Expression of PC, PCK1, PCK2, LDHB, FBP1 and G6PC genes in the liver of cows in the transition from pregnancy to lactation

• Autorzy: Ostrowska M. , Gorka P. , Zelazowska B. , Sloniewski K. , Kowalski Z. M. , Zwierzchowski L.
• Języki publikacji: EN

Abstrakty

EN

The effect of the transition of dairy cows from pregnancy to lactation on the expression of genes encoding enzymes involved in hepatic glucose metabolism was studied. Six Holstein-Friesian heifers were used for this study. Liver samples were collected by biopsy on day 7 before expected parturition (-7) and days 3 and 21 after parturition (+3 and +21, respectively). The mRNA levels of pyruvate carboxylase(PC),phosphoenolpyruvate carboxykinase 1 (PCK1), phosphoenolpyruvate carboxykinase 2 (PCK2), lactate dehydrogenase B (LDHB),fructose-1,6-bisphosphatase 1 (FBP1) and glucose-6-phosphatase (G6PC) were measured using quantitative real-time PCR. The expression of PC and PCK2 mRNA on day 3 of lactation was significantly higher than that on day 7 before parturition (P<0.05) and slightly higher than on day 21 postpartum. The LDHB gene showed the highest expression level on day 3 of lactation, as compared with day 7 prepartum (P<0.001) and day 21 postpartum (P<0.001). No differences were shown in PCK1, FBP1 and G6PC expression levels between pregnancy and early lactation.

Słowa kluczowe

EN

gene expression; cow; dairy cow; liver; pregnancy; lactation; glucose metabolism

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2013
• Tom: 31
• Numer: 4
• Opis fizyczny: p.281-290,fig.,ref.

Twórcy

autor

Ostrowska M.

• Institute of Genetic and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland

autor

Gorka P.

• Department of Animal Nutrition and Feed Management, University of Agriculture in Krakow, al.Mickiewicza 24/28, 30-059 Krakow, Poland

autor

Zelazowska B.

• Institute of Genetic and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland

autor

Sloniewski K.

• Institute of Genetic and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland

autor

Kowalski Z. M.

• Department of Animal Nutrition and Feed Management, University of Agriculture in Krakow, al.Mickiewicza 24/28, 30-059 Krakow, Poland

autor

Zwierzchowski L.

• Institute of Genetic and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland

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