Zasiedlenie nasion ekotypów traw z rodzaju Festuca i Lolium przez endofity z rodzaju Neotyphodium i Epichloe

• Autorzy: Panka D. , Zurek G.

Warianty tytułu

EN

Seed infection of grass ecotypes from Festuca and Lolium genera with Neotyphodium and Epichloe endophytes

• Języki publikacji: PL

Abstrakty

PL

Przeprowadzono badania nad występowaniem grzybów endofitycznych rodzajów Neotyphodium i Epichloë w kolekcji nasion ekotypów kostrzewy łąkowej (Festuca pratensis), kostrzewy czerwonej (F. rubra) i życicy trwałej (Lolium perenne). Przebadano łącznie 78 prób nasion pochodzących z Polski, Słowacji i Nowej Zelandii. Grzybnię endofitów wykryto w 79,5% analizowanych prób. Zasiedlenie nasion badanych gatunków traw przez endofity rodzaju Neotyphodium i Epichloë kształtowało się na bardzo wysokim poziomie i wynosiło odpowiednio: kostrzewa łąkowa - 93%, kostrzewa czerwona - 64,5% i życica trwała - 60%. Identyfikacja endofita z nasion życicy trwałej metodą PCR potwierdziła obecność Neotyphodium lolii. Długi okres przechowywania (2-4 lat) całkowicie zredukował żywotność grzybni endofitów w próbach zasiedlonych nasion i uniemo żliwił wyizolowanie tych grzybów z siewek do dalszych badań.

EN

Research on infection level of grass ecotypes with endophytes of Neotyphodium and Epichloë genera was conducted. Collection of 78 seed lots of meadow fescue (Festuca pratensis), red fescue (F. rubra) and perennial ryegrass (Lolium perenne) originated from Poland, Slovakia and New Zealand was used. Endophyte mycelium was detected in 79.5% of analyzed seed lots. Infection level of tested ecotypes was as follows: meadow fescue - 93%, red fescue - 64.5%, perennial ryegrass - 60%. Polymerase Chain Reaction assay confirmed Neotyphodium lolii presence in infected seeds of perennial ryegrass. Due to long storage period (2-4 years) there was no viable mycelium in plants growing from infected seeds. Thus, the attempts of endophyte isolation on PDA medium were unsuccessful.

Słowa kluczowe

PL

ekotypy; Epichloe; Festuca pratensis; Festuca rubra; grzyby endofityczne; izolacja; kostrzewa czerwona; kostrzewa lakowa; Lolium perenne; nasiona; Neotyphodium; trawy; zasiedlanie; zycica trwala

EN

ecotype; Epichloe; Festuca pratensis; Festuca rubra; endophytic fungi; isolation; red fescue; meadow fescue; Lolium perenne; seed; Neotyphodium; grass; fungi colonizing grass; fungi inhabiting; perennial rye-grass

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2008
• Tom: 529
• Opis fizyczny: s.131-137,tab.,bibliogr.

Twórcy

autor

Panka D.

• Katedra Fitopatologii, Uniwersytet Technologiczno-Przyrodniczy im.J.J.Śniadeckich w Bydgoszczy, ul.Ks.A.Kordeckiego 20, 85-225 Bydgoszcz

autor

Zurek G.

• Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie

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