Simple evaluation of the wound healing effect of topical ointments made from Senecio vernalis (asterales: Asteraceae) plant extract in rats

• Autorzy: Ahmadi-Noorbakhsh S. , Sarrafzadeh-Rezaei F. , Maham M. , Dalir-Naghadeh B.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to evaluate the gross effect of ointments made from Senecio vernalis extract on the healing of cutaneous wounds of rats. Thirty adult female albino rats (average weight of 250±10 g) were randomly divided into six groups. Experimental wounds were made on the dorsal thoracic region of anesthetised rats. Four groups were treated with specific ointment concentrations consisted of 3.75%, 7.5%, 15%, and 30% weight/weight Senecio vernalis ethanol extract in Eucerin™ ointment base; one group was treated by the ointment basis alone (basis) and no treatment was applied to the remaining group (control). Wound healing was assessed by computerised planimetry. Data were analysed statistically using a repeated measures mixed model. Statistical evaluation of mean wound area differentiation between groups, showed no significant changes in overall rate of the wound healing (P>0.05). However, comparisons within each group, revealed that in three groups (3.75%, 7.5%, and 15% extract concentration) a significant wound size reduction happened earlier. These results deny strong hypotheses about the desirable wound healing effects of S. vernalis contrary to other similar plants of the genus Senecio, and anti-ulcer effects of Senecio brasiliensis (a similar species to S. vernalis) in the duodenum and stomach of rats and mice.

Słowa kluczowe

EN

rat; skin; wound healing; plant extract; Senecio vernalis; Compositae; therapy

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 1
• Opis fizyczny: p.117-120,ref.

Twórcy

autor

Ahmadi-Noorbakhsh S.

• Surgery Resident, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran

autor

Sarrafzadeh-Rezaei F.

autor

Maham M.

autor

Dalir-Naghadeh B.

Bibliografia

• 1. Alarcon-Aguilar F.J., Roman-Ramos R., Jimenez-Estrada M., Reyes-Chilpa R., Gonzalez-Paredes B., Flores-Saenz J.L.: Effects of three Mexican medicinal plants (Asteraceae) on blood glucose levels in healthy mice and rabbits. J Ethnopharmacol 1997, 55, 171-177.
• 2. Andree C., Swain W.F., Page C.P., Macklin M.D., Slama J., Hatzis D., Eriksson E.: In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair. Proc Natl Acad Sci USA 1994, 91, 12188- 12192.
• 3. Bohling M.W., Henderson R.A., Swaim S.F., Kincaid S.A., Wright J.C.: Comparison of the role of the subcutaneous tissues in cutaneous wound healing in the dog and cat. Vet Surg 2006, 35, 3-14.
• 4. Brown G.L., Curtsinger L.J., White M., Mitchell R.O., Pietsch J., Nordquist R., von Fraunhofer A., Schultz G.S.: Acceleration of tensile strength of incisions treated with EGF and TGF-ß. Ann Surg 1988, 208, 788-794.
• 5. Buckley A., Davidson J.M., Kamerath C.D., Wolt T.B., Woodward S.C.: Sustained release of epidermal growth factor accelerates wound repair. Proc Natl Acad Sci USA 1985, 82, 7340-7344.
• 6. Chithra P., Sajithlal G.B., Chandrakasan G.: Influence of Aloe vera on collagen characteristics in healing dermal wounds in rats. Mol Cell Biochem 1998, 181, 71-76.
• 7. Fortin H., Vigor C., Lohezic-Le Devehat F., Robin V., Le Bosse B., Boustie J., Amoros M.: In vitro antiviral activity of thirty-six plants from La Reunion Island. Fitoterapia 2002, 73, 346-350.
• 8. Hedenqvist P., Hellebrekers L.J.: Laboratory animal analgesia, anesthesia, and euthanasia. In: Handbook of laboratory animal science, edited by Hau J.,Van Hoosier G.L., CRC Press, Boca Raton, Florida, USA, 2003, pp. 417-459.
• 9. Hedlund C.S.: Surgery of the integumentary system. In: Small animal surgery, edited by.Fossum T.W, Elsevier, Boston, USA, 2007, pp. 159-162.
• 10. Hosgood G.: Wound repair and specific tissue response to injury. In: Textbook of small animal surgery, edited by D. Slatter, Saunders, Philadelphia, USA, 2003, pp. 66-84.
• 11. Laato M., Niinikoski J., Lebel L., Gerdin B.: Stimulation of wound healing by epidermal growth factor. A dose- dependent effect. Ann Surg 1986, 203, 379-381.
• 12. Littell R.C.: SAS for mixed models. Ed. SAS Institute Inc., NC, USA, Cary, 2006.
• 13. Lynch S.E., Colvin R.B., Antoniades H.N.: Growth factors in wound healing. Single and synergistic effects on partial thickness porcine skin wounds. J Clin Invest 1989, 84, 640-646.
• 14. Mustoe T.A., Pierce G.F., Morishima C., Deuel T.F.: Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model. J Clin Invest 1991, 87, 694-703.
• 15. Perez C., Agnese A.M., Cabrera J.L.: The essential oil of Senecio graveolens (Compositae): chemical composition and antimicrobial activity tests. J Ethnopharmacol 1999, 66, 91-96.
• 16. Radostits O.M., Gay C.C., Blood D.C.: Veterinary medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goat and Horses. Harcourt Publishers Ltd, WB Saunders Co., London, 2000, pp. 1661-1664.
• 17. Rashed A.N., Afifi F.U., Disi A.M.: Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculns JVI-1. J Ethnopharmacol 2003, 88, 131-136.
• 18. Roeder E.: Medicinal plants in Europe containing pyrrolizidine alkaloids. Pharmazie 1995, 50, 83-98.
• 19. Shirwaikar A., Somashekar A.P., Udupa A.L., Udupa S.L., Somashekar S.: Wound healing studies of Arislolochia bracleolata Lam. with supportive action of antioxidant enzymes. Phytomedicine 2003, 10, 558-562.
• 20. Skaanild M.T., Friis C., Brimer L.: Interplant alkaloid variation and Senecio vernalis toxicity in cattle. Vet Hum Toxicol 2001, 43, 147-151.
• 21. Toma W., Trigo J.R., Bensuaski de Paula A.C., Monteiro Souza Brito A.R.: Modulation of gastrin and epidermal growth factor by pyrrolizidine alkaloids obtained from Senecio brasiliensis in acute and chronic induced gastric ulcers. Can J Physiol Pharmacol 2004, 82, 319-325.
• 22. Toma W., Trigo J.R., Bensuaski de Paula A.C., Monteiro Souza Brito A.R.: Preventive activity of pyrrolizidine alkaloids from Senecio brasiliensis (Asteraceae) on gastric and duodenal induced ulcer on mice and rats. J Ethnopharmacol 2004, 95, 345-351.
• 23. Uzun E., Sariyar G., Adsersen A., Karakoc B., Otuk G., Oktayoglu E., Pirildar S.: Traditional medicine in Sakarya province (Turkey) and antimicrobial activities of selected species. J Ethnopharmacol 2004, 95, 287-296.