How the RNA isolation method can affect microRNA microarray results

• Autorzy: Podolska A. , Kaczkowski B. , Litman T. , Fredholm M. , Cirera S.
• Języki publikacji: EN

Abstrakty

EN

 The quality of RNA is crucial in gene expression experiments. RNA degradation interferes in the measurement of gene expression, and in this context, microRNA quantification can lead to an incorrect estimation. In the present study, two different RNA isolation methods were used to perform microRNA microarray analysis on porcine brain tissue. One method is a phenol-guanidine isothiocyanate-based procedure that permits isolation of total RNA. The second method, miRVana™ microRNA isolation, is column based and recovers the small RNA fraction alone. We found that microarray analyses give different results that depend on the RNA fraction used, in particular because some microRNAs appear very sensitive to the RNA isolation method. We conclude that precautions need to be taken when comparing microarray studies based on RNA isolated with different methods.

Słowa kluczowe

EN

RNA isolation; microRNA isolation; isolation method; small RNA fraction; microarray; pig; gene expression

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2011
• Tom: 58
• Numer: 4
• Opis fizyczny: p.535-540,fig.,ref.

Twórcy

autor

Podolska A.

• Department of Basic Animal and Veterinary Sciences, Section of Genetics and Bioinformatics, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

autor

Kaczkowski B.

autor

Litman T.

autor

Fredholm M.

autor

Cirera S.

Bibliografia

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