Artificial neural tissue from cord blood for tissue replacement therapy of central nervous system

• Autorzy: Jurga M. , Ali H. , Forraz N. , McGuckin C.
• Języki publikacji: EN

Abstrakty

EN

Tissue engineering gives new tools for stem cell-based regenerative medicine and in vitro toxicology. Among different sources of stem cells human cord and cord blood are safe, clinically and ethically approved and easily obtainable sources of pluripotent stem cells able to differentiate into many tissues originated from 3 germ layers. Recently we showed that functional artifi cial neural tissue can be generated from pluripotent stem cells derived from freshly isolated human cord and cord blood stem cells in fully-defi ned culture conditions. We showed that cell starving and precise sequential introduction of single growth factors and morphogenes resulted in differentiation of pluripotent stem cells towards mature neurons in scaffold-based three-dimensional environment. The pluripotent stem cells decreased expression of the key pluripotency transcription factors: Oct4A, Sox2, Nanog, c-Myc, and acquired phenotypes of neuroblasts (Nestin+/GFAP+) followed by generation of mature neuronal networks (NeuN+, PSD95+, TUJ1+, S100beta). Finally we showed that cell–cell interactions within the 3D environment of artifi cial neural tissue were crucial for functional, electrical and calcium, activity of neural networks in vitro. We conclude that tissue-engineering approach is important for generation of functional neural tissue in vitro from cord and cord blood stem cells and therefore might be useful for future therapies of central nervous system.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.283

Twórcy

autor

Jurga M.

• Department of Neurobiology, Cel Therapy Research Institute, CTI-Lyon, France

autor

Ali H.

• Department of Neurobiology, Cel Therapy Research Institute, CTI-Lyon, France

autor

Forraz N.

• Department of Neurobiology, Cel Therapy Research Institute, CTI-Lyon, France

autor

McGuckin C.

• Department of Neurobiology, Cel Therapy Research Institute, CTI-Lyon, France