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2011 | 55 | 1 |

Tytuł artykułu

Effects of semen collection interval on fresh and frozen semen parameters in Tushin rams

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Ejaculates from three rams were collected with artificial vagina at one day (OD1) and four day intervals (FDsI). Part of the semen samples were pooled, diluted with skim milk based extender: -egg yolk (10%) -glycerol (5%), loaded into 0.25 ml straws, equilibrated, frozen, and afterwards stored in liquid nitrogen. The following spermatozoon parameters were determined in fresh and frozen-thawed semen: motility, viability, abnormality, abnormal acrosome, and membrane integrity. Semen collected at ODI had significantly higher freeze-ability compared with semen collected at FDsI for spermatozoon motility. Frozen-thawed semen collected at ODI had higher viability and membrane integrity, and lower abnormality and abnormal acrosome percentages compared with FDsI. It was concluded that in order to use cryopreservation of the Tushin ram semen, ODI instead of FDsI collection should be preferred.

Wydawca

-

Rocznik

Tom

55

Numer

1

Opis fizyczny

p.67-70,ref.

Twórcy

autor
  • Department of Reproduction and Artificial Insemination, Veterinary Faculty, Kafkas University, 36300, Pasacayiri, Kars, Turkey
autor
autor
autor

Bibliografia

  • 1. Ammann R.P., Arcbald L.F., Farin C.E., Fields M.J., Foster D.L., Garverick H.A., Inskeep E.K., Johnson L., Katz L.S., Ott T.L., Smith M.F., Tatum M., Tibary A.: Male anatomy. In: Pathways to pregnancy and parturition. Edited by Senger P.L., Washington State University, Washington, 2003, pp. 68-71.
  • 2. Ari U.C., Daskin A.: Freezing of washed Angora goat semen with extenders added bull or ram seminal plasma. Kafkas Univ Vet Fak Derg 2010, 16, 233-239.
  • 3. Ari U.C., Daskin A.: Influence of seminal plasma separation with different solutions on post-thaw sperm quality of angora male goat semen. Reprod Dom Anim 2010, 45, 76.
  • 4. Ax R.L., Dally M., Didion B.A., Lenz R.W., Love C.C., Varner D.D., Hafez B., Bellin M.E.: Semen Evaluation. In: Reproduction in Farm Animals, Edited by Hafez E.S.E. and Hafez B., revised 6th edition, Philadelphia, USA, 2000, pp. 365-375.
  • 5. Azeredo G.A., Esper C.R., Resende K.T.: Evaluation of plasma membrane integrity of frozen-thawed goat spermatozoa with or without seminal plasma. Small Rumin Res 2001, 41, 257-263.
  • 6. Bergeron A., Brindle Y., Blondin P., Manjunath P.: Milk caseins decrease the binding of the major bovine seminal plasma proteins to sperm and prevent lipid loss from the sperm membrane during sperm storage. Biol Reprod 2007, 77, 120-126.
  • 7. Borque C., Ayllon A.: Aspartate aminotransferase in ejaculates of manchego and merino rams after minimal and maximal sperm damage. Theriogenology 1996, 46, 1017-1025.
  • 8. Garner D.L., Hafez E.S.E.: Spermatozoa and seminal plasma. In: Reproduction in Farm Animals. Edited byl Hafez B. and Hafez E.S.E., 6th edition, Philadelphia, USA, 2000, p. 107.
  • 9. Gordon I.: Fixed time sheep artificial insemination In: Controlled reproduction in small ruminant. New York. USA, 1999, pp. 121-139.
  • 10. Jennings J.J., Mcweeney J.: Effect of frequent ejaculation on semen characteristics in rams. Vet Rec 1976, 98, 230- 233.
  • 11. Karaoglu M., Macit M., Emsen H.: A study on the growth properties and livability of Tushin lambs. Turk J Veil Anim Sci 2001, 25, 261-266.
  • 12. Kaya A., Aksoy M., Tekeli T.: Influence of ejaculation frequency on sperm characteristics, ionic composition and enzymatic activity of seminal plasma in rams. Small Rum Res 2002, 44, 153-158.
  • 13. Lebouf B., Restall B., Salamon S.: Production and storage of goat semen for artificial insemination. Anim Reprod Sci 2000, 62, 113-141.
  • 14. Manson I.L.: A world dictionary of livestock breed, types and varieties. C.A.B. International, WallingfordJ UK, 1996, p. 273.
  • 15. Manjunath P., Therien I.: Role of seminal plasma phospholipids-bindings proteins in sperm membrane lipid modification that occurs during capacitation. J Reprod Immunol 2002, 53, 109-119.
  • 16. Maxwell W.M.C., Watson P.F.: Recent pregress in the preservation of ram semen. Anim Reprod Sci 1996, 42, 55-65.
  • 17. Ritar A.J., Mendoza G., Salamon S., White I.G.: Frequent semen collection and sperm reserves of the male Angora goat (Capra hircus). J Reprod Fertil 1992, 95, 97-102.
  • 18. Salamon S., Maxwell W.M.C.: Frozen storage of ram semen. 1. Processing, freezing, thawing and fertility after cervical insemination. Anim Reprod Sci 1995, 37, 185-249.
  • 19. Shafer S., Holzmann A.: The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci 2000, 59, 201-211.
  • 20. Skandhan K.P., Mazumdar B.N.: Correlation of sodium and potassium in human seminal plasma with fertilizing capacity of normal, and infertile subjects. Andrologia 1981, 13, 147-154.
  • 21. Yue W., Shi L., Bai Z., Ren Y., Zhao Y.: Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of ram seminal plasma porteins and their correlation with semen characteristics. Anim Reprod Sci 2009, 116, 386-391.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.dl-catalog-d3cde3a2-97f0-47df-9bfc-7771ad2757c1
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