Five novel SNPs of the bovine LHX4 gene and their association with growth traits in native Chinese cattle breeds

• Autorzy: Liu J.X. , Ren G. , Chen H. , Li F. , Lan X. Y. , Li M. J. , Wang J. Q.
• Języki publikacji: EN

Abstrakty

EN

LHX4 is a LIM homeodomain transcription factor involved in pituitary ontogenesis. Some mutations of LHX4 are associated with combined pituitary hormone deficiency that affects growth and development in animal. The objective of this study is revealing to these polymorphisms of in LHX4 gene exons 1, 2, 3 in 820 Chinese cattle and to assess possible association of polymorphisms in LHX4 gene with growth traits in NY breed. The PCR-SSCP and DNA sequencing of LHX4 gene showed five novel synonymous mutations: (GenBank: NW_001493442.2: g.35143G >A, g.35152C>T, g.35212C>T, g.35230G>A, g.35233T>C). Genotype MM (GG-CC-CC-GG-TT) and haplotype M (G-C-C-G-T) were dominant in the four breeds, and genotype frequencies of LHX4 in the four cattle populations agreed with Hardy-Weinberg equilibrium (P>0.05), although their frequencies significantly differed among the four analyzed populations (***P<0.001). The association analysis showed that individuals with genotype MM had greater body weight than those with genotype MN (*P<0.05) at eighteen months of age in Nanyang cattle.

Słowa kluczowe

EN

cattle; bovine gene; LHX4 gene; growth trait; Chinese breed; animal breed

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2011
• Tom: 29
• Numer: 1
• Opis fizyczny: p.19-28,fig.,ref.

Twórcy

autor

Liu J.X.

• Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest AandF University, Yangling, Shaanxi, 712100, China

autor

Ren G.

autor

Chen H.

autor

Li F.

autor

Lan X. Y.

autor

Li M. J.

autor

Wang J. Q.

