L. forms was relatively low and it did not exceed 5 %. The ploidy level of the result ing plants was determined by flow-cytometric analysis. The regenerants con sisted of about equal numbers of haploids and diploids. Additionally, among plants regenerated from anthers of yellow fruit forms, two mixoploids were observed.
11

In vitro-regenerated weltand sedge Eriophorum vaginatum L. is genetically stable

71%
Rewers M., Kisiala A., Drouin J., Sliwinska E., Cholewa E.
Acta Physiologiae Plantarum
|
2012
|
tom 34
|
nr 6
Eriophorum vaginatum L. is a promising species for phytostabilization, restoration, or creation of wetlands, because it can survive in cold, nutrient-poor, or metal-contaminated soils. However, its propagation on a large scale is problematic due to the infrequent production of viable seeds, seed dormancy, and the limitations of reproduction by rhizomes. A technique to rapidly and effectively produce large quantities of outplanting stock of this species was sought. Seeds of E. vaginatum were cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators at different concentrations. The highest regeneration rate was obtained on MS medium supplemented with 2.26 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 µM kinetin (KIN) for callus induction, and 17.76 µM BA (6-benzylaminopurine) for shoot regeneration as well as when 2.26 µM 2,4-D and 4.65 µM KIN was added to the callus-induction medium, and 8.88 µM or 17.76 µM BA to the shootregeneration medium. The regenerated shoots were rooted on MS medium without growth regulators and acclimatized in a greenhouse. Genetic stability of the in vitro regenerants was determined using flow cytometry and random amplified polymorphic DNA. Cytometric analysis revealed that the nuclear DNA content was similar in all plant materials and amounted to about 0.8 pg/2C. The PCR amplification products were monomorphic in callusderived plants and similar to plants grown in a field. Lack of genome size variation and polymorphism within the regenerants indicates that the detailed E. vaginatum micropropagation protocol allows the production of a large number of genetically stable plants.
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Agromorfologiczna i molekularna ocena linii podwojonych haploidów papryki (Capsicum annuum L.)

71%
Olszewska D., Niklas-Nowak A., Kisiala A., Dzwonkowska M., Nowaczyk P.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2015
|
tom 580
Celem badań była ocena agromorfologiczna i molekularna pokoleń R3 ośmiu linii DH papryki (Capsicum annuum L.), otrzymanych w kulturach pylników mieszańców międzyodmianowych (ATZ1xPO)Fj oraz (ATZ1xCDT)Fr Opracowanie statystyczne wyników biometrycznych wykazało zróżnicowanie między badanymi formami, a także bardzo dużą jednorodność fenotypową w obrębie pojedynczej linii. Na szczególną uwagę zasługiwały linia AP10, plonująca na poziomie formy matecznej ATZ1, oraz linia AP15, która przewyższała pod tym względem obydwie formy rodzicielskie. Oceniane linie DH poddano analizie molekularnej za pomocą PCR-RAPD. Osiem starterów generowało produkty polimorficzne, co pozwoliło na rozróżnienie wszystkich ocenianych w doświadczeniu linii. Na podstawie otrzymanych elektroforogramów obliczono współczynniki dystansu genetycznego oraz opracowano dendrogramy ilustrujące zróżnicowanie genetyczne między formami rodzicielskimi i potomnymi liniami DH. Wyróżnione pod względem cech użytkowych linie AP10 i AP15 stanowiły wspólną grupę o najmniejszym współczynniku dystansu genetycznego.
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