In vitro studies of the effect of theophylline and/or N-acetylcysteine on redox homeostasis and ketogenesis in rat hepatocytes

• Autorzy: Stec M. , Kurzeja E. , Gawel S. , Wardas P. , Maciejewska=Paszek I. , Pawlowska-Goral K.
• Języki publikacji: EN

Abstrakty

EN

The examinations were conducted on hepatocvtes isolated by means of enzymatic method from the liver of three-month-old Wistar rats. The cells were incubated in medium with addition of theophylline and/or N-acetylcysteine. Significant changes in the activity of SOD, GPx, and GR in hepatocvtes incubated in the presence of the compounds in comparison with control cells demonstrated that theophylline and/or N-acetylcysteine disturb oxidative-reductive homeostasis of the cells. Changes in concentrations of ketone bodies, resulting in disturbances of acetoacetate to ß-hydroxybutyrate molar ratio, point to an unfavourable interference of theophylline into ketogenesis, which is equivalent with the disturbance of the balance between NAD⁺ and NADH+H⁺ in hepatocvtes. N-acetylcysteine simultaneously present with theophylline in incubation medium exerted a protective action on ketogenesis.

Słowa kluczowe

EN

in vitro study; theophylline; N-acetylcysteine; redox; homeostasis; ketogenesis; rat; hepatocyte; antioxidative enzyme

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 3
• Opis fizyczny: p.519-524,ref.

Twórcy

autor

Stec M.

• Department of Food and Nutrition, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland

autor

Kurzeja E.

autor

Gawel S.

autor

Wardas P.

autor

Maciejewska=Paszek I.

autor

Pawlowska-Goral K.

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