Optimization of Salmonella enteritidis recombinant heat shock protein 60 production

• Autorzy: Rainczak K. , Bajzert J. , Galli J. , Selera A. , Wieliczko A. , Borkowski J. , Stefaniak T.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to optimize conditions for producing Salmonella Enteritidis recombinant heat shock protein 60 (rHsp60). Seven Escherichia coli host strains (Rosetta, Turner, C41, C43, Origami, BL21pLys, Rosetta pLys) were transformed by a recombinant plasmid containing Hsp60 gene from Salmonella Enteritidis, and then cultured and induced by isopropyl-β-D-thiogalactopyranoside (IPTG). The highest S. Enteritidis rHsp60 yield was obtained using E. coli strain C41. Induction of this strain using IPTG allowed the yield 400 μg of S. Enteritidis Hsp60 protein/2L of culture, but by autoinduction the yield exceeded 800 μg/2L.

Słowa kluczowe

EN

HSP60 protein; Salmonella enteritidis; heat shock protein; host; Escherichia coli

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 1
• Opis fizyczny: p.145-146,fig.,ref.

Twórcy

autor

Rainczak K.

• Department of Immunology, Physiopathology and Veterinary Prevention, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw Wroclaw, Poland

autor

Bajzert J.

autor

Galli J.

autor

Selera A.

autor

Wieliczko A.

autor

Borkowski J.

autor

Stefaniak T.

Bibliografia

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