A new xenoma-forming microsporidium infecting intestinal tract of Atlantic bluefin tuna (Thunnus thynnus)

• Autorzy: Mladineo I. , Lovy J.
• Języki publikacji: EN

Abstrakty

EN

An undescribed species of Microsporidia Balbiani, 1882 was isolated from the muscularis mucosa of the intestinal mucosa of the reared Atlantic bluefin tuna (Thunnus thynnus). Transmission electron microscopy showed that the pyriform unikaryotic microsporidium, measuring 3.6 μm ± 0.08 in length and 1.8 μm ± 0.04 in width (inferred from TEM sections), had two layers of 14–17 coils of polar filament and a robust manubrium of the filament. No developmental stages were observed in xenoma. Phylogenetic analysis of the small subunit rRNA showed closest similarity with Kabatana spp. (88.4%), clustering the microsporidium together in a sister group with K. takedai and Kabatana sp. (JI-2008)/K. newberryi clade. While recognized Kabatana members show ovoid, rounded to pyriform spore, lack of sporophorous vesicle or xenoma, 3–10 coils of polar filament in 1–2 rows and are localized within cytoplasm of trunk muscle, the new species is markedly pyriform, xenoma forming, with many filament coils in 2 rows and parasitizing smooth intestinal muscle. Since morphological features were not typically congruent with any of Kabatana spp. so far described and molecular clustering indicated paraphyletic position within Kabatana clade, we suggest assigning described species to collective group Microsporidia, as Microsporidium milevae sp. nov., until more evidence permits potential formation of the new genus.

Słowa kluczowe

EN

Atlantic bluefin tuna; infection; intestinal tract; intracellular parasite; Kabatana; Kabatana takedai; Microsporidia; phylogenetic analysis; Thunnus thynnus; transmission electron microscopy; tuna fish

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2011
• Tom: 56
• Numer: 4
• Opis fizyczny: p.339-347,fig.,ref.

Twórcy

autor

Mladineo I.

• Institute of Oceanography and Fisheries, Laboratory of Aquaculture, POB 500 Split, 21000, Croatia

autor

Lovy J.

