Activity of antioxidant enzymes and concentration of Mn, Cu, Zn and protein in porcine follicular fluid

• Autorzy: Lasota B. , Ogonski T. , Blaszczyk P. , Seremak B. , Ligocki M. , Telesinski A. , Kopczynski P.

Warianty tytułu

PL

Aktywność enzymów antyoksydacyjnych oraz koncentracja białka, Mn, Cu i Zn w świńskim płynie pęcherzykowym

• Języki publikacji: EN

Abstrakty

EN

Oxidative metabolism is essential for the gamete and the embryo energy production and is unavoidably associated with generation of reactive oxygen species (ROS). Enzymatic antioxidant defenses are present in the mammalian oocytes, embryos and follicular fluid (FF). An addition of porcine FF to maturation media have positive effects on the IVM and IVF results. The aim of this study was to study the CAT, SOD and GSH-Px activity, as well as Cu, Mn, and Zn concentration in porcine FF collected from the left and the right ovary. The ovaries were collected from 77 gilts at age 8 months. All the analyzed samples of FF revealed active enzymes (24.2·10–3 U · l–1, 2.65·10–3 U · l–1, and 525 U · l–1 for CAT, GSH-Px, and SOD, respectively) and contained Zn, Cu and Mn (13.8·103mol · l–1, 33.3·103 mol · l–1, and 133·10–9 mol · l–1, respectively). In the pFF collected from the left ovary, SOD and GSH-Px activity was higher compared to pFF from the right ovary. On the contrary, the concentration of Cu and Mn was significantly lower in the left ovary pFF. The concentration of Cu ions was negatively correlated with SOD activity. The CAT activity in pFF form left or right ovary did not show any differences.

PL

Metabolizm oksydacyjny jest niezbędny do produkcji energii na potrzeby gamet i zarodków, co związane jest z wytwarzaniem reaktywnych form tlenu. Antyoksydacyjny mechanizm ochrony enzymatycznej znajduje się w oocytach i zarodkach ssaków, a także w płynie pęcherzykowym. Dodatek świńskiego płynu pęcherzykowego do odżywek hodowlanych korzystnie wpływa na wyniki zapłodnienia i dojrzewania in vitro. Celem badań było określenie aktywności katalazy (CAT) dysmutazy ponadtlenkowej (SOD) i persoksydazy glutationowej (GSH-Px) oraz koncentracji Cu,Mn i Zn wpłynie pęcherzykowym świń pobranym z prawego i lewego jajnika. Ogółem pobrano jajniki od 77 loch w wieku 8 miesięcy.Wewszystkich analizowanych próbkach badane enzymy wykazywały aktywność (24,2; 2,65 10–3 U · l–1 i 525 U · l–1 odpowiednio w przypadku CAT, GSH-Px i SOD) i zawierały jomy Zn, Cu i Mn (odpowiednio 13,8 · 103 mol · l–1; 33,3 · 103 mol · l–1 i 133 · 10–9 mol · l–1). W płynie pęcherzykowym pobranym z lewego jajnika aktywność SOD i GSH-Px była wyższa niż w płynie pęcherzykowym prawego jajnika. Natomiast koncentracja Cu i Mn była istotnie niższa w płynie z lewego jajnika. Koncentracja jonów Cu była skorelowana ujemnie z aktywnością SOD. Aktywność CAT w płynie pęcherzykowym z prawego lub lewego jajnika nie wykazała różnic.

Słowa kluczowe

EN

enzyme activity; antioxidative enzyme; concentration; manganese concentration; copper concentration; zinc concentration; protein concentration; follicular fluid; pig; catalase; microelement; superoxide dismutase

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Zootechnica
• Rocznik: 2011
• Tom: 10
• Numer: 1
• Opis fizyczny: p.55-64,fig.,ref.

Twórcy

autor

Lasota B.

• Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology in Szczecin, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Ogonski T.

autor

Blaszczyk P.

autor

Seremak B.

autor

Ligocki M.

autor

Telesinski A.

autor

Kopczynski P.

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