EN
The aim of this study was to determine the influence of spontaneous fermentation and that with the use of probiotic bacteria and yeast on aflatoxin B1 concentration and the microflora pattern during fermentation. The probiotic preparation used contained bacteria resistant to gastric juice and bile: Lactobacillus paracasei LOCK 0920, Lactobacillus brevis LOCK 0944, Lactobacillus plantarum LOCK 0945, as well as live yeasts Saccharomyces cerevisiae LOCK 0140 of high fermenting capacity. After 6-h fermentation with the probiotic, in feed mixture with a low concentration of aflatoxin B1 (1 mg/kg), the amount of aflatoxin B1 decreased by 55%. In the case of a high concentration (5 mg/kg) the decrease in aflatoxin B1 was about 39%. This tendency was sustained during the following hours of incubation (12th and 24th h). The application of probiotic bacteria and yeasts resulted in the reduction of aerobic spore forming bacteria.