TRPC expression in mesenchymal stem cells

• Autorzy: Torossian F. , Bisson A. , Vannier J.-P. , Boyer O. , Lamacz M.
• Języki publikacji: EN

Abstrakty

EN

Transient receptor potential canonical (TRPC) channels are key players in calcium homeostasis and various regulatory processes in cell biology. Little is currently known about the TRPC subfamily members in mesenchymal stem cells (MSC), where they could play a role in cell proliferation. We report on the presence of TRPC1, 2, 4 and 6 mRNAs in MSC. Western blot and immunofluorescence staining indicate a membrane and intracellular distribution of TRPC1. Furthermore, the decrease in the level of TRPC1 protein caused by RNA interference is accompanied by the downregulation of cell proliferation. These results indicate that MSC express TRPC1, 2, 4 and 6 mRNA and that TRPC1 may play a role in stem cell proliferation.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2010
• Tom: 15
• Numer: 4
• Opis fizyczny: p.600-610,fig.,ref.

Twórcy

autor

Torossian F.

• Inserm, U905, University of Rouen, IFRMP, Institute for Biomedical Research, Rouen, France

autor

Bisson A.

autor

Vannier J.-P.

autor

Boyer O.

autor

Lamacz M.

Bibliografia

• 1. Lepski, G., Jannes, C.E., Maciaczyk, J., Papazoglou, A., Mehlhorn, A.T., Kaiser, S., Teixeira, M.J., Marie, S.K., Bischofberger, J. and Nikkhah, G. Limited Ca2+ and PKA-pathway dependent neurogenic differentiation of human adult mesenchymal stem cells as compared to fetal neuronal stem cells. Exp. Cell Res. 316 (2010) 216-231.
• 2. Meldolesi, J. Inhibition of adipogenesis: a new job for the ER Ca2+ pool. J. Cell Biol. 182 (2008) 11-13.
• 3. Wang, L., Singh, M., Bonewald, L.F. and Detamore, M.S. Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering. J. Tissue Eng. Regen. Med. 3 (2009) 398-404.
• 4. Ng, K.M., Lee, Y.K., Chan, Y.C., Lai, W.H., Fung, M.L., Li, R.A., Siu, C.W. and Tse, H.F. Exogenous expression of HIF-1 alpha promotes cardiac differentiation of embryonic stem cells. J. Mol. Cell. Cardiol. 48 (2010) 1129-1137.
• 5. Lepage, P.K., Lussier, M.P., McDuff, F.O., Lavigne, P. and Boulay, G. The self-association of two N-terminal interaction domains plays an important role in the tetramerization of TRPC4. Cell Calcium 45 (2009) 251-259.
• 6. Clapham, D.E., Julius, D., Montell, C. and Schultz, G. International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels. Pharmacol. Rev. 57 (2005) 427-450.
• 7. Ong, H.L., Cheng, K.T., Liu, X., Bandyopadhyay, B.C., Paria, B.C., Soboloff, J., Pani, B., Gwack, Y., Srikanth, S., Singh, B.B., Gill, D.L. and Ambudkar, I.S. Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components. J. Biol. Chem. 282 (2007) 9105-9116.
• 8. Tong, Q., Chu, X., Cheung, J.Y., Conrad, K., Stahl, R., Barber, D.L., Mignery, G. and Miller, B.A. Erythropoietin-modulated calcium influx through TRPC2 is mediated by phospholipase Cgamma and IP3R. Am. J. Physiol. Cell Physiol. 287 (2004) C1667-1678.
• 9. Alfonso, S., Benito, O., Alicia, S., Angelica, Z., Patricia, G., Diana, K. and Vaca, L. Regulation of the cellular localization and function of human transient receptor potential channel 1 by other members of the TRPC family. Cell Calcium 43 (2008) 375-387.
• 10. Abramowitz, J. and Birnbaumer, L. Physiology and pathophysiology of canonical transient receptor potential channels. FASEB J. 23 (2009) 297-328.
• 11. Riccio, A., Medhurst, A.D., Mattei, C., Kelsell, R.E., Calver, A.R., Randall, A.D., Benham, C.D. and Pangalos, M.N. mRNA distribution analysis of human TRPC family in CNS and peripheral tissues. Brain Res. Mol. Brain Res. 109 (2002) 95-104.
• 12. Lucas, P., Ukhanov, K., Leinders-Zufall, T. and Zufall, F. A diacylglycerolgated cation channel in vomeronasal neuron dendrites is impaired in TRPC2 mutant mice: mechanism of pheromone transduction. Neuron 40 (2003) 551-561.
• 13. Stowers, L., Holy, T.E., Meister, M., Dulac, C. and Koentges, G. Loss of sex discrimination and male-male aggression in mice deficient for TRP2. Science 295 (2002) 1493-1500.
• 14. den Dekker, E., Molin, D.G., Breikers, G., van Oerle, R., Akkerman, J.W., van Eys, G.J. and Heemskerk, J.W. Expression of transient receptor potential mRNA isoforms and Ca(2+) influx in differentiating human stem cells and platelets. Biochim. Biophys. Acta 1539 (2001) 243-255.
