Lymphocyte subpopulations in the bitches with pyometra treated with aglepristone

• Autorzy: Jurka P. , Faundez R. , Winnicka A.
• Języki publikacji: EN

Abstrakty

EN

The purpose of the study was to compare flow cytometric and haematologic variables in dogs with spontaneous endometritis pyometra complex (EPC) treated with aglepristone to healthy controls. Peripheral blood mononuclear cells: CD3, CD4, CD8, and B lymphocytes (CD21) were analysed by flow cytometry and white blood cell count. Significant differences were observed (P≤0.01) between control (C) and study (S) group in the total number of leukocytes, monocytes and granulocyte populations (without lymphocytes) on beginning, after 7 d, and return to reference value after 14 d of the treatment. The percentage of the T-cell (CD3+) at the beginning was 47.22 ±9.64% of total lymphocytes, in contrast to B lymphocytes (CD21+) that represented the smallest percentage of 14.24 ±7.74% (P≤0.01). The percentage of the lymphocytes CD4+ was 27.42 ±5.53% and CD8+ was 25.18 ±4.36%. The percentage of CD3+ lymphocytes was increasing throughout the experiment in group C and gradually decreased in group S from 14th to 28th d of dioestrus. No differences in the number of CD3+, CD4+, CD8+, and CD21+ lymphocytes between group C and S on the 14th and 28th d of dioestrus (P≥0.05) were observed. The number of CD8+ cells in group S decreased gradually from day 14 to 28 but no statistical differences were noted. Treatment of pyometra with aglepristone decreased the number of leukocytes, monocytes, and granulocytes to referential value but statistically significant influence on the level of subpopulations of T and B lymphocytes was not observed. The results enabled to estimate for the first time the number of lymphocyte subpopulations in dioestrus in both healthy bitches as well as in those suffering from pyometra.

Słowa kluczowe

EN

lymphocyte; bitch; pyometra; treatment; aglepristone; animal disease; infectious disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 4
• Opis fizyczny: p.669-674,fig.,ref.

Twórcy

autor

Jurka P.

• Department of Small Animal Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland

autor

Faundez R.

autor

Winnicka A.

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