Association study of PIT1 and GHRH SNPs with economically important traits in pigs of three breeds reared in Poland

• Autorzy: Piorkowska K. , Ropka-Molik K. , Oczkowicz M. , Rozycki M. , Zukowski K.
• Języki publikacji: EN

Abstrakty

EN

Using the GLM procedure an association was analysed between PIT1 and GHRH SNPS and economically important traits in pigs of three breeds reared in Poland. Significant effect of GHRH/AluI SNP was observed on several quality traits such as water-holding capacity and meat colour (A,B and L*) in Polish Large White pigs (P<0.05), with the differences between alternative homozygotes being 8.1%, and 5% (meat colour), and 16% and 3% (WHC), respectively. With respect to the PIT1 gene polymorphism, it was found that pigs carrying AA genotype presented lower values of growth traits such as feed:gain ratio, daily feed intake and number of days on test compared to BB animals (P<0.05) as well as lower pH24 in loin and ham. In turn, heterozygous pigs (AB) had the highest level of fat and the lowest values of meat traits when compared to both homozygotes. It was concluded that polymorphisms in GHRH and PIT1 genes were not directly associated with quality and carcass traits, and likely they are linked to genetic markers localized on chromosomes 17 and 13. Therefore,further investigations should aim at thorough testing of GHRH and PIT1 loci.

Słowa kluczowe

EN

PIT1 gene; growth hormone releasing hormone; gene; pig; animal breed; polymorphism; Poland

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2013
• Tom: 31
• Numer: 4
• Opis fizyczny: p.303-314,ref.

Twórcy

autor

Piorkowska K.

• Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

autor

Ropka-Molik K.

• Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

autor

Oczkowicz M.

• Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

autor

Rozycki M.

• Department of Animal Genetic and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

autor

Zukowski K.

• Department of Animal Genetic and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

Bibliografia

• ARCHIBALD A.L., BROWN J.F., COUPERWHITE S., MCQUEEN H.A., NICHOLSON D.,HALEY C.S., et al., 1995 – The PiGMaP Consortium linkage map of the pig (Sus scrofa). Mammalian Genome 6, 157-175.
• BASKIN L.C, POMP D., 1997 – Restriction fragment length polymorphism in amplification products of the porcine growth hormone releasing hormone gene. Journal of Animal Science 75, 22-85.
• BOLER D., DILGER A., BIDNER B., CARR S., EGGERT J., DAY J., ELLIS M., MCKEITH F.,KILLEFER .J, 2010 – Ultimate pH explains variation in pork quality traits. Journal of Muscle Foods 21 (1): 119-130.
• CHO E.S., PARK D.H., KIM B.W., JUNG W.Y., KWON E.J., KIM CH.W., 2009 – Association of GHRH, H-FABP and MYOG polymorphisms with economic traits in pigs. Asian-Australian Journal of Animal Science 22(3), 307-312.
• COGAN J.D., PHILLIPS J.A., 1998a – Growth disorders caused by genetic defects in the growth hormone pathway. Advances in Pediatrics 45, 337-361.
• COGAN J.D, PHILLIPS J.A., 1998b – Growth hormone and Deficiency Disorders. Molecular Medicine 451-458.
• FRAJMAN P., MARGETA V., KRALIK G. 2008 – Candidate genes for slaughter traits in pigs.Krmiva 50 Zagreb 5, 267-273.
• FRANCO M.M., ANTUNES R.C., SILVA H.D., DE OLIVEIRA K.M., PEREIRA C.D., BIASE F.H., DE MORAIS FRANCO NUNES F., GOULART L.R., 2005a – Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands. Genetic Molecular Biology 28(1), 16-21.
• FRANCO M.M., ANTUNES R.C., SILVA H.D., GOULART L.R., 2005b – Association of PIT1, GH and GHRH polymorphisms with performance and carcass traits in Landrace pigs. Journal of Applied Genetics 46(2), 195-200.
• KENNES Y.M., MURPHY B.D., POTHIER F., PALIN M.F., 2001 – Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Animal Genetics 32(4), 215-218.
• LIU, B.H. 1998 – Statistical Genomics: Linkage, Mapping and QTL Analysis, CRC Press, Boca Raton.
• ŁYCZYŃSKI A., RUNOWSKA G., POSPIECH E., KOĆWIN PODSIADŁA M., WOJTCZAK J.,RZOSIŃSKA E., GRZEŚ B., MIKOŁAJCZAK B., IWAŃSKA E., 2009 – Estimation of selected porcine meat quality indicators on the basis of electrical conductivity measured 24 hours postslaughter.Animal Science Papers and Reports 27(1), 51-58.
• MILEWSKA W., 2006 – Production traits of Polish Large White sows kept in breeding herds in the Warmia and Mazury region in the years 1998-2002. Animal Science Papers and Reports, 24(1),103-112
• PARKS J.S., ABDUL-LATIF H., KINOSHITA E., MEACHAM L.R., PFAFFLE R.W., BROWN M.R., 1993 - Genetics of growth hormone gene expression. Hormone Research 40, 54-61.
• PIERZCHAŁA M., BLICHARSKI T., KURYŁ J., 2003 – Growth rate and carcass quality in pigs as related to genotype at loci POU1F1/RsaI (Pit1/RsaI) and GHRH/AluI. Animal Science Papers andReports 21, 159-166.
• PIERZCHAŁA M., PAREEK C.S., URBAŃSKIP., GOLUCH D., KAMYCZEK M., RÓŻYCKI M.,SMOCZYŃSKI R.,HORBAŃCZUK J.O., KURYŁJ., 2011 – Study of the differential transcription in liver tissue of growth hormone receptor (GHR), insulin-like growth factor IGF1, IGF2 and IGF1-receptor genes at different postnatal developmental ages in pig breeds. Molecular Biology Reports39 (3), 3055-3066.
• RADOVICK S., NATIONS M., DU Y., BERG L.A., WEINTRAUB D.B., WONDISFORD F.E. 1992 – A mutation in POU-homeodomain of PIT1 responsible for combined pituitary hormone deficiency.Science 257, 1115-1118.
• RÓŻYCKI M., TYRA M., 2009 – Wyniki oceny użytkowości tuczników i rzeźnej świń w stacjach kontroli, Stan Hodowli i Wyniki Oceny Świń, ISSN 0239-5096:48-50.
• TYRA, M., ŻAK. G. 2012 – Analysis of relationships between fattening and slaughter performance of pigs and the level of intramuscular fat (IMF) in longissimus dorsi muscle. Annals of Animal Science 12:169-178.
• SAŁYGA M., WALKIEWICZ A., BABICZ M., 2007 – Analiza jakości technologicznej I konsumpcyjnej mięsa świniodzików. Annales Universitatis Mariae Curie-Skłodowska, 25 (2), 7-15
• SOXHLET F., 1879 – Die gewichtsanalytische Bestimmung des Milchfettes, Polytechnisches Journal (Dingler‘s) 1879, 232, 461-465.
• STANCEKOVA K., VASICEK D., PESKOVICOVA D., BULLA J., KUBEK A., 1999 – Effect of genetic variability of the porcine pituitary-specific transcription factor (PIT1) on carcass traits in pigs. Animal Genetics 30, 313-315.
• YU T.P., TUGGLE C.K., SCHMITZ C.B., ROTHSCHILD M.F., 1995 – Association of PIT1polymorphisms with growth and carcass traits in pigs. Journal of Animal Science 73,1282-1288.