Cellular lokalization and activity of matrix metalloproteinase-9 in mouse model of in situ theromboembolic stroke

• Autorzy: Gawlak M. , Szymanska M. , Michaluk P. , Kaczmarek L. , Wilczynski G.
• Języki publikacji: EN

Abstrakty

EN

Tissue plasminogen activator tPA is used for treatment of ischemic stroke patient. This thrombolytic agent resurrect blood supply to brain tissue, that is sensitive to oxygen and glucose deprivation. Administration of tPA is the only approved treatment and might be conducted up to 3 or 4.5 hours after ischemic stroke symptom onset. The benefits of it are time dependent and there is need for an improvement the timeliness of reperfusion. Some side effects of tPA is caused likely by its matrix metalloproteinase-9 (MMP-9) activation. Source and localization of MMP9 is still uncertain. This is investigated with high resolution in situ zymography. We use mouse model of in situ thromboembolic stroke and reperfusion as a clinically relevant. In this experimental set we identify oligodendrocytes and neurons but not astrocytes and microglia as a cellular source of gelatynolytic activity. Oligodendrocytes activity is most prominent. This is in opposition to literature. Activity of leukocytes is high however number of them suggest that their contribution to overall activity is negligible.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.73-74

Twórcy

autor

Gawlak M.

• Department of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Szymanska M.

• Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Michaluk P.

• Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Kaczmarek L.

• Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Wilczynski G.

• Department of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

Uwagi

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