Characterization of Fusarium isolates from rice, sugarcane and maize using RFLP-IGS

• Autorzy: Hsuan H.M. , Zakaria L. , Salleh B.

Warianty tytułu

PL

Charakterystyka izolatów Fusarium z ryżu, trzciny cukrowej i kukurydzy przy wykorzystaniu RFLP-IGS

• Języki publikacji: EN

Abstrakty

EN

Isolates of Fusarium from rice, sugarcane and maize were identified as F. verticillioides, F. sacchari, F. proliferatum F. subglutinans, F. fujikuroi and F. oxysporum. The species were then characterized by restriction analysis of intergenic spacer (RFLP-IGS) using AluI, Eco88I, RsaI and XhoI. Twenty-five haplotypes were identified among the isolates of Fusarium which indicated high levels of variations. UPGMA cluster analysis was conducted to cluster the isolates and to estimate the intraspecific and interspecific variability. Isolates of F. fujikuroi from rice were clustered together with isolates of F. proliferatum from rice and maize with a similarity value of 88–100%. Isolates of F. verticilliodes from maize and sugarcane were clustered together with a similarity value ranging from 92–100%, and two isolates from rice formed another cluster. Isolates of F. oxysporum from maize and rice were clustered together with a similarity value ranging from 87–95%. Isolates of F. subglutinans from rice and maize, and F. sacchari from rice and sugarcane were also clustered together with a similarity value of 77–100%. Based on RFLP-IGS analysis, variability was observed within and between species of Fusarium from rice, maize and sugarcane and the technique could be use to complement morphological characterization and to determine genetic relationships between the species.

PL

Izolaty Fusarium z ryżu, trzciny cukrowej i kukurydzy, określono jako F. verticillioides, F. sacchari, F. proliferatum, F. subglutinans, F. fujikuroi oraz F. oxysporum. Gatunki te scharakteryzowano przy pomocy analizy restrykcyjnej międzygenowego niekodującego odcinka genu (RLFP-IGS), wykorzystując AluI, Eco881, RsaI i Hoi. Określono dwadzieścia pięć haplotypów wśród izolatów Fusarium, co wskazywało na wysoki poziom zmienności. Przeprowadzono analizę skupień w celu pogrupowania izolatów i określenia intraspecyficznej interspocyficznej zmienności. Izolaty F. fujikuroi z ryżu zgrupowano razem z izolatami F. proliferatum z ryżu i kukurydzy wykazującymi podobieństwo 88–100%. Izolaty F. verticillioides z kukurydzy i trzciny cukrowej zgrupowano w ramach wartości podobieństwa od 92 do 100%, a dwa izolaty z ryżu tworzyły inne zgrupowanie. Izolaty F. oxysporum z kukurydzy i ryżu zgrupowano również razem, z wartością podobieństwa od 87 do 95%. Izolaty F. subglutinans z ryżu i kukurydzy i F. sacchari z ryżu i trzciny cukrowej zostały też zgrupowane razem i miały wartość podobieństwa 77–100%. W oparciu o analizę RLFP–IGS obserwowano zmienność w ramach i pomiędzy gatunkami Fusarium z ryżu, kukurydzy i trzciny cukrowej. Technika RLFP–IGS mogłaby być wykorzystywana w celu uzupełnienia charakterystyki mikroskopowej i do określenia genetycznych związków pomiędzy gatunkami.

Słowa kluczowe

EN

Fusarium; isolate; rice; sugar-cane; maize; RFLP method; Fusarium sacchari; Fusarium proliferatum; Fusarium subglutinans; Fusarium fujikuroi; Fusarium oxysporum

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2010
• Tom: 50
• Numer: 4
• Opis fizyczny: p.409-415,fig.,ref.

Twórcy

autor

Hsuan H.M.

• School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia

autor

Zakaria L.

autor

Salleh B.

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