Comparison of multilocus variable-number tandem-repeat analysis with multilocus sequence typing and pulsed-field gel electrophoresis for Enterococcus faecalis

• Autorzy: Sadowy E. , Sienko A. , Hryniewicz W.
• Języki publikacji: EN

Abstrakty

EN

Enterococcus faecalis represents recently an important etiological agent of health care-associated infections (HAIs) and there is a need for evaluation and comparison of typing methods available for this microorganism. We tested multilocus VNTR (variable-number tandem repeats) analysis (MLVA) on a well-characterized collection of 153 clinical isolates of E. faecalis, corresponding to 52 multilocus sequence types and 67 pulsed-field gel electrophoresis (PFGE) profiles. MLVA showed high discriminatory power, discerning 111 different types (diversity index equal 98.9%). The concordance MLVA/MLST and MLVA/PFGE was 0.95 and 0.74, respectively. High discriminatory power of MLVA indicates its utility for local epidemiology such as outbreak investigation, and for differentiation of clones defined by other methods.

Słowa kluczowe

EN

variable-number tandem-repeat analysis; multilocus sequence typing; pulsed-field gel electrophoresis; Enterococcus faecalis; gastrointestinal tract; etiological agent; epidemiology; clinical isolate; Poland

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 4
• Opis fizyczny: p.335-339,fig.,ref.

Twórcy

autor

Sadowy E.

• Department of Molecular Microbiology, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw, Poland

autor

Sienko A.

autor

Hryniewicz W.

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