The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus

• Autorzy: Kovalenko T.N. , Ushakova G.A. , Osadchenko I. , Skibo G. , Pierzynowski S.G.
• Języki publikacji: EN

Abstrakty

EN

In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology.

Słowa kluczowe

EN

neuroprotective effect; 2-oxoglutarate; ischaemia; hippocampus; Mongolian gerbil; carotid artery; brain; glial fibrillary acidic protein; neural cell; adhesion molecule; brain ischaemia; cerebral function; blood flow

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2011
• Tom: 62
• Numer: 2
• Opis fizyczny: p.239-246,fig.,ref.

Twórcy

autor

Kovalenko T.N.

• Department of Cytology, Bogomoletz Institute of Physiology, Kiev, Ukraine

autor

Ushakova G.A.

autor

Osadchenko I.

autor

Skibo G.

autor

Pierzynowski S.G.