Bibliografia

• Botstein D., White R.L., Skolnick M., Davis R.W. 1980 − Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32, 314-331.
• Castinetti F., Reynaud R., Saveanu A., Quentien M.H., Albarel F., Barlier A., Enjalbert A., Brue T., 2008a − Clinical and genetic aspects of combined pituitary hormone deficiencies. Annales d’Endocrinologie 69, 7-17.
• Castinetti F., Saveanu A., Reynaud R., Quentien M.H., Buffin A., Brauner R., Kaffel N., Albarel F., Guedj A.M., Kholy M.El., Amin M., Enjalbert A.,Barlier A., Brue T., 2008b − A novel dysfunctional LHX4 mutation with high phenotypical variability in patients with hypopituitarism. Journal of Clinical Endocrinology & Metabolism 93,2790-2799.
• Dattani M.T., 2004 − Novel insights into the aetiology and pathogenesis of hypopituitarism.Hormone Research 62, 1-13.
• Gan Q.F., Zhang L.P., Li J.Y., Hou G.Y., Li H.D., Gao X., Ren Y.H., Chen J.B., Xu S.Z., 2008 − Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. Journal of Applied Genetics 49, 251-255.
• Komar A.A., 2007 − Silent SNPs: impact on gene function and phenotype. Pharmacogenomics 8, 1075-1080.
• Lan X.Y., Pan C.Y., Chen H., Zhang L.Z., Zhao M., Zhang B., Zhang C.L., Wang J.Q., 2007 − DdeI Polymorphism in Coding Region of Goat POU1F1 Gene and Its Association with Production Traits. Asian-Australasian Journal of Animal Sciences 20, 1342-1348.
• Li H., Witte D.P., Branford W.W., Aronow B.J., Weinstein M., Kaur S., Singh G., Schreiner C.M., Whitsett J.A., Scott W.J., Potter S.S., 1994 − Gsh-4 encodes an LIM-type homeodomain is expressed in the developing central nervous system and is required for early postnatal survival. Embo Journal 13, 2876-2885.
• Machinis K., Pantel J., Netchine I., Leger J., Camand O.J.A., Sobrier M.L.,Dastot -Le Moal F., Duquesnoy P., Abitbol M., Czernichow P., Amselem S.,2001 − Syndromic short stature in patients with a germline mutation in the LIM homeobox LHX4.American Journal of Human Genetics 69, 961-968.
• Machinis K., Amselem S., 2005 − Functional relationship between LHX4 and POU1F1 in light of the LHX4 mutation identified in patients with pituitary defects. Journal of Clinical Endocrinology & Metabolism 90, 5456-5462.
• Mehta A., Dattani M.T., 2008 − Developmental disorders of the hypothalamus and pituitary gland associated with congenital hypopituitarism. Best Practice & Research Clinical Endocrinology & Metabolism 22, 191-206.
• Mullen R.D., Colvin S.C., Hunter C.S., Savage J.J., Walvoord E.C., Bhangoo A.P.S., Ten S., Weigel J., Pfaffle R.W., Rhodes S.J., 2008 − Roles of the LHX3 and LHX4 LIM-homeodomain factors in pituitary development. Molecular and Cellular Endocrinology 265,190-195.
• Oh J.J., Koegel A.K., Phan D.T., Razfar A., Slamon D.J., 2007 − The two single nucleotide polymorphisms in the H37/RBM5 tumour suppressor gene at 3p21.3 correlated with different subtypes of non-small cell lung cancers. Lung Cancer 58, 7-14.
• Pfaeffle R.W., Hunter C.S., Savage J.J., Duran -Prado M., Mullen R.D., Neeb Z.P., Eiholzer U., Hesse V., Haddad N.G., Stobbe H.M., Blum W.F., Weigel J.F.W.,Rhodes S.J., 2008 − Three novel missense mutations within the LHX4 gene are associated with variable pituitary hormone deficiencies. Journal of Clinical Endocrinology & Metabolism 93,1062-1071.
• Raetzman L.T., Ward R., Camper S.A., 2002 − Lhx4 and Prop1 are required for cell survival and expansion of the pituitary primordia. Development 129, 4229-4239.
• Ren G., Chen H., Zhang L.Z., Lan XY, Wei T.B., Li M.J., Jing Y.J., Lei C.Z., Wang , J.Q., 2010 − A coding SNP of LHX4 gene is associated with body weight and body length in bovine.Molecular Biology Reports 37, 417-422.
• Sambrook J., Russell D.W., 2001 − Molecular cloning: A Laboratory Manual. 3nded. N.Y.,Cold Spring Harbor Laboratory Press.
• Sauna Z.E., Kimchi -Sarfaty C., Ambudkar S.V., Gottesman M.M., 2007 − Silent polymorphisms speak: how they affect pharmacogenomics and the treatment of cancer. Cancer Research 67, 9609-9612.
• Sheng H.Z., Moriyama K., Yamashita T., Li H., Potter S.S., Mahon K.A., Westphal H., 1997 − Multistep control of pituitary organogenesis. Science 278, 1809-1812.
• Stachowiak M., Szydlowski M., Cieslak J., Switonski M., 2007 − SNPs in the porcine PPARGC1a gene: interbreed differences and their phenotypic effects. Cellular & Molecular Biology Letters 12, 231-239.
• Tajima T., Hattori T., Nakajima T., Okuhara K., Tsubaki J., Fujieda K., 2007 −A novel missense mutation (P366T) of the LHX4 gene causes severe combined pituitary hormone deficiency with pituitary hypoplasia, ectopic posterior lobe and a poorly developed sella turcica.Endocrine Journal 54, 637-641.
• Vaclavicek A., Hemminki K., Bartram C.R., Wagner K., Wappenschmidt B., Meindl A., Schmutzler R.K., Klaes R., Untch M., Burwinkel B., Forsti A.,2006 − Association of prolactin and its receptor gene regions with familial breast cancer. Journal of Clinical Endocrinology & Metabolism 91, 513-1519.
• Watkins -Chow D.E., Camper S.A., 1998 − How many homeobox genes does it take to make a pituitary gland? Trends in Genetics 14, 284-290.
• Zhang C.L., Wang Y.H., Chen H., Lan X.Y., Lei C.Z., 2007 − Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining. Analytical Biochemistry 365, 286-287.