Bibliografia

• Baker M.D., Vossbrinck C.R., Maddox J.V., Undeen A.H. 1994. Phylogenetic relationship among Vairimorpha and Nosema species (Microspora) based on ribosomal RNA sequence data. Journal of Invertebrate Pathology, 64, 100–106.
• Baker M.D., Vossbrinck C.R., Didier E.S., Maddox J.V., Shadduck J.A. 1995. Small subunit ribosomal DNA phylogeny of various Microsporidia with emphasis on AIDS related forms. Journal of Eukaryotic Microbiology, 42, 564–570.
• Baquero E., Rubio M., Moura I.N.S., Pieniazek N.J., Jordana R. 2005. Myosporidium merluccius n. g., sp. nov. infecting muscle of commercial hake (Merluccius sp.) from fisheries near Namibia. Journal of Eukaryotic Microbiology, 52, 476–483. DOI: 10.1111/j.1550-7408.2005.00054.
• Barber I., Davies A.J., Ironside J.E., Forsgren E., Amundsen T. 2009. First record of a Kabatana sp. microsporidium infecting fish in the Atlantic Ocean. Diseases of Aquatic Organisms, 83, 145–152. DOI: 10.3354/dao02019.
• Bush A.O., Lafferty K.D., Lotz J.M., Shostak A.W. 1997. Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology, 83, 575–583.
• Casal G., Matos E., Teles-Grilo M.L., Azevedo C. 2008. A new microsporidian parasite, Potaspora morhaphis n. gen., sp. nov. (Microsporidia) infecting the teleostean fish, Potamorhaphis guianensis from the River Amazon. Morphological, ultrastructural and molecular characterization. Parasitology, 135, 1053–1064. DOI: 10.1017/S0031182008004654.
• Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791. DOI: 10.1214/ss/1063994980.
• Fischer W.M., Palmer J.D. 2005. Evidence from small-subunit ribosomal RNA sequence for a fungal origin of Microsporidia. Molecular and Phylogenic Evolution, 36, 606–622. DOI: 10.1016/j.ympev.2005.03.031.
• Franzen C. 2005. How do microsporidia invade cells? Folia Parasitologica, 52, 36–40.
• Hills D.M., Bull J.J. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 182–192.
• Ironside J.E. 2007. Multiple losses of sex within a single genus of Microsporidia. BMC Evolution Biology, 7, 48. DOI: 10.1186/1471-2148-7-48.
• Keeling P.J. 2003. Congruent evidence from α tubulin and β tubulin gene phylogenies for a zygomycete origin of microsporidia. Fungal Genetics and Biology, 38, 298–309. DOI: 10.1016/S1087-1845(02)00537-6.
• Larsson J.I.R. 1999. Identification of Microsporidia. Acta Protozoologica, 38, 161–197.
• Leiro J., Iglesias R., Parama A., Aragort W., Sanmartin M.L. 2002. PCR detection of Tetramicra brevifilum (Microspora) infection in turbot (Scophthalmus maximus L.) musculature. Parasitology, 124, 145–151. DOI: 10.1017/S0031182001001020.
• Lom J., Dyková I., Wang C.H., Lo C.F., Kou G.H. 2000. Ultrastructural justification for the transfer of Pleistophora anguillarum Hoshina, 1959 to the genus Heterosporis Schubert, 1969. Diseases of Aquatic Organisms, 43, 225–231. DOI: 10.3354/dao043225.
• Lom J., Nilsen F., Urawa S. 2001. Redescription of Microsporidium takedai (Awakura, 1974) as Kabatana takedai (Awakura, 1974) comb. n. Diseases of Aquatic Organisms, 44, 223–230. DOI: 10.3354/dao044223.
• Lom J., Nilsen F. 2003. Fish microsporidia: fine structural diversity and phylogeny. International Journal for Parasitology, 33, 107–127. DOI: 10.1016/S0020-7519(02)00252-7.
• Lom J., Dyková I. 2005. Microsporidian xenomas in fish seen in wider perspective. Folia Parasitologica, 52, 69–81.
• Lom J., Dyková I., Tonguthai K. 1999. Kabataia gen. n., a new genus proposed for Microsporidium spp. infecting trunk muscles of fish. Diseases of Aquatic Organisms, 38, 39–46. DOI: 10.3354/dao038039.
• Loubès C., Maurand J., Ormières R. 1979. étude ultrastructurale de Spraguea lophii (Doflein, 1898), microsporidie parasite de la baudroie: essai d’interpretation du dimorphisme sporal. Acta Protistologica, 15, 43–54.
• Mansour L., Prensier G., Jemaa S.B., Hassine O.K.B., Méténier G., Vivarès C.P., Cornillot E. 2005. Description of a xenoma-inducing microsporidian, Microgemma tincae sp. nov., parasite of the teleost fish Symphodus tinca from Tunisian coasts. Diseases of Aquatic Organisms, 65, 217–226. DOI: 10.3354/dao065217.
• Maurand J., Loubès C., Gasc C., Pelletier J., Barral J. 1988. Pleistophora mirandellae Vaney & Conte, 1901, a microsporidian parasite of cyprinid fish of rivers in Hérault: taxonomy and histopathology. Journal of Fish Diseases, 11, 251–258. DOI: 10.1111/j.1365-2761.1988.tb00546.
• McGourty K.R., Kinziger A.P., Hendrickson G.L., Goldsmith G.H., Casal G., Azevedo C. 2007. A new microsporidian infecting the musculature of the endangered tidewater goby (Gobiidae). Journal of Parasitology, 93, 655–660. DOI: 10.1645/GE-994R.1.
• Mladineo I. 2006. Microsporidia sp. in Atlantic bluefin tuna (Thunnus thynnus). Bulletin of the European Association of Fish Pathologists, 26, 152–155.
• Munday B.L., Sawada Y., Cribb T., Hayward C.J. 2003. Diseases of tunas, Thunnus spp. Journal of Fish Diseases, 26, 187–206. DOI: 10.1046/j.1365-2761.2003.00454.
• Miyake P.M., De la Serna J.M., Di Natale A., Farrugia A., Katavić I., Miyabe N., Tičina V. 2003. General review of bluefin tuna farming in the Mediterranean area. Collective Volumes of Scientific Papers ICCAT, 55, 114–124.
• Shukhgalter O.A. 2004. The parasite fauna of the chub mackerel (Scombridae: Scomber japonicus Houttuyn, 1782) in the central-eastern Atlantic (Atlantic coast of the Northern Africa and the Azores Archipelago banks). Parazitologiya, 38, 160–170 (In Russian).
• Shaw R.W., Kent M.L. 1999. Fish microsporidia. In: (Eds. M. Wittner and L.M. Weiss) The Microsporidia and Microsporidiosis). ASM Press, Washington DC, pp. 447–501.
• Sprague V., Becknel J.J., Hazard E.I. 1992. Taxonomy of the phylum Microsporidia. Critical Reviews in Microbiology, 18, 285–395. DOI: 10.3109/10408419209113519.
• Urawa S. 1989. Seasonal occurrence of Microsporidium takedai (Microsporidia) infection in masou salmon, Oncorhynchus masou, from Chitose river. Physiology and Ecology Japan, 1, 587–598.
• Vagelli A., Paramá A., Sanmartín M.L., Leiro J. 2005. Glugea vincentinae sp. nov. (Microsporidia: Glugeidae) infecting the Australian marine fish Vicentia conspersa (Teleostei: Apogonidae). Journal of Parasitology, 91, 152–157. DOI: 10.1645/GE-388R.
• Vávra J., Larsson R. 1999. Structure of mitochondria. In: (Eds. M. Wittner and L. Weiss) The Microsporidia and Microsporidiosis. ASM Press, Washington DC, pp. 7–84.
• Vossbrinck C.R., Maddox J.V., Friedman S., Debrunner-Vossbrinck B.A., Woese C.R. 1987. Ribosomal RNA sequence suggests microsporidia are extremely ancient eukaryotes. Nature, 326, 411–414.
• Vossbrinck C.R., Debrunner-Vossbrinck B.A. 2005. Molecular phylogeny of the Microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitologica, 52, 131–142.