Diagnostyka molekularna polskich populacji niszczyka zjadliwego (Ditylenchus dipsaci)

• Autorzy: Kierzek D. , Dobosz R. , Obrepalska-Steplowska A.

Warianty tytułu

EN

Molecular diagnostic of polish population of Ditylenchus dipsaci

• Języki publikacji: PL

Abstrakty

EN

Ditylenchus is a serious pathogen occurring worldwide which causes huge losses in agricultural crop production. The quarantine Ditylenchus dipsaci is one of the most devastating species of this genus. A proper identification by this species is crucial because of significant morphological similarity to non-quarantine Ditylenchus species. There are several diagnostic methods recommended by EPPO. However, because of considerable genetic variability within discussed species not all of them are suitable for each population. In our work some of molecular detection protocols for identtification of Polish D. dipsaci populations were tested. The most useful among proposed EPPO methods were chosen for identification of Polish population of this species. Moreover, the new specific PCR protocol was described. This method can be used both for identification of Polish population of D. dipsaci and on the basis of the amplified rDNA region the genetic correlation and phylogenetic studies can be performed.

Słowa kluczowe

PL

diagnostyka molekularna; Ditylenchus dipsaci; lancuchowa reakcja polimerazy; niszczyk zjadliwy; populacje polskie; startery; szkodniki roslin

EN

molecular diagnostics; Ditylenchus dipsaci; polymerase chain reaction; stem nematode; Polish population; starter; plant pest

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2010
• Tom: 50
• Numer: 3
• Opis fizyczny: s.1265-1269,tab.,fot.,bibliogr.

Twórcy

autor

Kierzek D.

• Instytut Ochrony Roślin – Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań

autor

Dobosz R.

autor

Obrepalska-Steplowska A.

Bibliografia

• Esquibet M., Grenier E., Plantard O., Andaloussi A., Caubel D. 2003. DNA polymorphism in the stem nematode Ditylenchus dipsaci: Development of diagnostic markers for normal and giant reces. Genome 46: 1077–1083.
• Wendt K., Vrain T., Webster J. 1993. Separation of three species of Ditylenchus and some host races od D. dipsaci by restriction fragment length polymorphism. J. Nematol. 25: 555–563.
• Marek M., Zouhar M., Rysanek P., Havranek P. 2005. Analysis of ITS sequences of nuclear rDNA and development of a PCR-based assay for the rapid identyfication of the stem nematode Ditylenchus dipsaci (Nematoda: Anguinidae) in plant tissues. Helminthologia 42: 49–56.
• Sturhan D., Brzeski M. 1991. Stem and bulb nematodes, Ditylenchus spp. p. 423–465. In: ,,Manual of Agricultural Nematology” (W.R. Nickle, ed.). Marcel Dekker, Inc., New York, 1025 pp.
• Kerkoud M., Esquibet M., Plantard O., Avrillon M., Guimier C., Franck M., Franck M., Léchappé J., Mathis R. 2007. Identyfication of Ditylenchus species associated with Fabaceae seeds based on a specific polymerase chain reaction of ribosomal DNA-ITS regions. Eur. J. Plant Pathol. 118: 323–332.