Influence of minocycline and valproic acid on glial response to intracerebral hemorrhage

• Autorzy: Majak K. , Kowanski P. , Domaradzka-Pytel B. , Spodnik J.H. , Morys J.
• Języki publikacji: EN

Abstrakty

EN

Intracerebral hemorrhage (IH) is a devastating form of cerebrovascular disease. Several studies points at glial response as one of the key factors in processes related to brain damage and repair after IH. The aim of the present study was to evaluate the influence of administration of minocycline and valproic acid on glial response after IH. 56 adult male Wistar rats (290 - 410 g) were used for the study. In 48 rats IH was induced by injection of 200 µl of autologous blood into the temporal lobe structures. Next animals were divided into three groups that received either minocycline (3 × 200 mg/kg), or valproic acid (2 × 45 mg/kg) or 0.09% saline (2 ml) for 7 consecutive days. 8 sham-operated animals served as controls. Animals from each group were successively sacrificed at 2, 4, 24 and 48 weeks after the hematoma induction. The intensity of glial response was based on qualitative and semiquantitative evaluations of immunostained sections. We observed that during the course of IH glial cells exhibit time-dependent changes in morphology and intensity of staining. 2 and 4 weeks after IH induction activated forms of astro- and microglia were observed near the border of hematoma as well as neighboring structures, while 24 and 48 weeks later they were present mainly around the glial scar and in the degenerating white matter. White matter structures also contained NOGO-A-immunoreactive oligodendrocytes. Administration of minocycline or valproic acid decreased the number of activated astro- and microglial cells in the white matter of hemisphere contralateral to site of injury at all time points. The number of oligodendrocytes was influenced only by minocycline. The obtained results indicate that minocycline and valproic acid administration modifies the number of glial cells in the white matter of hemisphere contralateral to the site injury. The study was financially supported by Polish Ministry of Science and Higher Education grant nr 3419/B/P01/2008/35.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.63

Twórcy

autor

Majak K.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Kowanski P.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Domaradzka-Pytel B.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Spodnik J.H.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Morys J.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

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