Germination and vigour of kohlrabi seeds subjected to priming in the presence of Alternaria brassicicola (Schw.) Wiltshire

• Autorzy: Dorna H. , Szopinska D. , Tylkowska K.

Warianty tytułu

PL

Kiełkowanie i wigor nasion kalarepy poddanych kondycjonowaniu w obecności Alternaria brassicicola (Schw.) Wiltshire

• Języki publikacji: EN

Abstrakty

EN

Priming is a treatment applied for seed enhancement. Brassica vegetable seeds are commonly affected by Alternaria brassicicola. The changes in germination and vigour of kohlrabi (Brassica oleracea L. var. gongylodes L.) seeds subjected to two priming methods in the presence of A. brassicicola were investigated. The following treatments were applied: uninoculated non-primed seeds (control), seeds inoculated with A. brassicicola (2∙104 spores ml-1), uninoculated and inoculated seeds hydroprimed for 1–2 days, and uninoculated and inoculated seeds osmoprimed for 1–4 days. The percentage of germinating seeds, germination capacity, the percentages of diseased seedlings and dead seeds were determined. The speed of germination, tetrazolium staining index, and electrical conductivity described seed vigour. The presence of A. brassicicola on/in seeds was confirmed on CW semiselective agar. Osmopriming was conducive to penetration of A. brassicicola into kohlrabi seeds. The presence of the fungus had no influence on the percentage of germinating seeds, but a significantly lower germination capacity and a higher percentage of diseased seedlings and dead seeds were observed after inoculation. Non-primed inoculated seeds germinated faster than uninoculated ones. Despite the presence of A. brassicicola, osmotic priming improved seed vigour as measured by the speed of seed germination and electrical conductivity. The results of the tetrazolium vigour test showed its limited usefulness for seeds heavily infected with A. brassicicola.

PL

Kondycjonowanie nasion jest zabiegiem poprawiającym ich jakość. Nasiona warzyw kapustnych są powszechnie zasiedlone przez grzyb Alternaria brassicicola. W pracy analizowano wpływ dwóch metod kondycjonowania na kiełkowanie i wigor nasion kalarepy (Brassica oleracea L. var. gongylodes L.) inokulowanych A. brassicicola. Badano nasiona nieinokulowane i niekondycjonowane (kontrola), nasiona inokulowane A. brassicicola (2∙104 zarodników·ml-1), nasiona nieinokulowane i inokulowane hydrokondycjonowane przez 1 i 2 dni oraz nasiona nieinokulowane i inokulowane osmokondycjonowane przez 1–4 dni. Określano liczbę nasion kiełkujących, zdolność kiełkowania oraz procent siewek chorych i nasion martwych. Wigor nasion oceniano na podstawie szybkości ich kiełkowania, testu tetrazolinowego oraz testu konduktometrycznego. Zasiedlenie nasion przez A. brassicicola badano na półselektywnej agarowej pożywce CW. Osmokondycjonowanie sprzyjało penetracji nasion przez patogen. Obecność grzyba w/na nasionach nie miała wpływu na procent kiełkujących nasion, ale znacząco zmniejszała ich zdolność kiełkowania, zwiększając jednocześnie liczbę siewek chorych i nasion martwych. Pomimo obecności patogena kondycjonowanie osmotyczne poprawiało szybkość kiełkowania nasion i wyniki testu konduktometrycznego. Wyniki testu tetrazolinowego wykazały jego ograniczoną przydatność do badania wigoru nasion porażonych w dużym stopniu przez A. brassicicola.

Słowa kluczowe

EN

seed germination; germination; seed vigor; kohlrabi; Brassica oleracea var.gongylodes; seed health; Alternaria brassicicola; seed quality; priming; vegetable

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2010
• Tom: 09
• Numer: 1
• Opis fizyczny: p.45-54,fig.,ref.

Twórcy

autor

Dorna H.

• Department of Seed Science and Technology, University of Life Sciences in Poznan, Baranowo, Szamotulska 28, 62-081 Przezmierowo, Poland

autor

Szopinska D.

autor

Tylkowska K.

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