PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 55 | 1 |

Tytuł artykułu

Identification of genes encoding classical staphylococcal enterotoxins in Staphylococcus aureus isolated from raw milk

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This study describes a multiplex PCR assay developed for the detection of Staphylococcus aureus enterotoxin types: SEA, SEB, SEC, SED, and SEE presented in the literature as classical. The method was then used to analyse the presence of genes encoding these enterotoxins in .S aureus strains isolated from raw milk. A total of 237 raw milk samples were used in the study and 77 (32.5%) of them were found to be contaminated with S. aureus. Among them, five isolates were harbouring the genes encoding staphylococcal enterotoxins - type C (three strains) and type A (two strains). These results show that raw milk can potentially be a source of staphylococcal food poisoning.

Wydawca

-

Rocznik

Tom

55

Numer

1

Opis fizyczny

p.55-58,fig.,ref.

Twórcy

  • Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor

Bibliografia

  • 1. Asao T., Kumeda Y., Kawai T., Shibata T., Oda H., Haruki K., Nakazawa H., Kozaki S.: An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milk. Epidemiol Infect 2003, 130, 33-40.
  • 2. Bayles K.W., Iandolo J.J.: Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D. J Bacteriol 1989, 171, 4799-4806.
  • 3. Bergdoll M.S.: Staphylococcal food poisoning. In: Foodborne Diseases. Edited by Cliver D.O., Academic Press, San Diego, USA, 1990, pp. 85-106.
  • 4. Betley M.J., Mekalanos J.J.: Nucleotide sequence of the type A staphylococcal enterotoxin gene. J Bacteriol 1988, 170, 34-41.
  • 5. Bohach G.A., Schlievert P.M.: Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins. Mol Gen Genet 1987, 209, 15-20.
  • 6. Bystroń J., Bania J., Lis E., Molenda J., Bednarski M.: Characterisation of Staphylococcus aureus strains isolated from cow's milk. Bull Vet Inst Pulawy 2009, 53, 59-63.
  • 7. Bystroń J., Molenda J., Bania J., Czerw M.: Enterotoxigenicity of staphylococci isolated from some foodstuffs of animal origin. Acta Sci Pol Medicina Vet 2005, 4, 95-103.
  • 8. Bystroń J., Molenda J., Bania J., Kosek-Paszkowska K., Czerw M.: Occurrence of enterotoxigenic strains of Staphylococcus aureus in raw poultry meat Pol J Vet Sci 2005, 8, 37-40.
  • 9. Couch J.L., Soltis M.T., Betley M.J.: Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene. J Bacteriol 1988, 170, 2954-2960.
  • 10. Cremonesi P., Luzzana M., Brasca M., Morandi S., Lodi R., Vimercati C., Angellini D., Caramenti G., Moroni P., Castiglioni B.: Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Mol Cell Probes 2005, 19, 299-305.
  • 11. Cremonesi P., Perez G., Pisoni G., Moroni P., Morandi S., Luzzana M., Brasca M., Castiglioni B.: Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese. Lett Appl Microbiol 2007, 45, 586-591.
  • 12. da Silva E.R., do Carmo L.S., da Silva N.: Detection of enterotoxins A, B and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds. Vet Microbiol 2005, 106, 103-107.
  • 13. Jones C.L., Khan S.A.: Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus. J Bacteriol 1986, 166, 29-33.
  • 14. Jorgensen H.J., Mork T., Caugant D.A., Kearns A., Rorvik L.M.: Genetic variation among Staphylococcus aureus strains from Norwegian bulk milk. Appl Env Microbiol 2005, 71, 8352-8361.
  • 15. Katsuda K., Hata E., Kobayashi H., Kohmoto M., Kawashima K., Tsunemitsu H., Eguchi M.: Molecular typing of Staphylococcus aureus isolated from bovine mastitic milk on the basis of toxin genes and coagulase gene polymorphisms, Vet Microbiol 2005, 105, 301-305.
  • 16. Kerouanton A., Hennekinne J.A., Letertre C., Petit L., Chesneau O., Brisabois A., De Buyser M.L.: Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France. Int J Food Microbiol 2007, 115, 369-375.
  • 17. Loncarevic S., Jorgensen H.J., Lovseth A., Mathisen T., Rorvik L.M.: Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products. J Appl Microbiol 2004, 98, 344-350.
  • 18. Martin M.C., Fueyo J.M., Gonzales-Hevia J.M., Mendoza M.C.: Genetic procedure for identification of enterotoxigenic strains of Staphylococcus aureus from three food poisoning outbreaks. J Food Microbiol 2004, 94, 279-286.
  • 19. McKlauchlin J., Narayann G.L., Mithani V., Oneill G.: The detection of enterotoxin and toxic shock syndrome toxin genes in S. aureus by PCR. J Food Protect 1999, 63, 479-488.
  • 20. Morandi S., Brasca M., Lodi R., Cremonesi P., Castiglioni B.: Detection of classical enterotoxins and identification of enterotoxin genes in Staphylococcus aureus from milk and dairy products. Vet Microbiol 2007, 124, 66-72.
  • 21. Orden J.A., Goyache J., Hernandez J., Domenech A., Suarez G., Gomez-Lucia E.: Detection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot. J Appl Bacteriol 1992, 28, 947-950.
  • 22. Peles F., Wagner M., Varga L., Hein I., Rieck P., Gutser K., Kereszturi P., Kardos G., Turesanyi I., Beri B., Szabo A.: Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary. Int J Food Microbiol 2007, 118, 186-193.
  • 23. Pinto B., Chenoll E., Aznar R.: Identification and typing of food-borne Staphylococcus aureus by PCR based techniques. Syst Appl Microbiol 2005, 28, 340-352.
  • 24. Rall V.L.M., Vieira F.P., Rall R., Vieitis R.L., Fernandes Jr. A., Candeias J.M.G., Cardoso K.F.G., Araujo Jr. J.P.: PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk. Vet Microbiol 2008, 132, 408-413.
  • 25. Rosec J.P., Gigaud O.: Staphylococcal enterotoxin genes of classical and new types detected by PCR in France. Int J Food Microbiol 2002, 77, 61-70.
  • 26. Scherrer D., Corti S., Muehlherr J.E., Zweifel C., Stephan R.: Phenotypic and genotypie characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet Microbiol 2004, 101, 101-107.
  • 27. Tamarapu S., McKillip J.L., Drake M.: Development of a multiplex polymerase chain reaction assay for detection and differentiation of Staphylococcus aureus in dairy products. J Food Protect 2001, 64, 664-668.
  • 28. Tirado C., Schmidt K.: WHO surveillance programme for control of food-borne infections and intoxications: preliminary results and trends across greater Europe. J Infect 2001, 43, 80-84.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.dl-catalog-6e075cbb-2a1a-4156-9f98-6b32c1e671c5
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.