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2015 | 18 | 4 |

Tytuł artykułu

Detection of Pentatrichomonas hominis in dogs using real - time PCR

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Trichomonadidae family is a protozoan occurring in different animal species. It inhabits the gastrointestinal and urinary tracts. P. hominis is rarely found in faecal samples of dogs, and its identification and differentiation from other trichomonads by light microscopy are difficult. Methods of molecular biology are the most effective in this case, because they confirm the presence of the specific species in animal organisms, irrespective of the protozoan form. The aim of this study was to find P. hominis in selected dog kennels in North-Eastern Poland. Forty-one faecal samples of dogs from 7 dog kennels were examined. The occurrence of P. hominis in 5 faecal samples of dogs with no symptoms of diarrhoea was the first one to be confirmed in Poland.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

18

Numer

4

Opis fizyczny

p.775-778,ref.

Twórcy

  • Department of Parasitology and Invasive Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Parasitology and Invasive Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

  • Cobo ER, Campero CM, Mariante RM, Benchimol M (2003) Ultrastructual study of a tetratrichomonad species isolated from prepucial smegma of virgin bulls. Vet Parasitol 117: 195-211.
  • Gookin JL, Stauffer SH, Levy MG (2007) Identification of Pentatrichomonas hominis in feline fecal samples by polymerase chain reaction assay. Vet Parasitol 145: 11-15.
  • Grellet A, Polak, B, Feugier A, Boucraut-Baralon C, Grandjean D, Vendewynckel L, Cian A, Meloni D, Viscogliosi E (2013) Prevalence, risk factors of infection and molecular characterization of trichomonads in puppies from French breeding kennels. Vet Parasitol 197: 418-426.
  • Kim YA, Kim HY, Cho SH, Cheun HI, Yu JR, Lee SE (2010) PCR detection and molecular characterization of Pentatrichomonas hominis from feces of dogs with diarrhea in the Republic of Korea. Korean J Parasitol 48: 9-13.
  • Levy MG, Gookin JL, PooreM, Birkenheuer AJ, Dykstra MJ, Litaker RW (2003) Tritrichomonas foetus and not Pentatrichomonas hominis is the etiologic agent of feline trichomonal diarrhea. J Parsitol 89: 99-104.
  • LiWC, Gong PT, YingM, Li JH, Yang J, LiH, Yang ZT, Y, Zhang GC, Zhang XC (2014) Pentatrichomonas hominis: first isolation from the feces of a dog with diarrhea in China. Parasitol Res 113: 1795-1801.
  • Meloni D, Mantini C, Goustille J, Desoubeaux G, Maakaroun-Vermesse Z, Chandenier J, Gantois N, Duboucher C, Fiori PL, Dei-Cas E, Doung TH, Viscogliosi E (2011) Molecular identification of Pentatrichomonas hominis in two patients with gastrointestinal symptoms. J Clin Pathol 64: 933-935.
  • Rivera WL, Lupisan AJ, Baking JM (2008) Ultrastructual study of a tetratrichomonad isolated from pig fecal samples. Parasitol Res 103: 1311-1316.
  • Tolbert MK, Leutenegger CM, Lobetti R, Birrell J, Gookin JL (2012) Species identification of trichomonads and associated coinfections in dogs with diarrhea and suspected trichomonosis. Vet Parasitol 187: 319-322.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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