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Fruits of large-leaved lime dried to 10% may be stored for 16 years in sealed containers at –3°C without loosing seed viability. Dormancy of seeds, extracted from hard fruit coats, may be released after chemical scarification in concentrated sulphuric acid for 10 minutes, followed-by stratification without any medium (chilling) at the temperature of 3°C, for 20–24 weeks, i.e. until the first seeds start to germinate. After such pretreatment, during the germination test conducted at alternating temperatures 3~15°C (16 + 8 hours/day) seeds germinate near 90% in several weeks. For seedling production scarified and stratified seeds should be sown in early spring into trays under a plastic tunnel which ensures a high percentage of seedlings emergence. Sowing of the pretreated seeds in spring in a open nursery gives poor results.
Black alder, a major forest tree species in Europe, thrives in moist soils. During the seeds extraction (nutlets) from the cones, the heaviest seeds were observed to fall out first. Seed weight was not correlated to the ability of germination; no significant differences were observed between the heaviest and the lightest seeds. Seeds with a fresh mass moisture content of 8−9% after storage in –3°C retained their high germinability for six winters. However, seedling emergence decreased gradually when tested in cell trays in an unheat− ed greenhouse. Along with the decline of seedling emergence, the participation of high seedlings (25 cm) decreased and participation of small seedlings increased simultaneously. These results question whether the practice of storing seeds in –3°C for long time is the best method of germplasm preservation.
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Savin juniper (Juniperus sabina L.) is a relict shrub in Poland with only one natural stand in Pieniny Mts. Se- eds require warm followed by cold stratification to overcome dormancy. Application of temperature regime at 20°/3°C layout, for 14 and 17 weeks respectively, is more effective than stratification at 15°/3°C. Interruption of warm stratification phase after 7 weeks and seeds drying at room temperature for 3-days with warm stratification follow-on resulted in increasing of germination capacity from 25% to approximately 45%. Germinated seeds pricked to substrate into containers gave seedlings 3 cm high when cultivated under plastic tunnel in the first vegetation season.
Irregular seed crop in Betula pendula (Silver Birch) is a reason to create seed reserves, ensuring nursery production in years of crop failure. This study investigated the effects of seed moisture content and storage temperature on germinability and seedling emergence in container cultivation. Mature catkins were collected separately from 3 trees. The mixture of winged nuts and scales was dried to 3 levels of moisture content and stored at 3°C, –3°C and –10°C. After storage for 3, 5 and 6 years, the seeds were separated from scales and next dewinged and sorted in acetone into empty and filled seeds. Cleaned nuts were germinated in the Jacobsen germinator. Besides, to assess seedling emergence, seeds were sown on the surface of peat mixed with perlite, in multi-cell trays under a plastic tunnel. Significant differences in germinability were detected depending on the mother tree, seed moisture content and storage time. The viability of seeds stored at –10°C remained unchanged for 6 years regardless of moisture content (ca. 8–12%). Seeds stored at higher temperatures lost their germinability faster. An unexplained increase in seedling emergence was observed after extended seed storage at –10°C, in contrast to a gradual decrease in seedling emergence after extended storage at –3°C.
The observed juniper decline, lack of natural regeneration, and disappearance of numerous populations of the common juniper in Poland, were the major reasons for initiation of research on its sexual propagation and seedling production. This study shows that seed dormancy in this species is broken by warm-cold stratification at 15°C/3°C, for 14+12 weeks, respectively. Seed drying for 72 h at room temperature in the middle of the warm phase (i.e. after 4–8 weeks of stratification at 15°C) and the following cold stratification at 3°C, cause a significant increase in seed germination capacity at cyclically alternating temperatures of 3~15°C, and in seedling emergence in growing trays (67 cells each) in a greenhouse. However, seedling emergence was over 2-fold to 4-fold lower in an open nursery than in the greenhouse. After sowing in the nursery, secondary dormancy was probably induced, because some seeds germinated in the following year. Some very young, 2-year-old seedlings started to produce male or rarely female cones. This study also showed that soil conditions of mother plants can influence the pattern of seed germination and seed ling emergence.
This study was aimed to improve the understanding of germination ecology and to explain the invasive character of the common buckthorn (Rhamnus catharticus) in North America. Its fully mature seeds are characterized by a lack of dormancy. In laboratory conditions, favourable thermal conditions were identified for seed storage, germination and seedling emergence. At the cyclically alternating temperature of 20~30°C (16+8 h daily), seeds of this species showed a high germination rate within few weeks. Two other thermal variants, 3~15°C and 3~20°C (16+8 h daily), were much less effective for seed germination. However, cold stratification (at 3°C) in a moist mixture of peat and sand, lasting 4–8 weeks, caused a remarkable increase in germination rate also at 3~15°C. Seeds extracted from ripe fruits and dried (to a moisture content of about 10%) showed high germination and emergence rates after storage for 3.5 years at –3°C.
In contrast to earlier reports, in this study Seabuckthorn seeds collected from ripe fruits proved to be non-dormant. Most of them germinated quickly at high temperatures (e.g. cyclically alternating temperatures of 20~30°C daily for 16 and 8 hours, respectively). At lower temperatures (3~20°C), seeds did not germinate or germinatedslowly, but stratification at 3°C for 4–6 weeks increasedtheir germination rate to 90–100% within 2–3 weeks. Seedling emergence in the open nursery proved to be risky, even after 6–8 weeks of seed stratification, so spring sowing in the greenhouse (or plastic tunnel) is recommended.
European bladder nut has its northern limit in Poland and is legally protected. Climatic conditions, edible seeds attractive for small animals and seed dormancy to a large extent affect its restocking under natural conditions. The aim of this study was to determine conditions for seed dormancy release. Nuts dried after collection to ca 11% of moisture content (fresh weight basis) may be stored without loss of seed viability for over 1 year in a cold store at the temperature of -3oC. For the purpose of seed dormancy release nuts need to be stratified. Seed dormancy release was found highest after the application of warm-followed-by-cold stratification, first for 4-6 weeks at the temperature of 15oC or at cyclically alternating temperature of 10~20oC (24+24 h/cycle), followed by 16-18 weeks at 3oC. Seeds germinate at 3oC with the same rate as at cyclically alternating temperature of 3~15oC (16+8 h/day). Drying of nuts at room temperature to approx. 11% during the warm stratification phase (after 2 or 4 weeks) and further stratification resulted in a significant increase in seed germinability. In some seed lots scarification of nuts (dried during the warm stratification phase) contributes to a further significant increase of seed germinability.
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