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1

Axonal outgrowth stimulation after alginate/mesenchymal stem cell therapy in injured rat spinal cord

100%
Blasko J., Szekiova E., Slovinska L., Kafka J., Cizkova D.
Acta Neurobiologiae Experimentalis
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2017
|
tom 77
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nr 4
Despite strong efforts in the field, spinal cord trauma still belongs among the untreatable neurological conditions at present. Given the complexity of the nervous system, an effective therapy leading to complete recovery has still not been found. One of the potential tools for supporting tissue regeneration may be found in mesenchymal stem cells, which possess anti‑inflammatory and trophic factor‑producing properties. In the context of transplantations, application of degradable biomaterials which could form a supportive environment and scaffold to bridge the lesion area represents another attractive strategy. In the present study, through a combination of these two approaches we applied both alginate hydrogel biomaterial alone or allogenic transplants of MSCs isolated from bone marrow seeded in alginate biomaterial into injured rat spinal cord at three weeks after spinal cord compression performed at Th8‑9 level. Following three‑week survival, using immunohistochemistry we studied axonal growth (GAP‑43 expression) and both microglia (Iba‑1) and astrocyte (GFAP) reactions at the lesion site and in the segments below and above the lesion. To detect functional improvement, during whole survival period we performed behavioral analyses of locomotor abilities using a classical open field test (BBB score) and a Catwalk automated gait analyzing device (Noldus). We found that despite the absence of locomotor improvement, application of both alginate and MSCs caused significant increase in the number of GAP‑43 positive axons.
2

Comparison of dynamic behavior and maturation of neural multipotent cells derived from different spinal cord developmental stages: an in vitro study

86%
Slovinska L., Szekiova E., Blasko J., Devaux S., Salzet M., Cizkova D.
Acta Neurobiologiae Experimentalis
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2015
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tom 75
|
nr 1
Neural progenitor cells (NPCs) are characterized as undifferentiated cells with the ability of self-renewal and multipotency to give rise to other cells of the nervous system. In our in vitro study we demonstrate the proliferative and differentiative potential of NPCs isolated from the spinal cord at different developmental stages (embryonal, early postnatal, adult), maintained and expanded within neurospheres (NSs). Using the NSs culture system, we examined the size, number of NSs and their fate when exposed to differentiation conditions. Based on immunocytochemical analyses for cell markers (MAP 2, GFAP, RIP) we evaluated the occurrence of various cell types: neurons, astrocytes and oligodendrocytes. The results show that NSs increased in size during cultivation time via NPC proliferation, but proliferation potential decreased during maturation stages. In addition, NPCs derived from spinal cord developmentally different stages gave rise to a consistent ratio of glial and neuronal progeny (3:1), and adult tissues represent a comparable source of NPCs compared to embryonal and early postnatal tissues. These data provide useful information for large-scale in vitro expansion of NPCs required for potential cell therapy after spinal cord injury.
3

Spinal cord injury or enhanced physical activity stimulate division of ependymal cells rat spinal cord

72%
Novotna I., Slovinska L., Cizek M., Nagyova M., Rosocha J., Radonak J., Vanicky I., Cizkowa D.
Acta Neurobiologiae Experimentalis
|
2009
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tom 69
|
nr 3
Ependymal cells (EC) in the spinal cord central canal (CC) are believed to be responsible for the postnatal neurogenesis following pathological or stimulatory conditions. In the present study we have analyzed the proliferation of the CC EC in adult rats processed to spinal cord injury (SCI) or enhanced physical activity. To label dividing cells, a daily injection of Bromodeoxyuridine (BrdU) was administered over a 14 days. Quantifi cation of BrdU positive EC was performed by using stereological principles of systematic random sampling and optical Dissector software. The number of BrdU labeled EC increased gradually with the time of survival after both paradigms, SCI or increased physical activity. In the SCI group we have found 8.2-fold (7 days) and 11.3-fold (14 days) increase of proliferating EC in the rostro-caudal regions. Furthermore, the cervical spinal cord segments revealed 2 to 3-fold increase of EC for both time-points analyzed. In the second group subjected to enhanced physical activity by running wheel, we have observed 1.8 fold increase of dividing EC in the lumbar and 3.2 fold increase in the cervical spinal cord segments at 7 days, but no signifi cant progression at 14 days. This data shows, that SCI or enhanced physical activity in adult rats induces an endogenous EC response leading to their increased proliferation, which may be benefi cial for recovery of motor function. Supported by: APVV 51-002105, VEGA 2-0019-08, VEGA 1-0674-09, VEGA 1/4223/07, APVV SK-CZ-0045-07.
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