Wykorzystanie techniki HRM do identyfikacji pojedynczych zmian w 3'UTR RNA polskich izolatów wirusa ToTV

• Autorzy: Wieczorek P. , Budziszewska M. , Obrebalska-Steplowska A.

Warianty tytułu

EN

Using of HRM technique for identification of single changes in 3'UTR RNA of polish isolates of ToTV virus

• Języki publikacji: PL

Abstrakty

EN

Tomato torrado virus is a member of Secoviridae family, genus Torradovirus. It is a dangerous pathogen of tomato plants causing intensive necrosis of leaves and fruits, leading to plant death, and significantly decreasing production of this vegetable. In Poland three isolates of ToTV were identified: Wal’03, Kra and Ros. On the basis of the previously performed molecular and genomic analyses distinctive genetic variability was revealed within 3`UTR region of RNA1 Kra and Wal’03 isolates. On the basis of this heterogenous region a rapid protocol of PCR-HRM reaction was developed allowing the identification and differentiation of two isolates of Tomato torrado virus as well as constituting rapid test to monitor the nucleotide point mutation within this regulatory region. Since the 3`UTR region is known to play a role in the replication process hence the featured heterogeneity might have an impact on the control of viral particles accumulation.

Słowa kluczowe

PL

identyfikacja; izolaty wirusowe; lancuchowa reakcja polimerazy; wirus nekrozy pomidora; wirusy; zmiennosc genetyczna

EN

virus identification; virus isolate; polymerase chain reaction; Tomato torrado virus; virus; genetic variability

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2010
• Tom: 50
• Numer: 3
• Opis fizyczny: s.1352-1358,tab.,wykr.,bibliogr.

Twórcy

autor

Wieczorek P.

• Międzyzakładowa Pracownia Biologii Molekularnej, Instytut Ochrony Roślin – Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań

autor

Budziszewska M.

autor

Obrebalska-Steplowska A.

Bibliografia

• Budziszewska M., Obrępalska-Stęplowska A., Wieczorek P., Pospieszny H. 2008. The nucleotide sequence of a Polish isolate of Tomato torrado virus. Virus Genes 37: 400–406.
• Pospieszny H., Borodynko N., Obrępalska-Stęplowska A., Hasiów B. 2007. The First Report of Tomat torrado virus in Poland. Plant Dis. 91, p. 1364.
• Reed G.H., Kent J.O., Wittwer C.T. 2007. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics. 6: 597–608.
• Sambrook J., Russel D. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Vol. 1. Cold Spring Harbor Laboratory Press., New York, p. 7.4–7.8.
• Sanfacon H., Wellink J., Le Gall O., Karasev A., van der Vlugt R. 2009. Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch. Virol. 154: 899–907.
• Verbeek M., Dullemans A.M., van der Heuvel J.F.J.M., Maris P.C., van der Vlugt R.A.A. 2007. Identification and characterization of tomato torrado virus, a new plant picorna-like virus from tomato. Arch. Virol. 152: 881–890.