FISH mapping in cattle (Bos taurus L.) is not yet out of fashion

• Autorzy: De Lorenzi L. , Molteni L. , Parma P.
• Języki publikacji: EN

Abstrakty

EN

Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.

Słowa kluczowe

EN

cattle; cytogenetics; fluorescence in situ hybridization; genome; gene mapping; Bos taurus

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 4
• Opis fizyczny: p.497-499,fig.,ref.

Twórcy

autor

De Lorenzi L.

• Dipartimento di Scienze Animali, Milan University, Via Celoria 2, 20133 Milano, Italy

autor

Molteni L.

• Dipartimento di Scienze Animali, Milan University, Via Celoria 2, 20133 Milano, Italy

autor

Parma P.

• Dipartimento di Scienze Animali, Milan University, Via Celoria 2, 20133 Milano, Italy

Bibliografia

• Cribiu EP, Di Berardino D, Di Meo GP, Eggen A, Gallagher DS, Gustavsson I, et al. 2001. International System for Chromosome Nomenclature of Domestic Bovids (ISCNDB 2000). Cytogenet Cell Genet 92: 283-299.
• De Lorenzi L, Molteni L, De Giovanni A, Parma P, 2008. A new case of Rob (14; 17) in cattle. Cytogenet Genome Res 120: 144-146.
• Everts-van der Wind A, Kata SR, Band MR, Rebeiz M, Larkin DM, Everts RE, et al. 2004. A 1463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Res 14: 1424-1437.
• Gautier M, Laurent P, Hayes H, Eggen A, 2001. Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes. Genet Sel Evol 33: 19-200.
• Schibler L, Roig A, Mahe MF, Laurent P, Hayes H, Rodolphe F, Cribiu EP, 2006. High-resolution comparative mapping among man, cattle and mouse suggests a role for repeat sequences in mammalian genome evolution. BMC Genomics 7: 194.
• Snelling WM, Chiu R, Schein JE, Hobbs M, Abbey CA, Adelson DL, et al. 2007. A physical map of the bovine genome. Genome Biology 8: 165.
• The Bovine Genome Sequencing and Analysis Consortium, Elsik GC, Tellam LR, Worley KC, 2009. The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science 324: 522-528.