Genetic diversity among microsporidian isolates from the silkworm, Bombyx mori, as revealed by randomly amplified polymorphic DNA (RAPD) markers

• Autorzy: Surendra Nath B , Hassan W. , Nageswara Rao S. , Vijaya Prakash N.B. , Gupta S.K. , Madana Mohanan N. , Bajpai A.
• Języki publikacji: EN

Abstrakty

EN

Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to identify these microsporidians as different isolates. The RAPD technique may be useful in detecting sources of infection of this economically important domestic insect.

Słowa kluczowe

EN

Bombyx mori; Microsporidia; Nosema; genetic diversity; infection; mulberry silkworm; polymerase chain reaction; randomly amplified polymorphic DNA marker; silkworm

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2011
• Tom: 56
• Numer: 4
• Opis fizyczny: p.333-338,fig.,ref.

Twórcy

autor

Surendra Nath B

• Molecular Pathology Division, Seribiotech Research Laboratory, Central Silk Board, CSB Campus, Carmelram Post, Kodathi, Bangalore-560035, Karnataka, India

autor

Hassan W.

autor

Nageswara Rao S.

autor

Vijaya Prakash N.B.

autor

Gupta S.K.

autor

Madana Mohanan N.

autor

Bajpai A.

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