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2010 | 54 | 4 |

Tytuł artykułu

Occurrence of Clostridium perfringens in food chain

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The occurrcnce of Clostridium perfringens in particular links of food chain and their toxic potential was studied. Compound feeding stuffs, poultry, swine, and bovine faeces, food of animal origin, and human faeces were examined microbiologically. The isolated anaerobes were analysed for presence of toxin genes (cpa, cpb, cpb2, etx, iap, and cpe). The presence of C. perfringens on level higher than 1.0 x 10¹ cfu/g were detected in 68% of feed samples, 36% of poultry faeces, 92% of swine faeces, 67% of bovine faeces, 4% of food samples, and in 67% of human faeces. The detected levels of type A C. perfringens strains did not exceed the number of these strains physiologically present in the intestines of birds and mammals. Food and feed samples also revealed good microbiological quality. The identification of toxin type and subtype revealed domination of type A strains and among them the percentage of subtype ß2 strains varied considerably. The second toxotype detected was toxotype E. There were no cpe-positive strains in the analysed food of animal origin.

Wydawca

-

Rocznik

Tom

54

Numer

4

Opis fizyczny

p.571-576,fig.,ref.

Twórcy

autor
  • Department of Hygiene of Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor

Bibliografia

  • 1. Baums C.G., Schotte U., Amtsberg G., Goethe R.: Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Vet Microbiol 2004, 100, 11-16.
  • 2. Borriello S.P., Barclay F.E., Welch A.R., Stringer M.F., Watson G.N.. Williams R.K.., Seal D.V., Sullens K..: Epidemiology of diarrhea caused by enterotoxigenic Clostridium perfringens. J Med Microbiol 1985, 20, 363-372.
  • 3. Collins J.E., Bergeland M.E., Bouley D., Ducommun A.L., Francis D.H., Yeske P.: Diarrhea associated with Clostridium perfringens type A enterotoxin in neonatal pigs. J Vet Diag Invest 1989, 1, 351-353.
  • 4. Crouch E., Golden N.J.: A risk assessment for Clostridium perfringens in ready-to-eat and partially cooked meat and poultry products. www.fsis.usda.gov/PDF/CPerfringens_Risk_Assess_Sep 2005.pdf#page=288.
  • 5. Fisher D.J., Miyamoto K.., Harrison B., Akimoto S., Sarker M.R., McClane B.A.: Association of ß2 toxin production with Clostridium perfringens type A human gastrointestinal disease isolates carrying a plasmid enterotoxin gene. Mol Microbiol 2005, 56, 747-762.
  • 6. Franciosa G., Ferreira J.L., Hatheway C.L.: Detection of type A, B, and E botulism neurotoxin genes in Clostridium botulinum and other Clostridium species by PCR: evidence of unexpected type B toxin genes in type toxigenic organisms. J Clin Microbiol 1994, 32, 1911- 1917.
  • 7. Hart G.B., Lamb R.C., Strauss M.B.: Gas gangrene. J Trauma 1983, 23, 991-1000.
  • 8. Long J.R., Truscott R.B.: Necrotic enteritis in broiler chickens. III. Reproduction of the disease. Can J Comp Med 1976, 40, 53-59.
  • 9. Sawires Y.S., Songer J.G.: Clostridium perfringens: Insight into virulence evolution and population structure. Anaerobe 2006, 12, 23-43.
  • 10. Skjelkvale R., Stringer M.F., Smart J.L.: Enterotoxin production by lecithinase-positive and lecithinase- negative Clostridium perfringens isolated from food poisoning outbreaks and other sources. J Appl Bacteriol 1979, 47, 329-339.
  • 11. Songer J.G., Meer R.M.: Genotyping of Clostridium perfringens by PCR is a useful adjunct to diagnosis of clostridial enteric disease in animals. Anaerobe 1996, 2, 197-203.
  • 12. Songer J.G.: Clostridial enteric diseases of domestic animals. Clin Microbiol Rev 1996, 9, 216-234.
  • 13. Songer J.G.: Clostridia as agents of zoonotic disease. Vet Microbiol 2010, 140, 399-404.
  • 14. Tominaga K., Takeya G., Okada K.: A first case report in Yamaguchi prefecture of the outbreak of the hemorrhagic enteritis necroticans of dairy cattle caused by Clostridium perfringens type A. Yamaguchi J Vet Med 1984, 11, 71- 76.
  • 15. Vu T.L.A.: Incidence of Clostridium botulinum spores in honey and infant food samples collected from Vietnam and Germany. Thesis. Faculty of Agriculture, Goettingen University (Germany), 2006.
  • 16. Wojdat E.: Microbiological quality assessment of animal feedingstuffs with detailed phenotypical and genotypical characterisation of isolated Clostridium perfringens strains, Thesis. National Veterinary Research Institute in Pulawy, Poland, 2006.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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