• 15. Louis, M., Zanou, N., Van Schoor, M. and Gailly, P. TRPC1 regulates skeletal myoblast migration and differentiation. J. Cell Sci. 121 (2008) 3951-3959.
• 16. Reyes, M., Lund, T., Lenvik, T., Aguiar, D., Koodie, L. and Verfaillie, C.M. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 98 (2001) 2615-2625.
• 17. Lee, C.H., Rahimian, R., Szado, T., Sandhu, J., Poburko, D., Behra, T., Chan, L. and van Breemen, C. Sequential opening of IP(3)-sensitive Ca(2+) channels and SOC during alpha-adrenergic activation of rabbit vena cava. Am. J. Physiol. Heart Circ. Physiol. 282 (2002) H1768-1777.
• 18. Chu, X., Cheung, J.Y., Barber, D.L., Birnbaumer, L., Rothblum, L.I., Conrad, K., Abrasonis, V., Chan, Y.M., Stahl, R., Carey, D.J. and Miller, B.A. Erythropoietin modulates calcium influx through TRPC2. J. Biol. Chem. 277 (2002) 34375-34382.
• 19. Yu, Y., Sweeney, M., Zhang, S., Platoshyn, O., Landsberg, J., Rothman, A. and Yuan, J.X. PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression. Am. J. Physiol. Cell Physiol. 284 (2003) C316-330.
• 20. Niwa, H., Miyazaki, J. and Smith, A.G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24 (2000) 372-376.
• 21. Schaefer, M., Plant, T.D., Stresow, N., Albrecht, N. and Schultz, G. Functional differences between TRPC4 splice variants. J. Biol. Chem. 277 (2002) 3752-3759.
• 22. Hofmann, T., Schaefer, M., Schultz, G. and Gudermann, T. Cloning, expression and subcellular localization of two novel splice variants of mouse transient receptor potential channel 2. Biochem. J. 351 (2000) 115-122.
• 23. Chu, X., Tong, Q., Cheung, J.Y., Wozney, J., Conrad, K., Mazack, V., Zhang, W., Stahl, R., Barber, D.L. and Miller, B.A. Interaction of TRPC2 and TRPC6 in erythropoietin modulation of calcium influx. J. Biol. Chem. 279 (2004) 10514-10522.
• 24. Ambudkar, I.S., Ong, H.L., Liu, X., Bandyopadhyay, B.C. and Cheng, K.T. TRPC1: the link between functionally distinct store-operated calcium channels. Cell Calcium 42 (2007) 213-223.
• 25. Chen, J., Crossland, R.F., Noorani, M.M. and Marrelli, S.P. Inhibition of TRPC1/TRPC3 by PKG contributes to NO-mediated vasorelaxation. Am. J. Physiol. Heart Circ. Physiol. 297 (2009) H417-424.
• 26. Wang, W., O'Connell, B., Dykeman, R., Sakai, T., Delporte, C., Swaim, W., Zhu, X., Birnbaumer, L. and Ambudkar, I.S. Cloning of Trp1beta isoform from rat brain: immunodetection and localization of the endogenous Trp1 protein. Am. J. Physiol. 276 (1999) C969-979.
• 27. Vag, J., Byrne, E.M., Hughes, D.H., Hoffman, M., Ambudkar, I., Maguire, P. and O'Connell, B.C. Morphological and functional differentiation of HSG cells: role of extracellular matrix and trpc 1. J. Cell Physiol. 212 (2007) 416-423.
• 28. Sours, S., Du, J., Chu, S., Ding, M., Zhou, X.J. and Ma, R. Expression of canonical transient receptor potential (TRPC) proteins in human glomerular mesangial cells. Am. J. Physiol. Renal Physiol. 290 (2006) F1507-1515.
• 29. El Boustany, C., Bidaux, G., Enfissi, A., Delcourt, P., Prevarskaya, N. and Capiod, T. Capacitative calcium entry and transient receptor potential canonical 6 expression control human hepatoma cell proliferation. Hepatology 47 (2008) 2068-2077.
• 30. Mori, Y., Wakamori, M., Miyakawa, T., Hermosura, M., Hara, Y., Nishida, M., Hirose, K., Mizushima, A., Kurosaki, M., Mori, E., Gotoh, K., Okada, T., Fleig, A., Penner, R., Iino, M. and Kurosaki, T. Transient receptor potential 1 regulates capacitative Ca(2+) entry and Ca(2+) release from endoplasmic reticulum in B lymphocytes. J. Exp. Med. 195 (2002) 673-681.
• 31. El Hiani, Y., Ahidouch, A., Lehen'kyi, V., Hague, F., Gouilleux, F., Mentaverri, R., Kamel, S., Lassoued, K., Brule, G. and Ouadid-Ahidouch, H. Extracellular signal-regulated kinases 1 and 2 and TRPC1 channels are required for calcium-sensing receptor-stimulated MCF-7 breast cancer cell proliferation. Cell. Physiol. Biochem. 23 (2009) 335-346.
• 32. Labelle, D., Jumarie, C. and Moreau, R. Capacitative calcium entry and proliferation of human osteoblast-like MG-63 cells. Cell Prolif. 40 (2007) 866-884.

Uwagi

PL

Rekord w opracowaniu.