Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

• Autorzy: Serrano J. , Cipak A. , Boada J. , Gonzalo H. , Cacabelos D. , Cassanye A. , Pamplona R. , Zarkovic N. , Portero-Otin M.
• Języki publikacji: EN

Abstrakty

EN

 A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 μM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.

Słowa kluczowe

EN

gallic acid; interaction; peroxidase; man; endothelial cell; microvascular endothelial cell; antioxidant; dinitrophenyl; human endothelial cell culture; horseradish peroxidase; lactate dehydrogenase; low-density lipoprotein; malondialdehyde-lysine; myeloperoxidase; phosphate-buffered saline

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 2
• Opis fizyczny: p.193-198,fig.,ref.

Twórcy

autor

Serrano J.

• Department of Experimental Medicine, University of Lleida-IRB Lleida, Lleida, Spain

autor

Cipak A.

autor

Boada J.

autor

Gonzalo H.

autor

Cacabelos D.

autor

Cassanye A.

autor

Pamplona R.

autor

Zarkovic N.

autor

Portero-Otin M.

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