Artificial gynogenesis in common bream (Abramis brama (L.)) induced by cold-temperature shock

• Autorzy: Kucharczyk D. , Luczynski M.J. , Szczerbowski A. , Woznicki P. , Luczynski M. , Targonska-Dietrich K. , Kujawa R.J. , Mamcarz A.

Warianty tytułu

PL

Kontrolowana gynogeneza u leszcza (Abramis brama (L.)) wywołana zastosowaniem szoku termicznego zimnego

• Języki publikacji: EN

Abstrakty

EN

Bream, Abramis brama (L.), eggs fertilized with genetically inactivated sperm (UV irradiation dose of 1920 J m-2) were exposed to thermal cold shock to produce meiotic gynogenotes. The shock was applied at one-minute intervals from 1 to 10 min after egg insemination. The temperature of the shock was 2.0 ± 0.1°C, and its duration was 45 min. The water temperature prior to the shock was 20.0°C. Eggs fertilized with genetically inactivated sperm (putative haploids) exhibited retarded and abnormal development. The yield of gynogenesis was relatively low, except for the group to which the shock was applied 1 min after fertilization (about 30% in comparison with the controls). Ninety fish from the control and gynogenetic groups were reared for ten months. The survival of the gynogenetic bream was twofold lower than that of the controls. The gynogenotes were highly variable in size and exhibited some morphological abnormalities. The sex ratios in the control groups were close to 1:1, whereas all the gynogenotes were female.

PL

Oocyty leszcza (Abramis brama (L.)) zostały zaplemnione genetycznie inaktywowanym nasieniem (promieniowanie UV; dawka 1920 J m⁻²) (rys. 1) i poddane działaniu szoku termicznego zimnego, w celu otrzymania mejotycznych gynogenotów. Szok był stosowany od 1 do 10 min (w odstępach wynoszących 1 min) od momentu aktywacji ikry. Temperatura i czas szoku wynosiły odpowiednio 2,0 ± 0,1°C i 45 min. Temperatura inkubacji ikry przed poddaniem jej działaniu szoku wynosiła 20,0°C. Ikra zaplemniona inaktywowanym genetycznie nasieniem wykazywała rozwój charakterystyczny dla osobników haploidalnych, w tym charakterystyczną deformację ciała (syndrom haploidalny). Wydajność procesu gynogenezy była relatywnie niska, z wyjątkiem grup poddanych działaniu szoku w 1 min od aktywacji (około 30% w porównaniu z kontrolą) (rys. 2). 90 ryb gynogenetycznych i kontrolnych było podchowywanych przez trzy miesiące. W tym okresie przeżywalność gynogenotów była dwukrotnie niższa niż ryb kontrolnych (tab. 1). Ryby gynogenetyczne wykazywały znacznie większe zróżnicowanie w rozmiarach ciała (rys. 3) niż ryby kontrolne. Stosunek płci u ryb kontrolnych wynosił w przybliżeniu 1:1, podczas gdy wszystkie gynogenetyczne ryby okazały się samicami (rys. 4).

Słowa kluczowe

EN

artificial gynogenesis; gynogenesis; common bream; Abramis brama; cold shock; thermal shock; sex determination; genetic marker

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2004
• Tom: 12
• Numer: 2
• Opis fizyczny: p.133-143,fig.,ref.

Twórcy

autor

Kucharczyk D.

• Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Luczynski M.J.

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Poland

autor

Szczerbowski A.

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Poland

autor

Woznicki P.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Luczynski M.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Targonska-Dietrich K.

• Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Kujawa R.J.

• Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Mamcarz A.

• Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

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